LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1133..1229) /label=attL2 /note="attL2 (c)" misc_signal complement(1244..1263) /note="T7 Promoter/priming site (c)" primer_bind 1271..1287 /note="M13 Reverse priming site" gene 1400..2209 /note="Kanamycin resistance gene" rep_origin 2330..3003 /note="pUC origin" PCR_frame 669..1133 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..1153) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1150) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1141..3006,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1111..1131 /note="TEV site" source 673..1110 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:26768 IMAGE:4830396" /tissue_type="Testis" /clone_lib="NIH_MGC_97" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 673..1110 /gene="LYZL4" /gene_synonym=1810009N24Rik /gene_synonym=LYC4 /gene_synonym=MGC26768 /db_xref="GeneID:131375" /db_xref="HGNC:28387" CDS 673..1110 /dnas_title="lysozyme-like 4" /gene="LYZL4" /gene_synonym=1810009N24Rik /gene_synonym=LYC4 /gene_synonym=MGC26768 /codon_start=1 /product="lysozyme-like 4" /protein_id="AAH16747.1" /db_xref="GI:16876939" /db_xref="GeneID:131375" /db_xref="HGNC:28387" /translation="MKASVVLSLLGYLVVPSGAYILGRCTVAKKLHDGGLDYFEGYSL ENWVCLAYFESKFNPMAIYENTREGYTGFGLFQMRGSDWCGDHGRNRCHMSCSALLNP NLEKTIKCAKTIVKGKEGMGAWPTWSRYCQYSDTLARWLDGCKL" misc_feature 673..1110 /note="LYZL4" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgaaggc atccgtggtt ctctccctcc ttggctacct ggtggttcca 721 agtggtgctt acatcttggg gcgttgcaca gtggctaaga aactccacga tggaggcctg 781 gattattttg agggctatag ccttgagaac tgggtgtgcc tggcctactt cgagagcaag 841 ttcaacccca tggccatcta cgagaacaca cgtgagggct acactggctt tggcctcttt 901 cagatgcgtg gcagtgactg gtgtggcgac catggcagga accgctgcca tatgtcatgt 961 tccgctttac tgaatcctaa tttagagaag acaattaaat gtgccaagac cattgtaaaa 1021 ggaaaagaag ggatgggagc atggcccacc tggtcccggt actgccagta ctccgatacc 1081 ctggcacggt ggctggatgg ttgcaagctg gaaaacttgt actttcaagg ctCCAGCTTT 1141 CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT 1201 CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT GATATCCCCT ATAGTGAGTC 1261 GTATTACATG GTCATAGCTG TTTCCTGGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG 1321 TTACATTGCA CAAGATAAAA TAATATCATC ATGAACAATA AAACTGTCTG CTTACATAAA 1381 CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA ACGTCGAGGC CGCGATTAAA 1441 TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG TCGGGCAATC 1501 AGGTGCGACA ATCTATCGCT TGTATGGGAA GCCCGATGCG CCAGAGTTGT TTCTGAAACA 1561 TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA ACTGGCTGAC 1621 GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG ATGCATGGTT 1681 ACTCACCACT GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT ATCCTGATTC 1741 AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT CGATTCCTGT 1801 TTGTAATTGT CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC AATCACGAAT 1861 GAATAACGGT TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT GGCCTGTTGA 1921 ACAAGTCTGG AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG TCGTCACTCA 1981 TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG GTTGTATTGA 2041 TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT GGAACTGCCT 2101 CGGTGAGTTT TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA TTGATAATCC 2161 TGATATGAAT AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT CAGAATTGGT 2221 TAATTGGTTG TAACACTGGC AGAGCATTAC GCTGACTTGA CGGGACGGCG CAAGCTCATG 2281 ACCAAAATCC CTTAACGTGA GTTACGCGTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 2341 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 2401 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 2461 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA 2521 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 2581 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 2641 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 2701 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 2761 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 2821 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 2881 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 2941 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 3001 CATGTT //