LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2000..2096) /label=attL2 /note="attL2 (c)" misc_signal complement(2111..2130) /note="T7 Promoter/priming site (c)" primer_bind 2138..2154 /note="M13 Reverse priming site" gene 2267..3076 /note="Kanamycin resistance gene" rep_origin 3197..3870 /note="pUC origin" vector join(2008..3873,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1999 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:29579 IMAGE:4871788" /tissue_type="Skin, melanotic melanoma, high MDR." /clone_lib="NIH_MGC_49" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..1999 /gene="FUCA1" /db_xref="GeneID:2517" /db_xref="HGNC:4006" /db_xref="MIM:230000" CDS 692..1999 /dnas_title="fucosidase, alpha-L- 1, tissue" /gene="FUCA1" /codon_start=1 /product="fucosidase, alpha-L- 1, tissue" /protein_id="AAH17338.1" /db_xref="GI:16878284" /db_xref="GeneID:2517" /db_xref="HGNC:4006" /db_xref="MIM:230000" /translation="MRSRPAGPALLLLLLFLGAAESVRRAQPPRRYTPDWPSLDSRPL PAWFDEAKFGVFIHWGVFSVPAWGSEWFWWHWQGEGRPQYQRFMRDNYPPGFSYADFG PQFTARFFHPEEWADLFQAAGAKYVVLTTKHHEGFTNWPSPVSWNWNSKDVGPHRDLV GELGTALRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVSAKTMPELYDLVNSYK PDLIWSDGEWECPDTYWNSTNFLSWLYNDSPVKDEVVVNDRWGQNCSCHHGGYYNCED KFKPQSLPDHKWEMCTSIDKFSWGYRRDMALSDVTEESEIISELVQTVSLGGNYLLNI GPTKDGLIVPIFQERLLAVGKWLSINGEAIYASKPWRVQWEKNTTSVWYTSKGSAVYA IFLHWPENGVLNLESPITTSTTKITMLGIQGDLKWSTDPDKGLFISLPQLPPSAVPAE FAWTIKLTGVK" misc_feature 692..1999 /note="FUCA1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccagcctccg cgccgctaca ccccagactg 721 gccgagcctg gattctcggc cgctgccggc ctggttcgac gaagccaagt tcggggtgtt 781 catccactgg ggcgtgttct cggtgcccgc ctggggcagc gagtggttct ggtggcactg 841 gcagggcgag gggcggccgc agtaccagcg cttcatgcgc gacaactacc cgcccggctt 901 cagctacgcc gacttcggac cgcagttcac tgcgcgcttc ttccacccgg aggagtgggc 961 cgacctcttc caggccgcgg gcgccaagta tgtagttttg acgacaaagc atcacgaagg 1021 cttcacaaac tggccgagtc ctgtgtcttg gaactggaac tccaaagacg tggggcctca 1081 tcgggatttg gttggtgaat tgggaacagc tctccggaag aggaacatcc gctatggact 1141 ataccactca ctcttagagt ggttccatcc actctatcta cttgataaga aaaatggctt 1201 caaaacacag cattttgtca gtgcaaaaac aatgccagag ctgtacgacc ttgttaacag 1261 ctataaacct gatctgatct ggtctgatgg ggagtgggaa tgtcctgata cttactggaa 1321 ctccacaaat tttctttcat ggctctacaa tgacagccct gtcaaggatg aggtggtagt 1381 aaatgaccga tggggtcaga actgttcctg tcaccatgga ggatactata actgtgaaga 1441 taaattcaag ccacagagct tgccagatca caagtgggag atgtgcacca gcattgacaa 1501 gttttcctgg ggctatcgtc gtgacatggc attgtctgat gttacagaag aatctgaaat 1561 catttcggaa ctggttcaga cagtaagttt gggaggcaac tatcttctga acattggacc 1621 aactaaagat ggactgattg ttcccatctt ccaagaaagg cttcttgctg ttgggaaatg 1681 gctgagcatc aatggggagg ctatctatgc ctccaaacca tggcgggtgc aatgggaaaa 1741 gaacacaaca tctgtatggt atacctcaaa gggatcggct gtttatgcca tttttctgca 1801 ctggccagaa aatggagtct taaaccttga atcccccata actacctcaa ctacaaagat 1861 aacaatgctg ggaattcaag gagatctgaa gtggtccaca gatccagata aaggtctctt 1921 catctctcta ccccagttgc caccctctgc tgtccccgca gagtttgctt ggactataaa 1981 gctgacagga gtgaagtaaC CAGCTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG 2041 CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA 2101 TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT 2161 CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAATAA TATCATCATG 2221 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA 2281 ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA 2341 ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC 2401 CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA 2461 TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT 2521 TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT 2581 CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT 2641 CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT 2701 TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA 2761 TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC 2821 ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA 2881 CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA 2941 GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT 3001 TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT 3061 CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT 3121 GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT 3181 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 3241 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 3301 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 3361 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 3421 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 3481 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 3541 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 3601 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 3661 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 3721 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 3781 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 3841 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT //