LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1982..2078) /label=attL2 /note="attL2 (c)" misc_signal complement(2093..2112) /note="T7 Promoter/priming site (c)" primer_bind 2120..2136 /note="M13 Reverse priming site" gene 2249..3058 /note="Kanamycin resistance gene" rep_origin 3179..3852 /note="pUC origin" PCR_frame 669..1982 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2002) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1999) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1990..3855,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1960..1980 /note="TEV site" source 673..1959 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:4162 IMAGE:3609235" /tissue_type="Uterus, endometrium adenocarcinoma" /clone_lib="NIH_MGC_44" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..1959 /gene="GLA" /gene_synonym=GALA /db_xref="GeneID:2717" /db_xref="HGNC:4296" /db_xref="MIM:301500" CDS 673..1959 /dnas_title="galactosidase, alpha" /gene="GLA" /gene_synonym=GALA /codon_start=1 /product="galactosidase, alpha" /protein_id="AAH02689.1" /db_xref="GI:12803707" /db_xref="GeneID:2717" /db_xref="HGNC:4296" /db_xref="MIM:301500" /translation="MQLRNPELHLGCALALRFLALVSWDIPGARALDNGLARTPTMGW LHWERFMCNLDCQEEPDSCISEKLFMEMAELMVSEGWKDAGYEYLCIDDCWMAPQRDS EGRLQADPQRFPHGIRQLANYVHSKGLKLGIYADVGNKTCAGFPGSFGYYDIDAQTFA DWGVDLLKFDGCYCDSLENLADGYKHMSLALNRTGRSIVYSCEWPLYMWPFQKPNYTE IRQYCNHWRNFADIDDSWKSIKSILDWTSFNQERIVDVAGPGGWNDPDMLVIGNFGLS WNQQVTQMALWAIMAAPLFMSNDLRHISPQAKALLQDKDVIAINQDPLGKQGYQLRQG DNFEVWERPLSGLAWAVAMINRQEIGGPRSYTIAVASLGKGVACNPACFITQLLPVKR KLGFYEWTSRLRSHINPTGTVLLQLENTMQMSLKDLL" misc_feature 673..1959 /note="GLA coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgcagct gaggaaccca gaactacatc tgggctgcgc gcttgcgctt 721 cgcttcctgg ccctcgtttc ctgggacatc cctggggcta gagcactgga caatggattg 781 gcaaggacgc ctaccatggg ctggctgcac tgggagcgct tcatgtgcaa ccttgactgc 841 caggaagagc cagattcctg catcagtgag aagctcttca tggagatggc agagctcatg 901 gtctcagaag gctggaagga tgcaggttat gagtacctct gcattgatga ctgttggatg 961 gctccccaaa gagattcaga aggcagactt caggcagacc ctcagcgctt tcctcatggg 1021 attcgccagc tagctaatta tgttcacagc aaaggactga agctagggat ttatgcagat 1081 gttggaaata aaacctgcgc aggcttccct gggagttttg gatactacga cattgatgcc 1141 cagacctttg ctgactgggg agtagatctg ctaaaatttg atggttgtta ctgtgacagt 1201 ttggaaaatt tggcagatgg ttataagcac atgtccttgg ccctgaatag gactggcaga 1261 agcattgtgt actcctgtga gtggcctctt tatatgtggc cctttcaaaa gcccaattat 1321 acagaaatcc gacagtactg caatcactgg cgaaattttg ctgacattga tgattcctgg 1381 aaaagtataa agagtatctt ggactggaca tcttttaacc aggagagaat tgttgatgtt 1441 gctggaccag ggggttggaa tgacccagat atgttagtga ttggcaactt tggcctcagc 1501 tggaatcagc aagtaactca gatggccctc tgggctatca tggctgctcc tttattcatg 1561 tctaatgacc tccgacacat cagccctcaa gccaaagctc tccttcagga taaggacgta 1621 attgccatca atcaggaccc cttgggcaag caagggtacc agcttagaca gggagacaac 1681 tttgaagtgt gggaacgacc tctctcaggc ttagcctggg ctgtagctat gataaaccgg 1741 caggagattg gtggacctcg ctcttatacc atcgcagttg cttccctggg taaaggagtg 1801 gcctgtaatc ctgcctgctt catcacacag ctcctccctg tgaaaaggaa gctagggttc 1861 tatgaatgga cttcaaggtt aagaagtcac ataaatccca caggcactgt tttgcttcag 1921 ctagaaaata caatgcagat gtcattaaaa gacttacttg aaaacttgta ctttcaaggc 1981 tCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 2041 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2101 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2161 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2221 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2281 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2341 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2401 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2461 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2521 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2581 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2641 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 2701 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 2761 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 2821 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 2881 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 2941 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 3001 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3061 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3121 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3181 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3241 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3301 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3361 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3421 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3481 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3541 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3601 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3661 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3721 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3781 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 3841 TTTTGCTCAC ATGTT //