LOCUS HEXB-pGEn1-DEST 6121 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4749..5290 /note="WPRE" misc_recomb 4711..4733 /label=attB2 /note="attB2 site" misc_feature complement(5297..5511) /note="BGH pA" misc_feature complement(5599..5728) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4710 /dnas_title="HEXB-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1725 IMAGE:2967035" /tissue_type="Skin, melanotic melanoma." /clone_lib="NIH_MGC_20" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3166..4710 /dnas_title="HEXB" /gene="HEXB" /gene_synonym="ENC-1AS" /db_xref="GeneID:3074" /db_xref="HGNC:4879" /db_xref="MIM:606873" CDS 3166..4710 /dnas_title="hexosaminidase B (beta polypeptide)" /gene="HEXB" /gene_synonym="ENC-1AS" /codon_start=1 /product="hexosaminidase B (beta polypeptide)" /protein_id="AAH17378.1" /db_xref="GI:16924217" /db_xref="GeneID:3074" /db_xref="HGNC:4879" /db_xref="MIM:606873" /translation="MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAAR APSVSAKPGPALWPLPLSVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRRYH GYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVL KANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTSRHYLPVKI ILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIE YARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSFLT TFFKEISEVFPDQFIHLGGDEVEFKCWESNPKIQDFMRQKGFGTDFKKLESFYIQKVL DIIATINKGSIVWQEVFDDKAKLAPGTIVEVWKDSAYPEELSRVTASGFPVILSAPWY LDLISYGQDWRKYYKVEPLDFGGTQKQKQLFIGGEACLWGEYVDATNLTPRLWPRASA VGERLWSSKDVRDMDDAYDRLTRHRCRMVERGIAAQPLYAGYCNHENM" misc_difference 3224..3224 /dnas_title="HEXB" /gene="HEXB" /gene_synonym="ENC-1AS" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'S' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_feature 3166..4710 /note="HEXB coding region" vector join(4719..6121,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4718 /source="HEXB-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcgctcg ggccccgagc 3181 gtctcggcca agccggggcc ggcgctgtgg cccctgccgc tctcggtgaa gatgaccccg 3241 aacctgctgc atctcgcccc ggagaacttc tacatcagcc acagccccaa ttccacggcg 3301 ggcccctcct gcaccctgct ggaggaagcg tttcgacgat atcatggcta tatttttggt 3361 ttctacaagt ggcatcatga acctgctgaa ttccaggcta aaacccaggt tcagcaactt 3421 cttgtctcaa tcacccttca gtcagagtgt gatgctttcc ccaacatatc ttcagatgag 3481 tcttatactt tacttgtgaa agaaccagtg gctgtcctta aggccaacag agtttgggga 3541 gcattacgag gtttagagac ctttagccag ttagtttatc aagattctta tggaactttc 3601 accatcaatg aatccaccat tattgattct ccaaggtttt ctcacagagg aattttgatt 3661 gatacatcca gacattatct gccagttaag attattctta aaactctgga tgccatggct 3721 tttaataagt ttaatgttct tcactggcac atagttgatg accagtcttt cccatatcag 3781 agcatcactt ttcctgagtt aagcaataaa ggaagctatt ctttgtctca tgtttataca 3841 ccaaatgatg tccgtatggt gattgaatat gccagattac gaggaattcg agtcctgcca 3901 gaatttgata cccctgggca tacactatct tggggaaaag gtcagaaaga cctcctgact 3961 ccatgttaca gtagacaaaa caagttggac tcttttggac ctataaaccc tactctgaat 4021 acaacataca gcttccttac tacatttttc aaagaaatta gtgaggtgtt tccagatcaa 4081 ttcattcatt tgggaggaga tgaagtggaa tttaaatgtt gggaatcaaa tccaaaaatt 4141 caagatttca tgaggcaaaa aggctttggc acagatttta agaaactaga atctttctac 4201 attcaaaagg ttttggatat tattgcaacc ataaacaagg gatccattgt ctggcaggag 4261 gtttttgatg ataaagcaaa gcttgcgccg ggcacaatag ttgaagtatg gaaagacagc 4321 gcatatcctg aggaactcag tagagtcaca gcatctggct tccctgtaat cctttctgct 4381 ccttggtact tagatttgat tagctatgga caagattgga ggaaatacta taaagtggaa 4441 cctcttgatt ttggcggtac tcagaaacag aaacaacttt tcattggtgg agaagcttgt 4501 ctatggggag aatatgtgga tgcaactaac ctcactccaa gattatggcc tcgggcaagt 4561 gctgttggtg agagactctg gagttccaaa gatgtcagag atatggatga cgcctatgac 4621 agactgacaa ggcaccgctg caggatggtc gaacgtggaa tagctgcaca acctctttat 4681 gctggatatt gtaaccatga gaacatgtaa CCAGCTTTCT TGTACAAAGT GGTTGATAGC 4741 TTGGATCCAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT 4801 ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG 4861 CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG 4921 AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA 4981 CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC 5041 CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG 5101 CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT 5161 GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT 5221 CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC 5281 CGCGTCTTCG AGATCTGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC 5341 CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA 5401 TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG 5461 GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ACTCGAGTTA 5521 AGGGCGAATT GCCGATTAGG ATCTTCCTAG AGCATGGCTA CGTAGATAAG TAGCATGGCG 5581 GGTTAATCAT TAACTACAAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG 5641 CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG 5701 CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC TTAATTAAGA GCTCATGGCG CGCCTAGGCC 5761 TTGACGGCCT TCCGCCAATT CGCCCTATAG TGAGTCGTAT TACGTCGCGC TCACTGGCCG 5821 TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG 5881 CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAA ACGCCCTTCC 5941 CAACAGTTGC GCAGCCTGAA TGGCGAATGG GAGCGCCCTG TAGCGGCCAC TCAACCCTAT 6001 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 6061 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA 6121 G //