LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2093..2189) /label=attL2 /note="attL2 (c)" misc_signal complement(2204..2223) /note="T7 Promoter/priming site (c)" primer_bind 2231..2247 /note="M13 Reverse priming site" gene 2360..3169 /note="Kanamycin resistance gene" rep_origin 3290..3963 /note="pUC origin" PCR_frame 669..2093 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2113) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2110) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2101..3966,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2071..2091 /note="TEV site" source 673..2070 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:29579 IMAGE:4871788" /tissue_type="Skin, melanotic melanoma, high MDR." /clone_lib="NIH_MGC_49" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2070 /gene="FUCA1" /db_xref="GeneID:2517" /db_xref="HGNC:4006" /db_xref="MIM:230000" CDS 688..2070 /dnas_title="fucosidase, alpha-L- 1, tissue" /gene="FUCA1" /codon_start=1 /product="fucosidase, alpha-L- 1, tissue" /protein_id="AAH17338.1" /db_xref="GI:16878284" /db_xref="GeneID:2517" /db_xref="HGNC:4006" /db_xref="MIM:230000" /translation="MRSRPAGPALLLLLLFLGAAESVRRAQPPRRYTPDWPSLDSRPL PAWFDEAKFGVFIHWGVFSVPAWGSEWFWWHWQGEGRPQYQRFMRDNYPPGFSYADFG PQFTARFFHPEEWADLFQAAGAKYVVLTTKHHEGFTNWPSPVSWNWNSKDVGPHRDLV GELGTALRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVSAKTMPELYDLVNSYK PDLIWSDGEWECPDTYWNSTNFLSWLYNDSPVKDEVVVNDRWGQNCSCHHGGYYNCED KFKPQSLPDHKWEMCTSIDKFSWGYRRDMALSDVTEESEIISELVQTVSLGGNYLLNI GPTKDGLIVPIFQERLLAVGKWLSINGEAIYASKPWRVQWEKNTTSVWYTSKGSAVYA IFLHWPENGVLNLESPITTSTTKITMLGIQGDLKWSTDPDKGLFISLPQLPPSAVPAE FAWTIKLTGVK" misc_feature 673..2070 /note="FUCA1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgcgggc tccggggatg aggtcgcggc cggcgggtcc cgcgctgttg 721 ctgctgctgc tcttcctcgg agcggccgag tcggtgcgtc gggcccagcc tccgcgccgc 781 tacaccccag actggccgag cctggattct cggccgctgc cggcctggtt cgacgaagcc 841 aagttcgggg tgttcatcca ctggggcgtg ttctcggtgc ccgcctgggg cagcgagtgg 901 ttctggtggc actggcaggg cgaggggcgg ccgcagtacc agcgcttcat gcgcgacaac 961 tacccgcccg gcttcagcta cgccgacttc ggaccgcagt tcactgcgcg cttcttccac 1021 ccggaggagt gggccgacct cttccaggcc gcgggcgcca agtatgtagt tttgacgaca 1081 aagcatcacg aaggcttcac aaactggccg agtcctgtgt cttggaactg gaactccaaa 1141 gacgtggggc ctcatcggga tttggttggt gaattgggaa cagctctccg gaagaggaac 1201 atccgctatg gactatacca ctcactctta gagtggttcc atccactcta tctacttgat 1261 aagaaaaatg gcttcaaaac acagcatttt gtcagtgcaa aaacaatgcc agagctgtac 1321 gaccttgtta acagctataa acctgatctg atctggtctg atggggagtg ggaatgtcct 1381 gatacttact ggaactccac aaattttctt tcatggctct acaatgacag ccctgtcaag 1441 gatgaggtgg tagtaaatga ccgatggggt cagaactgtt cctgtcacca tggaggatac 1501 tataactgtg aagataaatt caagccacag agcttgccag atcacaagtg ggagatgtgc 1561 accagcattg acaagttttc ctggggctat cgtcgtgaca tggcattgtc tgatgttaca 1621 gaagaatctg aaatcatttc ggaactggtt cagacagtaa gtttgggagg caactatctt 1681 ctgaacattg gaccaactaa agatggactg attgttccca tcttccaaga aaggcttctt 1741 gctgttggga aatggctgag catcaatggg gaggctatct atgcctccaa accatggcgg 1801 gtgcaatggg aaaagaacac aacatctgta tggtatacct caaagggatc ggctgtttat 1861 gccatttttc tgcactggcc agaaaatgga gtcttaaacc ttgaatcccc cataactacc 1921 tcaactacaa agataacaat gctgggaatt caaggagatc tgaagtggtc cacagatcca 1981 gataaaggtc tcttcatctc tctaccccag ttgccaccct ctgctgtccc cgcagagttt 2041 gcttggacta taaagctgac aggagtgaag gaaaacttgt actttcaagg ctCCAGCTTT 2101 CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT 2161 CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT GATATCCCCT ATAGTGAGTC 2221 GTATTACATG GTCATAGCTG TTTCCTGGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG 2281 TTACATTGCA CAAGATAAAA TAATATCATC ATGAACAATA AAACTGTCTG CTTACATAAA 2341 CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA ACGTCGAGGC CGCGATTAAA 2401 TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG TCGGGCAATC 2461 AGGTGCGACA ATCTATCGCT TGTATGGGAA GCCCGATGCG CCAGAGTTGT TTCTGAAACA 2521 TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA ACTGGCTGAC 2581 GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG ATGCATGGTT 2641 ACTCACCACT GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT ATCCTGATTC 2701 AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT CGATTCCTGT 2761 TTGTAATTGT CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC AATCACGAAT 2821 GAATAACGGT TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT GGCCTGTTGA 2881 ACAAGTCTGG AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG TCGTCACTCA 2941 TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG GTTGTATTGA 3001 TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT GGAACTGCCT 3061 CGGTGAGTTT TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA TTGATAATCC 3121 TGATATGAAT AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT CAGAATTGGT 3181 TAATTGGTTG TAACACTGGC AGAGCATTAC GCTGACTTGA CGGGACGGCG CAAGCTCATG 3241 ACCAAAATCC CTTAACGTGA GTTACGCGTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 3301 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 3361 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 3421 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA 3481 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 3541 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 3601 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 3661 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 3721 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 3781 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 3841 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 3901 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 3961 CATGTT 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