LOCUS GBA-pGEc2-DEST 6829 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6307..6436) /note="3'-ITR" misc_feature complement(6005..6219) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5457..5998 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4636..4658 /note="attB2" misc_feature 5436..5441 /note="BstB1 site" misc_feature 4659..5354 /note="Super GFP" misc_feature 5355..5405 /note="AviTag" misc_feature 5406..5429 /note="His Tag" PCR_frame 3002..4636 /note="Reading Frame" /set="Set 1" source 3006..4613 /dnas_title="GBA-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:5191 IMAGE:2899915" /tissue_type="Placenta, choriocarcinoma" /clone_lib="NIH_MGC_10" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4613 /dnas_title="GBA" /gene="GBA" /gene_synonym="GBA1" /gene_synonym="GCB" /db_xref="GeneID:2629" /db_xref="HGNC:4177" /db_xref="MIM:606463" CDS 3006..4613 /dnas_title="GBA" /gene="GBA" /gene_synonym="GBA1" /gene_synonym="GCB" /codon_start=1 /product="glucosidase, beta; acid (includes glucosylceramidase)" /protein_id="AAH03356.1" /db_xref="GI:13097171" /db_xref="GeneID:2629" /db_xref="HGNC:4177" /db_xref="MIM:606463" /translation="MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCI PKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGT GLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRV PMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPW TSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPS AGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVV LTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVR LGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTF YKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVP LTIKDPAVGFLETISPGYSIHTYLWRRQ" misc_feature 3006..4613 /note="GBA coding region" misc_feature 4614..4634 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4643) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4643) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4644..6829,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4643 /source="GBA-Cterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgga gttttcaagt ccttccagag aggaatgtcc caagcctttg agtagggtaa 3061 gcatcatggc tggcagcctc acaggattgc ttctacttca ggcagtgtcg tgggcatcag 3121 gtgcccgccc ctgcatccct aaaagcttcg gctacagctc ggtggtgtgt gtctgcaatg 3181 ccacatactg tgactccttt gaccccccga cctttcctgc ccttggtacc ttcagccgct 3241 atgagagtac acgcagtggg cgacggatgg agctgagtat ggggcccatc caggctaatc 3301 acacgggcac aggcctgcta ctgaccctgc agccagaaca gaagttccag aaagtgaagg 3361 gatttggagg ggccatgaca gatgctgctg ctctcaacat ccttgccctg tcaccccctg 3421 cccaaaattt gctacttaaa tcgtacttct ctgaagaagg aatcggatat aacatcatcc 3481 gggtacccat ggccagctgt gacttctcca tccgcaccta cacctatgca gacacccctg 3541 atgatttcca gttgcacaac ttcagcctcc cagaggaaga taccaagctc aagatacccc 3601 tgattcaccg agccctgcag ttggcccagc gtcccgtttc actccttgcc agcccctgga 3661 catcacccac ttggctcaag accaatggag cggtgaatgg gaaggggtca ctcaagggac 3721 agcccggaga catctaccac cagacctggg ccagatactt tgtgaagttc ctggatgcct 3781 atgctgagca caagttacag ttctgggcag tgacagctga aaatgagcct tctgctgggc 3841 tgttgagtgg ataccccttc cagtgcctgg gcttcacccc tgaacatcag cgagacttca 3901 ttgcccgtga cctaggtcct accctcgcca acagtactca ccacaatgtc cgcctactca 3961 tgctggatga ccaacgcttg ctgctgcccc actgggcaaa ggtggtactg acagacccag 4021 aagcagctaa atatgttcat ggcattgctg tacattggta cctggacttt ctggctccag 4081 ccaaagccac cctaggggag acacaccgcc tgttccccaa caccatgctc tttgcctcag 4141 aggcctgtgt gggctccaag ttctgggagc agagtgtgcg gctaggctcc tgggatcgag 4201 ggatgcagta cagccacagc atcatcacga acctcctgta ccatgtggtc ggctggaccg 4261 actggaacct tgccctgaac cccgaaggag gacccaattg ggtgcgtaac tttgtcgaca 4321 gtcccatcat tgtagacatc accaaggaca cgttttacaa acagcccatg ttctaccacc 4381 ttggccactt cagcaagttc attcctgagg gctcccagag agtggggctg gttgccagtc 4441 agaagaacga cctggacgca gtggcactga tgcatcccga tggctctgct gttgtggtcg 4501 tgctaaaccg ctcctctaag gatgtgcctc ttaccatcaa ggatcctgct gtgggcttcc 4561 tggagacaat ctcacctggc tactccattc acacctacct gtggcgtcgc caggaaaact 4621 tgtactttca aggctCCAGC TTTCTTGTAC AAAGTGGTAT GAGCAAGGGC GAGGAACTGT 4681 TCACCGGCGT GGTGCCCATC CTGGTGGAAC TGGATGGCGA CGTGAACGGC CACAAGTTCT 4741 CTGTGCGGGG AGAGGGCGAA GGCGACGCCA CAAATGGCAA GCTGACCCTG AAGTTCATCT 4801 GCACCACCGG CAAGCTGCCC GTGCCTTGGC CTACCCTCGT GACCACACTG ACCTACGGCG 4861 TGCAGTGCTT CAGCAGATAC CCCGACCATA TGAAGCGGCA CGACTTCTTC AAGAGCGCCA 4921 TGCCCGAGGG CTACGTGCAG GAACGGACCA TCAGCTTCAA GGACGACGGC ACCTACAAGA 4981 CCAGAGCCGA AGTGAAGTTC GAGGGCGACA CCCTCGTGAA CCGGATCGAG CTGAAGGGCA 5041 TCGATTTCAA AGAGGACGGC AACATCCTGG GCCACAAGCT GGAGTACAAC TTCAACAGCC 5101 ACAACGTGTA CATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC TTCAAGATCC 5161 GGCACAACGT GGAAGATGGC AGCGTGCAGC TGGCCGACCA CTACCAGCAG AACACCCCCA 5221 TCGGAGATGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG AGCGTGCTGA 5281 GCAAGGACCC CAACGAGAAG CGGGACCACA TGGTGCTGCT GGAATTCGTG ACCGCCGCTG 5341 GCATCACCCA CGGCATGAGC GGCCTGAACG ACATCTTCGA GGCCCAGAAA ATCGAGTGGC 5401 ACGAGCACCA CCATCATCAC CATCACCACT GATGATTCGA AAAATAATAA GGATCCAATC 5461 AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT 5521 TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG 5581 CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC 5641 CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT 5701 GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG 5761 CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG 5821 GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT 5881 GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC 5941 CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGAG 6001 ATCTGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC 6061 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC 6121 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA 6181 GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAC TCGAGTTAAG GGCGAATTGC 6241 CGATTAGGAT CTTCCTAGAG CATGGCTACG TAGATAAGTA GCATGGCGGG TTAATCATTA 6301 ACTACAAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC TCTGCGCGCT CGCTCGCTCA 6361 CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA 6421 GCGAGCGAGC GCGCAGCCTT AATTAAGAGC TCATGGCGCG CCTAGGCCTT GACGGCCTTC 6481 CGCCAATTCG CCCTATAGTG AGTCGTATTA CGTCGCGCTC ACTGGCCGTC GTTTTACAAC 6541 GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT 6601 TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGAAAC GCCCTTCCCA ACAGTTGCGC 6661 AGCCTGAATG GCGAATGGGA GCGCCCTGTA GCGGCCACTC AACCCTATCT CGGTCTATTC 6721 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA 6781 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAG //