LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1085..1181)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1196..1215)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1223..1239
                     /note="M13 Reverse priming site"
     gene            1352..2161
                     /note="Kanamycin resistance gene"
     rep_origin      2282..2955
                     /note="pUC origin"
     vector          join(1093..2958,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1084
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:2337 IMAGE:2959387"
                     /tissue_type="Colon, adenocarcinoma"
                     /clone_lib="NIH_MGC_15"
                     /lab_host="DH10B-R"
                     /note="Vector: pOTB7"
     gene            692..1084
                     /gene="LYZ"
                     /db_xref="GeneID:4069"
                     /db_xref="HGNC:6740"
                     /db_xref="MIM:153450"
     CDS             692..1084
                     /dnas_title="lysozyme (renal amyloidosis)"
                     /gene="LYZ"
                     /codon_start=1
                     /product="lysozyme (renal amyloidosis)"
                     /protein_id="AAH04147.1"
                     /db_xref="GI:13278744"
                     /db_xref="GeneID:4069"
                     /db_xref="HGNC:6740"
                     /db_xref="MIM:153450"
                     /translation="MKALIVLGLVLLSVTVQGKVFERCELARTLKRLGMDGYRGISLA
                     NWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCSAL
                     LQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV"
     misc_feature    692..1084
                     /note="LYZ coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg caaggtcttt gaaaggtgtg agttggccag
        721 aactctgaaa agattgggaa tggatggcta caggggaatc agcctagcaa actggatgtg
        781 tttggccaaa tgggagagtg gttacaacac acgagctaca aactacaatg ctggagacag
        841 aagcactgat tatgggatat ttcagatcaa tagccgctac tggtgtaatg atggcaaaac
        901 cccaggagca gttaatgcct gtcatttatc ctgcagtgct ttgctgcaag ataacatcgc
        961 tgatgctgta gcttgtgcaa agagggttgt ccgtgatcca caaggcatta gagcatgggt
       1021 ggcatggaga aatcgttgtc aaaacagaga tgtccgtcag tatgttcaag gttgtggagt
       1081 gtaaCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG
       1141 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC
       1201 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA
       1261 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC
       1321 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG
       1381 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA
       1441 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT
       1501 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT
       1561 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA
       1621 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA
       1681 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA
       1741 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC
       1801 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG
       1861 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC
       1921 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT
       1981 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT
       2041 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG
       2101 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA
       2161 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG
       2221 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA
       2281 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG
       2341 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC
       2401 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT
       2461 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC
       2521 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT
       2581 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG
       2641 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT
       2701 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG
       2761 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG
       2821 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
       2881 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG
       2941 GCCTTTTGCT CACATGTT
//