LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2303..2399) /label=attL2 /note="attL2 (c)" misc_signal complement(2414..2433) /note="T7 Promoter/priming site (c)" primer_bind 2441..2457 /note="M13 Reverse priming site" gene 2570..3379 /note="Kanamycin resistance gene" rep_origin 3500..4173 /note="pUC origin" PCR_frame 669..2303 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2323) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2320) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2311..4176,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2281..2301 /note="TEV site" source 673..2280 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:5191 IMAGE:2899915" /tissue_type="Placenta, choriocarcinoma" /clone_lib="NIH_MGC_10" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..2280 /gene="GBA" /gene_synonym=GBA1 /gene_synonym=GCB /db_xref="GeneID:2629" /db_xref="HGNC:4177" /db_xref="MIM:606463" CDS 673..2280 /dnas_title="glucosidase, beta; acid (includes glucosylceramidase)" /gene="GBA" /gene_synonym=GBA1 /gene_synonym=GCB /codon_start=1 /product="glucosidase, beta; acid (includes glucosylceramidase)" /protein_id="AAH03356.1" /db_xref="GI:13097171" /db_xref="GeneID:2629" /db_xref="HGNC:4177" /db_xref="MIM:606463" /translation="MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCI PKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGT GLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRV PMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPW TSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPS AGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVV LTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVR LGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTF YKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVP LTIKDPAVGFLETISPGYSIHTYLWRRQ" misc_feature 673..2280 /note="GBA coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggagtt ttcaagtcct tccagagagg aatgtcccaa gcctttgagt 721 agggtaagca tcatggctgg cagcctcaca ggattgcttc tacttcaggc agtgtcgtgg 781 gcatcaggtg cccgcccctg catccctaaa agcttcggct acagctcggt ggtgtgtgtc 841 tgcaatgcca catactgtga ctcctttgac cccccgacct ttcctgccct tggtaccttc 901 agccgctatg agagtacacg cagtgggcga cggatggagc tgagtatggg gcccatccag 961 gctaatcaca cgggcacagg cctgctactg accctgcagc cagaacagaa gttccagaaa 1021 gtgaagggat ttggaggggc catgacagat gctgctgctc tcaacatcct tgccctgtca 1081 ccccctgccc aaaatttgct acttaaatcg tacttctctg aagaaggaat cggatataac 1141 atcatccggg tacccatggc cagctgtgac ttctccatcc gcacctacac ctatgcagac 1201 acccctgatg atttccagtt gcacaacttc agcctcccag aggaagatac caagctcaag 1261 atacccctga ttcaccgagc cctgcagttg gcccagcgtc ccgtttcact ccttgccagc 1321 ccctggacat cacccacttg gctcaagacc aatggagcgg tgaatgggaa ggggtcactc 1381 aagggacagc ccggagacat ctaccaccag acctgggcca gatactttgt gaagttcctg 1441 gatgcctatg ctgagcacaa gttacagttc tgggcagtga cagctgaaaa tgagccttct 1501 gctgggctgt tgagtggata ccccttccag tgcctgggct tcacccctga acatcagcga 1561 gacttcattg cccgtgacct aggtcctacc ctcgccaaca gtactcacca caatgtccgc 1621 ctactcatgc tggatgacca acgcttgctg ctgccccact gggcaaaggt ggtactgaca 1681 gacccagaag cagctaaata tgttcatggc attgctgtac attggtacct ggactttctg 1741 gctccagcca aagccaccct aggggagaca caccgcctgt tccccaacac catgctcttt 1801 gcctcagagg cctgtgtggg ctccaagttc tgggagcaga gtgtgcggct aggctcctgg 1861 gatcgaggga tgcagtacag ccacagcatc atcacgaacc tcctgtacca tgtggtcggc 1921 tggaccgact ggaaccttgc cctgaacccc gaaggaggac ccaattgggt gcgtaacttt 1981 gtcgacagtc ccatcattgt agacatcacc aaggacacgt tttacaaaca gcccatgttc 2041 taccaccttg gccacttcag caagttcatt cctgagggct cccagagagt ggggctggtt 2101 gccagtcaga agaacgacct ggacgcagtg gcactgatgc atcccgatgg ctctgctgtt 2161 gtggtcgtgc taaaccgctc ctctaaggat gtgcctctta ccatcaagga tcctgctgtg 2221 ggcttcctgg agacaatctc acctggctac tccattcaca cctacctgtg gcgtcgccag 2281 gaaaacttgt actttcaagg ctCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 2341 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG 2401 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA 2461 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC 2521 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT 2581 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA 2641 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA 2701 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC 2761 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA 2821 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC 2881 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT 2941 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT 3001 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT 3061 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT 3121 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT 3181 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA 3241 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG 3301 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT 3361 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC 3421 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC 3481 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 3541 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 3601 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 3661 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 3721 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 3781 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 3841 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 3901 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 3961 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 4021 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 4081 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 4141 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT //