LOCUS HEXB-pGEn2-DEST 6846 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 5474..6015 /note="WPRE" misc_recomb 5436..5458 /label=attB2 /note="attB2 site" misc_feature complement(6022..6236) /note="BGH pA" misc_feature complement(6324..6453) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" source 3891..5435 /dnas_title="HEXB-pGEn2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1725 IMAGE:2967035" /tissue_type="Skin, melanotic melanoma." /clone_lib="NIH_MGC_20" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3891..5435 /dnas_title="HEXB" /gene="HEXB" /gene_synonym="ENC-1AS" /db_xref="GeneID:3074" /db_xref="HGNC:4879" /db_xref="MIM:606873" CDS 3891..5435 /dnas_title="hexosaminidase B (beta polypeptide)" /gene="HEXB" /gene_synonym="ENC-1AS" /codon_start=1 /product="hexosaminidase B (beta polypeptide)" /protein_id="AAH17378.1" /db_xref="GI:16924217" /db_xref="GeneID:3074" /db_xref="HGNC:4879" /db_xref="MIM:606873" /translation="MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAAR APSVSAKPGPALWPLPLSVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRRYH GYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVL KANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTSRHYLPVKI ILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIE YARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSFLT TFFKEISEVFPDQFIHLGGDEVEFKCWESNPKIQDFMRQKGFGTDFKKLESFYIQKVL DIIATINKGSIVWQEVFDDKAKLAPGTIVEVWKDSAYPEELSRVTASGFPVILSAPWY LDLISYGQDWRKYYKVEPLDFGGTQKQKQLFIGGEACLWGEYVDATNLTPRLWPRASA VGERLWSSKDVRDMDDAYDRLTRHRCRMVERGIAAQPLYAGYCNHENM" misc_difference 3949..3949 /dnas_title="HEXB" /gene="HEXB" /gene_synonym="ENC-1AS" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'S' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_feature 3891..5435 /note="HEXB coding region" vector join(5444..6846,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..5443 /source="HEXB-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc gctcgggccc 3901 cgagcgtctc ggccaagccg gggccggcgc tgtggcccct gccgctctcg gtgaagatga 3961 ccccgaacct gctgcatctc gccccggaga acttctacat cagccacagc cccaattcca 4021 cggcgggccc ctcctgcacc ctgctggagg aagcgtttcg acgatatcat ggctatattt 4081 ttggtttcta caagtggcat catgaacctg ctgaattcca ggctaaaacc caggttcagc 4141 aacttcttgt ctcaatcacc cttcagtcag agtgtgatgc tttccccaac atatcttcag 4201 atgagtctta tactttactt gtgaaagaac cagtggctgt ccttaaggcc aacagagttt 4261 ggggagcatt acgaggttta gagaccttta gccagttagt ttatcaagat tcttatggaa 4321 ctttcaccat caatgaatcc accattattg attctccaag gttttctcac agaggaattt 4381 tgattgatac atccagacat tatctgccag ttaagattat tcttaaaact ctggatgcca 4441 tggcttttaa taagtttaat gttcttcact ggcacatagt tgatgaccag tctttcccat 4501 atcagagcat cacttttcct gagttaagca ataaaggaag ctattctttg tctcatgttt 4561 atacaccaaa tgatgtccgt atggtgattg aatatgccag attacgagga attcgagtcc 4621 tgccagaatt tgatacccct gggcatacac tatcttgggg aaaaggtcag aaagacctcc 4681 tgactccatg ttacagtaga caaaacaagt tggactcttt tggacctata aaccctactc 4741 tgaatacaac atacagcttc cttactacat ttttcaaaga aattagtgag gtgtttccag 4801 atcaattcat tcatttggga ggagatgaag tggaatttaa atgttgggaa tcaaatccaa 4861 aaattcaaga tttcatgagg caaaaaggct ttggcacaga ttttaagaaa ctagaatctt 4921 tctacattca aaaggttttg gatattattg caaccataaa caagggatcc attgtctggc 4981 aggaggtttt tgatgataaa gcaaagcttg cgccgggcac aatagttgaa gtatggaaag 5041 acagcgcata tcctgaggaa ctcagtagag tcacagcatc tggcttccct gtaatccttt 5101 ctgctccttg gtacttagat ttgattagct atggacaaga ttggaggaaa tactataaag 5161 tggaacctct tgattttggc ggtactcaga aacagaaaca acttttcatt ggtggagaag 5221 cttgtctatg gggagaatat gtggatgcaa ctaacctcac tccaagatta tggcctcggg 5281 caagtgctgt tggtgagaga ctctggagtt ccaaagatgt cagagatatg gatgacgcct 5341 atgacagact gacaaggcac cgctgcagga tggtcgaacg tggaatagct gcacaacctc 5401 tttatgctgg atattgtaac catgagaaca tgtaaCCAGC TTTCTTGTAC AAAGTGGTTG 5461 ATAGCTTGGA TCCAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT 5521 TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC 5581 TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT 5641 TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA 5701 CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC 5761 TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC 5821 AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC TGACGTCCTT 5881 TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT 5941 CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC 6001 TCTTCCGCGT CTTCGAGATC TGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT 6061 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA 6121 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG 6181 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGACTCG 6241 AGTTAAGGGC GAATTGCCGA TTAGGATCTT CCTAGAGCAT GGCTACGTAG ATAAGTAGCA 6301 TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT 6361 GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC 6421 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCCTTAAT TAAGAGCTCA TGGCGCGCCT 6481 AGGCCTTGAC GGCCTTCCGC CAATTCGCCC TATAGTGAGT CGTATTACGT CGCGCTCACT 6541 GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT 6601 TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGAAACGCC 6661 CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGAGCG CCCTGTAGCG GCCACTCAAC 6721 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 6781 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA 6841 ATTTAG //