LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1139..1235)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1250..1269)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1277..1293
                     /note="M13 Reverse priming site"
     gene            1406..2215
                     /note="Kanamycin resistance gene"
     rep_origin      2336..3009
                     /note="pUC origin"
     PCR_frame       669..1139
                     /note="Reading Frame"
                     /set="Set 1"
     PCR_primer      652..668
                     /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC"
                     /set="Set 1"
                     /pair="Pair 1"
                     /current=1
                     /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC
     PCR_primer      complement(669..1159)
                     /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG"
                     /set="Set 1"
                     /pair="Pair 1"
                     /current=1
                     /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG
     PCR_product     complement(652..1156)
                     /pair="Pair 1"
                     /set="Set 1"
                     /current=1
                     /score=1.000
     vector          join(1147..3012,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    1117..1137
                     /note="TEV site"
     source          673..1116
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:2337 IMAGE:2959387"
                     /tissue_type="Colon, adenocarcinoma"
                     /clone_lib="NIH_MGC_15"
                     /lab_host="DH10B-R"
                     /note="Vector: pOTB7"
     gene            673..1116
                     /gene="LYZ"
                     /db_xref="GeneID:4069"
                     /db_xref="HGNC:6740"
                     /db_xref="MIM:153450"
     CDS             673..1116
                     /dnas_title="lysozyme (renal amyloidosis)"
                     /gene="LYZ"
                     /codon_start=1
                     /product="lysozyme (renal amyloidosis)"
                     /protein_id="AAH04147.1"
                     /db_xref="GI:13278744"
                     /db_xref="GeneID:4069"
                     /db_xref="HGNC:6740"
                     /db_xref="MIM:153450"
                     /translation="MKALIVLGLVLLSVTVQGKVFERCELARTLKRLGMDGYRGISLA
                     NWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCSAL
                     LQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV"
     misc_feature    673..1116
                     /note="LYZ coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTCG CCatgaaggc tctcattgtt ctggggcttg tcctcctttc tgttacggtc
        721 cagggcaagg tctttgaaag gtgtgagttg gccagaactc tgaaaagatt gggaatggat
        781 ggctacaggg gaatcagcct agcaaactgg atgtgtttgg ccaaatggga gagtggttac
        841 aacacacgag ctacaaacta caatgctgga gacagaagca ctgattatgg gatatttcag
        901 atcaatagcc gctactggtg taatgatggc aaaaccccag gagcagttaa tgcctgtcat
        961 ttatcctgca gtgctttgct gcaagataac atcgctgatg ctgtagcttg tgcaaagagg
       1021 gttgtccgtg atccacaagg cattagagca tgggtggcat ggagaaatcg ttgtcaaaac
       1081 agagatgtcc gtcagtatgt tcaaggttgt ggagtggaaa acttgtactt tcaaggctCC
       1141 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA
       1201 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG
       1261 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT
       1321 CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA ACAATAAAAC TGTCTGCTTA
       1381 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG
       1441 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG
       1501 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT
       1561 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG
       1621 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC
       1681 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC
       1741 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT
       1801 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC
       1861 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC
       1921 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT
       1981 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG
       2041 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA
       2101 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA
       2161 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA
       2221 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG
       2281 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT
       2341 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
       2401 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
       2461 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA
       2521 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
       2581 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
       2641 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
       2701 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA
       2761 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
       2821 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
       2881 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
       2941 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
       3001 TGCTCACATG TT
//