LOCUS HEXDC-pGEc2-DEST 6679 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6157..6286) /note="3'-ITR" misc_feature complement(5855..6069) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5307..5848 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4486..4508 /note="attB2" misc_feature 5286..5291 /note="BstB1 site" misc_feature 4509..5204 /note="Super GFP" misc_feature 5205..5255 /note="AviTag" misc_feature 5256..5279 /note="His Tag" PCR_frame 3002..4486 /note="Reading Frame" /set="Set 1" source 3006..4463 /dnas_title="HEXDC-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:9663 IMAGE:3464710" /tissue_type="Cervix, carcinoma" /clone_lib="NIH_MGC_12" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4463 /dnas_title="HEXDC" /gene="HEXDC" /gene_synonym="FLJ23825" /db_xref="GeneID:284004" /db_xref="HGNC:26307" /db_xref="IMGT/GENE-DB:26307" /db_xref="MIM:26307" misc_difference 3020..3020 /dnas_title="HEXDC" /gene="HEXDC" /gene_synonym="FLJ23825" /note="'G' in cDNA is 'T' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." CDS 3006..4463 /dnas_title="HEXDC protein" /gene="HEXDC" /gene_synonym="FLJ23825" /codon_start=1 /product="HEXDC protein" /protein_id="AAH18205.2" /db_xref="GI:31418138" /db_xref="GeneID:284004" /db_xref="HGNC:26307" /db_xref="IMGT/GENE-DB:26307" /db_xref="MIM:26307" /translation="MEFVLKHTAFAHLREVGSFPCTLNPHEAESLALVGAMIDQVLEL HPGAQRLHIGCDEVYYLGEGEASRRWLQQEQNSTGKLCLSHMRAVASGVKARRPSVTP LVWDDMLRDLPEDQLAASGVPQLVEPVLWDYTADLDVHGKVLLMQKYRRCGFPQLWAA SAFKGATGPSQAVPPVEHHLRNHVQWLQVAGSGPTDSLQGIILTGWQRYDHYSVLCEL LPAGVPSLAACLQLLLRGGFDEDVKAKVENLLGISSLEKTDPVREGAGSFPGSNILAL VTQVSLHLRSSVDALLEGNRYVTGWFSPYHRQRKLIHPVMVQHIQPAALSLLAQWSTL VQELEAALQLAFYPDAVEEWLEENVHPSLQRLQALLQDLSEVSAPPLPPTSPGRDVAQ DP" misc_feature 3006..4463 /note="HEXDC coding region" misc_feature 4464..4484 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4493) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4493) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4494..6679,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4493 /source="HEXDC-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgtc aggttccacg ccatttcaga tgagattagt tcatttagac cttaaaggag 3061 ctccaccaaa ggtctcgtac ctctcagaga tttttcctct gttccgtgcg ctaggtgcaa 3121 acggcctcct cattgagtat gaagacatgt ttccctacga gggccctctg aggctgctga 3181 gggccaagta cgcctacagc ccctctgaaa tcaaagagat cttgcatctg gctggactca 3241 atgagctgga ggtgattccc ttggtgcaga catttggaca catggagttt gtgctgaagc 3301 acacggcctt cgcccacctg cgggaggtgg gctccttccc ctgcaccctg aacccccacg 3361 aggcagagtc cctggcgctg gtgggcgcca tgattgacca ggtcctggag ctacacccag 3421 gcgcccagcg gctgcacatc gggtgtgatg aggtctatta cctcggagag ggggaggcct 3481 cgcgccggtg gctacagcaa gagcagaaca gcacggggaa gttgtgcctg tcacacatgc 3541 gggcggtggc cagcggcgtg aaggcccggc gccccagcgt gacacccctg gtgtgggacg 3601 acatgctccg agacctgcct gaggaccagc tcgcagcgtc cggggtgccg cagctggtgg 3661 agccggtgct ctgggactac acggccgacc tggatgtgca cggcaaggtc ctcctcatgc 3721 agaagtaccg gcggtgcggc tttccgcagc tgtgggcagc cagtgccttc aagggtgcca 3781 cggggcccag ccaggccgtg ccccctgttg agcaccacct caggaaccac gtgcagtggc 3841 tgcaggtggc gggcagcggg cccacggact cactgcaggg catcatcctg accggctggc 