LOCUS GLA-pGEc2-DEST 6508 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5986..6115) /note="3'-ITR" misc_feature complement(5684..5898) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5136..5677 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4315..4337 /note="attB2" misc_feature 5115..5120 /note="BstB1 site" misc_feature 4338..5033 /note="Super GFP" misc_feature 5034..5084 /note="AviTag" misc_feature 5085..5108 /note="His Tag" PCR_frame 3002..4315 /note="Reading Frame" /set="Set 1" source 3006..4292 /dnas_title="GLA-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:4162 IMAGE:3609235" /tissue_type="Uterus, endometrium adenocarcinoma" /clone_lib="NIH_MGC_44" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4292 /dnas_title="GLA" /gene="GLA" /gene_synonym="GALA" /db_xref="GeneID:2717" /db_xref="HGNC:4296" /db_xref="MIM:301500" CDS 3006..4292 /dnas_title="galactosidase, alpha" /gene="GLA" /gene_synonym="GALA" /codon_start=1 /product="galactosidase, alpha" /protein_id="AAH02689.1" /db_xref="GI:12803707" /db_xref="GeneID:2717" /db_xref="HGNC:4296" /db_xref="MIM:301500" /translation="MQLRNPELHLGCALALRFLALVSWDIPGARALDNGLARTPTMGW LHWERFMCNLDCQEEPDSCISEKLFMEMAELMVSEGWKDAGYEYLCIDDCWMAPQRDS EGRLQADPQRFPHGIRQLANYVHSKGLKLGIYADVGNKTCAGFPGSFGYYDIDAQTFA DWGVDLLKFDGCYCDSLENLADGYKHMSLALNRTGRSIVYSCEWPLYMWPFQKPNYTE IRQYCNHWRNFADIDDSWKSIKSILDWTSFNQERIVDVAGPGGWNDPDMLVIGNFGLS WNQQVTQMALWAIMAAPLFMSNDLRHISPQAKALLQDKDVIAINQDPLGKQGYQLRQG DNFEVWERPLSGLAWAVAMINRQEIGGPRSYTIAVASLGKGVACNPACFITQLLPVKR KLGFYEWTSRLRSHINPTGTVLLQLENTMQMSLKDLL" misc_feature 3006..4292 /note="GLA coding region" misc_feature 4293..4313 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4322) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4322) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4323..6508,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4322 /source="GLA-Cterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgca gctgaggaac ccagaactac atctgggctg cgcgcttgcg cttcgcttcc 3061 tggccctcgt ttcctgggac atccctgggg ctagagcact ggacaatgga ttggcaagga 3121 cgcctaccat gggctggctg cactgggagc gcttcatgtg caaccttgac tgccaggaag 3181 agccagattc ctgcatcagt gagaagctct tcatggagat ggcagagctc atggtctcag 3241 aaggctggaa ggatgcaggt tatgagtacc tctgcattga tgactgttgg atggctcccc 3301 aaagagattc agaaggcaga cttcaggcag accctcagcg ctttcctcat gggattcgcc 3361 agctagctaa ttatgttcac agcaaaggac tgaagctagg gatttatgca gatgttggaa 3421 ataaaacctg cgcaggcttc cctgggagtt ttggatacta cgacattgat gcccagacct 3481 ttgctgactg gggagtagat ctgctaaaat ttgatggttg ttactgtgac agtttggaaa 3541 atttggcaga tggttataag cacatgtcct tggccctgaa taggactggc agaagcattg 3601 tgtactcctg tgagtggcct ctttatatgt ggccctttca aaagcccaat tatacagaaa 3661 tccgacagta ctgcaatcac tggcgaaatt ttgctgacat tgatgattcc tggaaaagta 3721 taaagagtat cttggactgg acatctttta accaggagag aattgttgat gttgctggac 3781 cagggggttg gaatgaccca gatatgttag tgattggcaa ctttggcctc agctggaatc 3841 agcaagtaac tcagatggcc ctctgggcta tcatggctgc tcctttattc atgtctaatg 3901 acctccgaca catcagccct caagccaaag ctctccttca ggataaggac gtaattgcca 3961 tcaatcagga ccccttgggc aagcaagggt accagcttag acagggagac aactttgaag 4021 tgtgggaacg acctctctca ggcttagcct gggctgtagc tatgataaac cggcaggaga 4081 ttggtggacc tcgctcttat accatcgcag ttgcttccct gggtaaagga gtggcctgta 4141 atcctgcctg cttcatcaca cagctcctcc ctgtgaaaag gaagctaggg ttctatgaat 4201 ggacttcaag gttaagaagt cacataaatc ccacaggcac tgttttgctt cagctagaaa 4261 atacaatgca gatgtcatta aaagacttac ttgaaaactt gtactttcaa ggctCCAGCT 4321 TTCTTGTACA AAGTGGTATG AGCAAGGGCG AGGAACTGTT CACCGGCGTG GTGCCCATCC 4381 TGGTGGAACT GGATGGCGAC GTGAACGGCC ACAAGTTCTC TGTGCGGGGA GAGGGCGAAG 4441 GCGACGCCAC AAATGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG 4501 TGCCTTGGCC TACCCTCGTG ACCACACTGA CCTACGGCGT GCAGTGCTTC AGCAGATACC 4561 CCGACCATAT GAAGCGGCAC GACTTCTTCA AGAGCGCCAT GCCCGAGGGC TACGTGCAGG 4621 AACGGACCAT CAGCTTCAAG GACGACGGCA CCTACAAGAC CAGAGCCGAA GTGAAGTTCG 4681 AGGGCGACAC CCTCGTGAAC CGGATCGAGC TGAAGGGCAT CGATTTCAAA GAGGACGGCA 4741 ACATCCTGGG CCACAAGCTG GAGTACAACT TCAACAGCCA CAACGTGTAC ATCACCGCCG 4801 ACAAGCAGAA GAACGGCATC AAGGCCAACT TCAAGATCCG GCACAACGTG GAAGATGGCA 4861 GCGTGCAGCT GGCCGACCAC TACCAGCAGA ACACCCCCAT CGGAGATGGC CCCGTGCTGC 4921 TGCCCGACAA CCACTACCTG AGCACCCAGA GCGTGCTGAG CAAGGACCCC AACGAGAAGC 4981 GGGACCACAT GGTGCTGCTG GAATTCGTGA CCGCCGCTGG CATCACCCAC GGCATGAGCG 5041 GCCTGAACGA CATCTTCGAG GCCCAGAAAA TCGAGTGGCA CGAGCACCAC CATCATCACC 5101 ATCACCACTG ATGATTCGAA AAATAATAAG GATCCAATCA ACCTCTGGAT TACAAAATTT 5161 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 5221 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 5281 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG 5341 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC 5401 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG 5461 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT 5521 TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC 5581 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG 5641 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGAGA TCTGCCTCGA CTGTGCCTTC 5701 TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC 5761 CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG 5821 TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA 5881 TAGCAGGCAT GCTGGGGACT CGAGTTAAGG GCGAATTGCC GATTAGGATC TTCCTAGAGC 5941 ATGGCTACGT AGATAAGTAG CATGGCGGGT TAATCATTAA CTACAAGGAA CCCCTAGTGA 6001 TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG 6061 TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCCTTA 6121 ATTAAGAGCT CATGGCGCGC CTAGGCCTTG ACGGCCTTCC GCCAATTCGC CCTATAGTGA 6181 GTCGTATTAC GTCGCGCTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG 6241 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG 6301 AAGAGGCCCG CACCGAAACG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAG 6361 CGCCCTGTAG CGGCCACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 6421 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT 6481 TAACAAAATA TTAACGCTTA CAATTTAG //