LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2153..2249) /label=attL2 /note="attL2 (c)" misc_signal complement(2264..2283) /note="T7 Promoter/priming site (c)" primer_bind 2291..2307 /note="M13 Reverse priming site" gene 2420..3229 /note="Kanamycin resistance gene" rep_origin 3350..4023 /note="pUC origin" PCR_frame 669..2153 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2173) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2170) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2161..4026,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2131..2151 /note="TEV site" source 673..2130 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:9663 IMAGE:3464710" /tissue_type="Cervix, carcinoma" /clone_lib="NIH_MGC_12" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..2130 /gene="HEXDC" /gene_synonym=FLJ23825 /db_xref="GeneID:284004" /db_xref="HGNC:26307" /db_xref="IMGT/GENE-DB:26307" /db_xref="MIM:26307" misc_difference 687..687 /gene="HEXDC" /gene_synonym=FLJ23825 /note="'G' in cDNA is 'T' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." CDS 673..2130 /dnas_title="HEXDC protein" /gene="HEXDC" /gene_synonym=FLJ23825 /codon_start=1 /product="HEXDC protein" /protein_id="AAH18205.2" /db_xref="GI:31418138" /db_xref="GeneID:284004" /db_xref="HGNC:26307" /db_xref="IMGT/GENE-DB:26307" /db_xref="MIM:26307" /translation="MEFVLKHTAFAHLREVGSFPCTLNPHEAESLALVGAMIDQVLEL HPGAQRLHIGCDEVYYLGEGEASRRWLQQEQNSTGKLCLSHMRAVASGVKARRPSVTP LVWDDMLRDLPEDQLAASGVPQLVEPVLWDYTADLDVHGKVLLMQKYRRCGFPQLWAA SAFKGATGPSQAVPPVEHHLRNHVQWLQVAGSGPTDSLQGIILTGWQRYDHYSVLCEL LPAGVPSLAACLQLLLRGGFDEDVKAKVENLLGISSLEKTDPVREGAGSFPGSNILAL VTQVSLHLRSSVDALLEGNRYVTGWFSPYHRQRKLIHPVMVQHIQPAALSLLAQWSTL VQELEAALQLAFYPDAVEEWLEENVHPSLQRLQALLQDLSEVSAPPLPPTSPGRDVAQ DP" misc_feature 673..2130 /note="HEXDC coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgtcagg ttccacgcca tttcagatga gattagttca tttagacctt 721 aaaggagctc caccaaaggt ctcgtacctc tcagagattt ttcctctgtt ccgtgcgcta 781 ggtgcaaacg gcctcctcat tgagtatgaa gacatgtttc cctacgaggg ccctctgagg 841 ctgctgaggg ccaagtacgc ctacagcccc tctgaaatca aagagatctt gcatctggct 901 ggactcaatg agctggaggt gattcccttg gtgcagacat ttggacacat ggagtttgtg 961 ctgaagcaca cggccttcgc ccacctgcgg gaggtgggct ccttcccctg caccctgaac 1021 ccccacgagg cagagtccct ggcgctggtg ggcgccatga ttgaccaggt cctggagcta 1081 cacccaggcg cccagcggct gcacatcggg tgtgatgagg tctattacct cggagagggg 1141 gaggcctcgc gccggtggct acagcaagag cagaacagca cggggaagtt gtgcctgtca 1201 cacatgcggg cggtggccag cggcgtgaag gcccggcgcc ccagcgtgac acccctggtg 1261 tgggacgaca tgctccgaga cctgcctgag gaccagctcg cagcgtccgg ggtgccgcag 1321 ctggtggagc cggtgctctg ggactacacg gccgacctgg atgtgcacgg caaggtcctc 1381 ctcatgcaga agtaccggcg gtgcggcttt ccgcagctgt gggcagccag tgccttcaag 1441 ggtgccacgg ggcccagcca ggccgtgccc cctgttgagc accacctcag gaaccacgtg 1501 cagtggctgc aggtggcggg cagcgggccc acggactcac tgcagggcat catcctgacc 1561 ggctggcaga ggtacgacca ctactctgtg ctgtgcgagc tgctgcccgc aggagtcccg 1621 tccctggccg cctgcctgca gttgcttcta cgcggaggat ttgatgaaga tgttaaagcg 1681 aaagtggaga accttctcgg gatttccagc ctggaaaaaa cggaccctgt tagggagggg 1741 gccggctcct tccctggcag caacatcctt gcccttgtca cacaagtcag cctccatctg 1801 cgcagctctg tggatgcgct gctggagggc aacaggtatg tcactggctg gttcagcccc 1861 taccaccgcc agcggaagct catccacccg gtcatggttc agcacatcca gcccgcagcg 1921 ctcagcctcc tggcacagtg gagcaccctc gtgcaggagc tggaggctgc cctgcagctg 1981 gctttctacc cggatgccgt ggaggagtgg ctggaggaaa acgtgcaccc cagcctgcag 2041 cggctgcaag ctctgctgca ggacctcagc gaggtgtctg cccccccgct gccacccacc 2101 agccctggca gggacgttgc tcaggacccc gaaaacttgt actttcaagg ctCCAGCTTT 2161 CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT 2221 CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT GATATCCCCT ATAGTGAGTC 2281 GTATTACATG GTCATAGCTG TTTCCTGGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG 2341 TTACATTGCA CAAGATAAAA TAATATCATC ATGAACAATA AAACTGTCTG CTTACATAAA 2401 CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA ACGTCGAGGC CGCGATTAAA 2461 TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG TCGGGCAATC 2521 AGGTGCGACA ATCTATCGCT TGTATGGGAA GCCCGATGCG CCAGAGTTGT TTCTGAAACA 2581 TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA ACTGGCTGAC 2641 GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG ATGCATGGTT 2701 ACTCACCACT GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT ATCCTGATTC 2761 AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT CGATTCCTGT 2821 TTGTAATTGT CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC AATCACGAAT 2881 GAATAACGGT TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT GGCCTGTTGA 2941 ACAAGTCTGG AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG TCGTCACTCA 3001 TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG GTTGTATTGA 3061 TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT GGAACTGCCT 3121 CGGTGAGTTT TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA TTGATAATCC 3181 TGATATGAAT AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT CAGAATTGGT 3241 TAATTGGTTG TAACACTGGC AGAGCATTAC GCTGACTTGA CGGGACGGCG CAAGCTCATG 3301 ACCAAAATCC CTTAACGTGA GTTACGCGTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 3361 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 3421 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 3481 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA 3541 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 3601 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 3661 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 3721 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 3781 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 3841 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 3901 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 3961 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 4021 CATGTT //