LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2012..2108) /label=attL2 /note="attL2 (c)" misc_signal complement(2123..2142) /note="T7 Promoter/priming site (c)" primer_bind 2150..2166 /note="M13 Reverse priming site" gene 2279..3088 /note="Kanamycin resistance gene" rep_origin 3209..3882 /note="pUC origin" vector join(2020..3885,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2011 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1314 IMAGE:3505533" /tissue_type="Placenta, choriocarcinoma" /clone_lib="NIH_MGC_21" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2011 /gene="FUCA2" /gene_synonym=dJ20N2.5 /gene_synonym=MGC1314 /gene_synonym=RP1-20N2.5 /db_xref="GeneID:2519" /db_xref="HGNC:4008" /db_xref="MIM:136820" CDS 692..2011 /dnas_title="fucosidase, alpha-L- 2, plasma" /gene="FUCA2" /gene_synonym=dJ20N2.5 /gene_synonym=MGC1314 /gene_synonym=RP1-20N2.5 /codon_start=1 /product="fucosidase, alpha-L- 2, plasma" /protein_id="AAH03060.1" /db_xref="GI:13111748" /db_xref="GeneID:2519" /db_xref="HGNC:4008" /db_xref="MIM:136820" /translation="MRPQELPRLAFPLLLLLLLLLPPPPCPAHSATRFDPTWESLDAR QLPAWFDQAKFGIFIHWGVFSVPSFGSEWFWWYWQKEKIPKYVEFMKDNYPPSFKYED FGPLFTAKFFNANQWADIFQASGAKYIVLTSKHHEGFTLWGSEYSWNWNAIDEGPKRD IVKELEVAIRNRTDLRFGLYYSLFEWFHPLFLEDESSSFHKRQFPVSKTLPELYELVN NYQPEVLWSDGDGGAPDQYWNSTGFLAWLYNESPVRGTVVTNDRWGAGSICKHGGFYT CSDRYNPGHLLPHKWENCMTIDKLSWGYRREAGISDYLTIEELVKQLVETVSCGGNLL MNIGPTLDGTISVVFEERLRQVGSWLKVNGEAIYETYTWRSQNDTVTPDVWYTSKPKE KLVYAIFLKWPTSGQLFLGHPKAILGATEVKLLGHGQPLNWISLEQNGIMVELPQLTI HQMPCKWGWALALTNVI" misc_feature 692..2011 /note="FUCA2 coding region" misc_difference 1673..1673 /gene="FUCA2" /gene_synonym=dJ20N2.5 /gene_synonym=MGC1314 /gene_synonym=RP1-20N2.5 /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'M' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 1718..1718 /gene="FUCA2" /gene_synonym=dJ20N2.5 /gene_synonym=MGC1314 /gene_synonym=RP1-20N2.5 /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'Y' in cDNA, 'H' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 1996..1996 /gene="FUCA2" /gene_synonym=dJ20N2.5 /gene_synonym=MGC1314 /gene_synonym=RP1-20N2.5 /note="'A' in cDNA is 'G' in the human genome; no amino acid change." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccacagcgcc acgcgcttcg accccacctg 721 ggagtccctg gacgcccgcc agctgcccgc gtggtttgac caggccaagt tcggcatctt 781 catccactgg ggagtgtttt ccgtgcccag cttcggtagc gagtggttct ggtggtattg 841 gcaaaaggaa aagataccga agtatgtgga atttatgaaa gataattacc ctcctagttt 901 caaatatgaa gattttggac cactatttac agcaaaattt tttaatgcca accagtgggc 961 agatattttt caggcctctg gtgccaaata cattgtctta acttccaaac atcatgaagg 1021 ctttaccttg tgggggtcag aatattcgtg gaactggaat gccatagatg aggggcccaa 1081 gagggacatt gtcaaggaac ttgaggtagc cattaggaac agaactgacc tgcgttttgg 1141 actgtactat tccctttttg aatggtttca tccgctcttc cttgaggatg aatccagttc 1201 attccataag cggcaatttc cagtttctaa gacattgcca gagctctatg agttagtgaa 1261 caactatcag cctgaggttc tgtggtcgga tggtgacgga ggagcaccgg atcaatactg 1321 gaacagcaca ggcttcttgg cctggttata taatgaaagc ccagttcggg gcacagtagt 1381 caccaatgat cgttggggag ctggtagcat ctgtaagcat ggtggcttct atacctgcag 1441 tgatcgttat aacccaggac atcttttgcc acataaatgg gaaaactgca tgacaataga 1501 caaactgtcc tggggctata ggagggaagc tggaatctct gactatctta caattgaaga 1561 attggtgaag caacttgtag agacagtttc atgtggagga aatcttttga tgaatattgg 1621 gcccacacta gatggcacca tttctgtagt ttttgaggag cgactgaggc aagtggggtc 1681 ctggctaaaa gtcaatggag aagctattta tgaaacctat acctggcgat cccagaatga 1741 cactgtcacc ccagatgtgt ggtacacatc caagcctaaa gaaaaattag tctatgccat 1801 ttttcttaaa tggcccacat caggacagct gttccttggc catcccaaag ctattctggg 1861 ggcaacagag gtgaaactac tgggccatgg acagccactt aactggattt ctttggagca 1921 aaatggcatt atggtagaac tgccacagct aaccattcat cagatgccgt gtaaatgggg 1981 ctgggctcta gccctaacta atgtgatcta aCCAGCTTTC TTGTACAAAG TTGGCATTAT 2041 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT 2101 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT 2161 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT 2221 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT 2281 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT 2341 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT 2401 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA 2461 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC 2521 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG 2581 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC 2641 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG 2701 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC 2761 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA 2821 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA 2881 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC 2941 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT 3001 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT 3061 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA 3121 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG 3181 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 3241 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 3301 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 3361 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 3421 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 3481 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 3541 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 3601 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 3661 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 3721 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 3781 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 3841 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT //