LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2186..2282) /label=attL2 /note="attL2 (c)" misc_signal complement(2297..2316) /note="T7 Promoter/priming site (c)" primer_bind 2324..2340 /note="M13 Reverse priming site" gene 2453..3262 /note="Kanamycin resistance gene" rep_origin 3383..4056 /note="pUC origin" vector join(2194..4059,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2185 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:5191 IMAGE:2899915" /tissue_type="Placenta, choriocarcinoma" /clone_lib="NIH_MGC_10" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..2185 /gene="GBA" /gene_synonym=GBA1 /gene_synonym=GCB /db_xref="GeneID:2629" /db_xref="HGNC:4177" /db_xref="MIM:606463" CDS 692..2185 /dnas_title="glucosidase, beta; acid (includes glucosylceramidase)" /gene="GBA" /gene_synonym=GBA1 /gene_synonym=GCB /codon_start=1 /product="glucosidase, beta; acid (includes glucosylceramidase)" /protein_id="AAH03356.1" /db_xref="GI:13097171" /db_xref="GeneID:2629" /db_xref="HGNC:4177" /db_xref="MIM:606463" /translation="MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCI PKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGT GLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRV PMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPW TSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPS AGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVV LTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVR LGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTF YKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVP LTIKDPAVGFLETISPGYSIHTYLWRRQ" misc_feature 692..2185 /note="GBA coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgcccgcccc tgcatcccta aaagcttcgg 721 ctacagctcg gtggtgtgtg tctgcaatgc cacatactgt gactcctttg accccccgac 781 ctttcctgcc cttggtacct tcagccgcta tgagagtaca cgcagtgggc gacggatgga 841 gctgagtatg gggcccatcc aggctaatca cacgggcaca ggcctgctac tgaccctgca 901 gccagaacag aagttccaga aagtgaaggg atttggaggg gccatgacag atgctgctgc 961 tctcaacatc cttgccctgt caccccctgc ccaaaatttg ctacttaaat cgtacttctc 1021 tgaagaagga atcggatata acatcatccg ggtacccatg gccagctgtg acttctccat 1081 ccgcacctac acctatgcag acacccctga tgatttccag ttgcacaact tcagcctccc 1141 agaggaagat accaagctca agatacccct gattcaccga gccctgcagt tggcccagcg 1201 tcccgtttca ctccttgcca gcccctggac atcacccact tggctcaaga ccaatggagc 1261 ggtgaatggg aaggggtcac tcaagggaca gcccggagac atctaccacc agacctgggc 1321 cagatacttt gtgaagttcc tggatgccta tgctgagcac aagttacagt tctgggcagt 1381 gacagctgaa aatgagcctt ctgctgggct gttgagtgga taccccttcc agtgcctggg 1441 cttcacccct gaacatcagc gagacttcat tgcccgtgac ctaggtccta ccctcgccaa 1501 cagtactcac cacaatgtcc gcctactcat gctggatgac caacgcttgc tgctgcccca 1561 ctgggcaaag gtggtactga cagacccaga agcagctaaa tatgttcatg gcattgctgt 1621 acattggtac ctggactttc tggctccagc caaagccacc ctaggggaga cacaccgcct 1681 gttccccaac accatgctct ttgcctcaga ggcctgtgtg ggctccaagt tctgggagca 1741 gagtgtgcgg ctaggctcct gggatcgagg gatgcagtac agccacagca tcatcacgaa 1801 cctcctgtac catgtggtcg gctggaccga ctggaacctt gccctgaacc ccgaaggagg 1861 acccaattgg gtgcgtaact ttgtcgacag tcccatcatt gtagacatca ccaaggacac 1921 gttttacaaa cagcccatgt tctaccacct tggccacttc agcaagttca ttcctgaggg 1981 ctcccagaga gtggggctgg ttgccagtca gaagaacgac ctggacgcag tggcactgat 2041 gcatcccgat ggctctgctg ttgtggtcgt gctaaaccgc tcctctaagg atgtgcctct 2101 taccatcaag gatcctgctg tgggcttcct ggagacaatc tcacctggct actccattca 2161 cacctacctg tggcgtcgcc agtgaCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 2221 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 2281 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 2341 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 2401 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 2461 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 2521 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 2581 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 2641 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 2701 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 2761 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 2821 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 2881 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 2941 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 3001 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 3061 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 3121 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 3181 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 3241 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 3301 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 3361 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 3421 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 3481 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 3541 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 3601 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 3661 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 3721 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 3781 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 3841 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 3901 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 3961 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 4021 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //