LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1889..1985)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(2000..2019)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     2027..2043
                     /note="M13 Reverse priming site"
     gene            2156..2965
                     /note="Kanamycin resistance gene"
     rep_origin      3086..3759
                     /note="pUC origin"
     vector          join(1897..3762,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1888
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:4162 IMAGE:3609235"
                     /tissue_type="Uterus, endometrium adenocarcinoma"
                     /clone_lib="NIH_MGC_44"
                     /lab_host="DH10B-R"
                     /note="Vector: pOTB7"
     gene            692..1888
                     /gene="GLA"
                     /gene_synonym=GALA
                     /db_xref="GeneID:2717"
                     /db_xref="HGNC:4296"
                     /db_xref="MIM:301500"
     CDS             692..1888
                     /dnas_title="galactosidase, alpha"
                     /gene="GLA"
                     /gene_synonym=GALA
                     /codon_start=1
                     /product="galactosidase, alpha"
                     /protein_id="AAH02689.1"
                     /db_xref="GI:12803707"
                     /db_xref="GeneID:2717"
                     /db_xref="HGNC:4296"
                     /db_xref="MIM:301500"
                     /translation="MQLRNPELHLGCALALRFLALVSWDIPGARALDNGLARTPTMGW
                     LHWERFMCNLDCQEEPDSCISEKLFMEMAELMVSEGWKDAGYEYLCIDDCWMAPQRDS
                     EGRLQADPQRFPHGIRQLANYVHSKGLKLGIYADVGNKTCAGFPGSFGYYDIDAQTFA
                     DWGVDLLKFDGCYCDSLENLADGYKHMSLALNRTGRSIVYSCEWPLYMWPFQKPNYTE
                     IRQYCNHWRNFADIDDSWKSIKSILDWTSFNQERIVDVAGPGGWNDPDMLVIGNFGLS
                     WNQQVTQMALWAIMAAPLFMSNDLRHISPQAKALLQDKDVIAINQDPLGKQGYQLRQG
                     DNFEVWERPLSGLAWAVAMINRQEIGGPRSYTIAVASLGKGVACNPACFITQLLPVKR
                     KLGFYEWTSRLRSHINPTGTVLLQLENTMQMSLKDLL"
     misc_feature    692..1888
                     /note="GLA coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cctggacaat ggattggcaa ggacgcctac
        721 catgggctgg ctgcactggg agcgcttcat gtgcaacctt gactgccagg aagagccaga
        781 ttcctgcatc agtgagaagc tcttcatgga gatggcagag ctcatggtct cagaaggctg
        841 gaaggatgca ggttatgagt acctctgcat tgatgactgt tggatggctc cccaaagaga
        901 ttcagaaggc agacttcagg cagaccctca gcgctttcct catgggattc gccagctagc
        961 taattatgtt cacagcaaag gactgaagct agggatttat gcagatgttg gaaataaaac
       1021 ctgcgcaggc ttccctggga gttttggata ctacgacatt gatgcccaga cctttgctga
       1081 ctggggagta gatctgctaa aatttgatgg ttgttactgt gacagtttgg aaaatttggc
       1141 agatggttat aagcacatgt ccttggccct gaataggact ggcagaagca ttgtgtactc
       1201 ctgtgagtgg cctctttata tgtggccctt tcaaaagccc aattatacag aaatccgaca
       1261 gtactgcaat cactggcgaa attttgctga cattgatgat tcctggaaaa gtataaagag
       1321 tatcttggac tggacatctt ttaaccagga gagaattgtt gatgttgctg gaccaggggg
       1381 ttggaatgac ccagatatgt tagtgattgg caactttggc ctcagctgga atcagcaagt
       1441 aactcagatg gccctctggg ctatcatggc tgctccttta ttcatgtcta atgacctccg
       1501 acacatcagc cctcaagcca aagctctcct tcaggataag gacgtaattg ccatcaatca
       1561 ggaccccttg ggcaagcaag ggtaccagct tagacaggga gacaactttg aagtgtggga
       1621 acgacctctc tcaggcttag cctgggctgt agctatgata aaccggcagg agattggtgg
       1681 acctcgctct tataccatcg cagttgcttc cctgggtaaa ggagtggcct gtaatcctgc
       1741 ctgcttcatc acacagctcc tccctgtgaa aaggaagcta gggttctatg aatggacttc
       1801 aaggttaaga agtcacataa atcccacagg cactgttttg cttcagctag aaaatacaat
       1861 gcagatgtca ttaaaagact tactttaaCC AGCTTTCTTG TACAAAGTTG GCATTATAAG
       1921 AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT
       1981 TATTTGCCAT CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC
       2041 CTGGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAATAAT
       2101 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG
       2161 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA
       2221 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA
       2281 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA
       2341 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT
       2401 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA
       2461 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT
       2521 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA
       2581 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG
       2641 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA
       2701 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT
       2761 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA
       2821 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA
       2881 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA
       2941 TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG
       3001 CATTACGCTG ACTTGACGGG ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA
       3061 CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
       3121 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
       3181 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
       3241 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
       3301 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
       3361 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
       3421 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
       3481 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
       3541 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
       3601 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
       3661 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
       3721 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT
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