3901 agaggtacga ccactactct gtgctgtgcg agctgctgcc cgcaggagtc ccgtccctgg 3961 ccgcctgcct gcagttgctt ctacgcggag gatttgatga agatgttaaa gcgaaagtgg 4021 agaaccttct cgggatttcc agcctggaaa aaacggaccc tgttagggag ggggccggct 4081 ccttccctgg cagcaacatc cttgcccttg tcacacaagt cagcctccat ctgcgcagct 4141 ctgtggatgc gctgctggag ggcaacaggt atgtcactgg ctggttcagc ccctaccacc 4201 gccagcggaa gctcatccac ccggtcatgg ttcagcacat ccagcccgca gcgctcagcc 4261 tcctggcaca gtggagcacc ctcgtgcagg agctggaggc tgccctgcag ctggctttct 4321 acccggatgc cgtggaggag tggctggagg aaaacgtgca ccccagcctg cagcggctgc 4381 aagctctgct gcaggacctc agcgaggtgt ctgccccccc gctgccaccc accagccctg 4441 gcagggacgt tgctcaggac cccgaaaact tgtactttca aggctCCAGC TTTCTTGTAC 4501 AAAGTGGTAT GAGCAAGGGC GAGGAACTGT TCACCGGCGT GGTGCCCATC CTGGTGGAAC 4561 TGGATGGCGA CGTGAACGGC CACAAGTTCT CTGTGCGGGG AGAGGGCGAA GGCGACGCCA 4621 CAAATGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCTTGGC 4681 CTACCCTCGT GACCACACTG ACCTACGGCG TGCAGTGCTT CAGCAGATAC CCCGACCATA 4741 TGAAGCGGCA CGACTTCTTC AAGAGCGCCA TGCCCGAGGG CTACGTGCAG GAACGGACCA 4801 TCAGCTTCAA GGACGACGGC ACCTACAAGA CCAGAGCCGA AGTGAAGTTC GAGGGCGACA 4861 CCCTCGTGAA CCGGATCGAG CTGAAGGGCA TCGATTTCAA AGAGGACGGC AACATCCTGG 4921 GCCACAAGCT GGAGTACAAC TTCAACAGCC ACAACGTGTA CATCACCGCC GACAAGCAGA 4981 AGAACGGCAT CAAGGCCAAC TTCAAGATCC GGCACAACGT GGAAGATGGC AGCGTGCAGC 5041 TGGCCGACCA CTACCAGCAG AACACCCCCA TCGGAGATGG CCCCGTGCTG CTGCCCGACA 5101 ACCACTACCT GAGCACCCAG AGCGTGCTGA GCAAGGACCC CAACGAGAAG CGGGACCACA 5161 TGGTGCTGCT GGAATTCGTG ACCGCCGCTG GCATCACCCA CGGCATGAGC GGCCTGAACG 5221 ACATCTTCGA GGCCCAGAAA ATCGAGTGGC ACGAGCACCA CCATCATCAC CATCACCACT 5281 GATGATTCGA AAAATAATAA GGATCCAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT 5341 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC 5401 CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT 5461 GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA 5521 CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT 5581 CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG 5641 CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA 5701 AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT 5761 CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC 5821 CGGCTCTGCG GCCTCTTCCG CGTCTTCGAG ATCTGCCTCG ACTGTGCCTT CTAGTTGCCA 5881 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC 5941 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT 6001 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA 6061 TGCTGGGGAC TCGAGTTAAG GGCGAATTGC CGATTAGGAT CTTCCTAGAG CATGGCTACG 6121 TAGATAAGTA GCATGGCGGG TTAATCATTA ACTACAAGGA ACCCCTAGTG ATGGAGTTGG 6181 CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC 6241 GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCCTT AATTAAGAGC 6301 TCATGGCGCG CCTAGGCCTT GACGGCCTTC CGCCAATTCG CCCTATAGTG AGTCGTATTA 6361 CGTCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC 6421 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC 6481 GCACCGAAAC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA GCGCCCTGTA 6541 GCGGCCACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC 6601 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT 6661 ATTAACGCTT ACAATTTAG //