LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3470..3566) /label=attL2 /note="attL2 (c)" misc_signal complement(3581..3600) /note="T7 Promoter/priming site (c)" primer_bind 3608..3624 /note="M13 Reverse priming site" gene 3737..4546 /note="Kanamycin resistance gene" rep_origin 4667..5340 /note="pUC origin" vector join(3478..5343,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..3469 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:48746 IMAGE:5739991" /tissue_type="Duodenum, adenocarcinoma" /clone_lib="NIH_MGC_88" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..3469 /gene="GAA" /gene_synonym=LYAG /db_xref="GeneID:2548" /db_xref="HGNC:4065" /db_xref="MIM:606800" CDS 692..3469 /dnas_title="glucosidase, alpha; acid" /gene="GAA" /gene_synonym=LYAG /codon_start=1 /product="glucosidase, alpha; acid" /protein_id="AAH40431.1" /db_xref="GI:26251857" /db_xref="GeneID:2548" /db_xref="HGNC:4065" /db_xref="MIM:606800" /translation="MGVRHPPCSHRLLAVCALVSLATAALLGHILLHDFLLVPRELSG SSPVLEETHPAHQQGASRPGPRDAQAHPGRPRAVPTQCDVPPNSRFDCAPDKAITQEQ CEARGCCYIPAKQGLQGAQMGQPWCFFPPSYPSYKLENLSSSEMGYTATLTRTTPTFF PKDILTLRLDVMMETENRLHFTIKDPANRRYEVPLETPHVHSRAPSPLYSVEFSEEPF GVIVRRQLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYITGLAEHLSPLMLSTSWTRI TLWNRDLAPTPGANLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTG GILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAITRQVVENMT RAHFPLDVQWNDLDYMDSRRDFTFNKDGFRDFPAMVQELHQGGRRYMMIVDPAISSSG PAGSYRPYDEGLRRGVFITNETGQPLIGKVWPGSTAFPDFTNPTALAWWEDMVAEFHD QVPFDGMWIDMNEPSNFIRGSEDGCPNNELENPPYVPGVVGGTLQAATICASSHQFLS THYNLHNLYGLTEAIASHRALVKARGTRPFVISRSTFAGHGRYAGHWTGDVWSSWEQL ASSVPEILQFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNSLLSLPQ EPYSFSEPAQQAMRKALTLRYALLPHLYTLFHQAHVAGETVARPLFLEFPKDSSTWTV DHQLLWGEALLITPVLQAGKAEVTGYFPLGTWYDLQTVPVEALGSLPPPPAAPREPAI HSEGQWVTLPAPLDTINVHLRAGYIIPLQGPGLTTTESRQQPMALAVALTKGGEARGE LFWDDGESLEVLERGAYTQVIFLARNNTIVNELVRVTSEGAGLQLQKVTVLGVATAPQ QVLSNGVPVSNFTYSPDTKVLDICVSLLMGEQFLVSWC" misc_difference 793..793 /gene="GAA" /gene_synonym=LYAG /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 692..3469 /note="GAA coding region" misc_difference 3181..3181 /gene="GAA" /gene_synonym=LYAG /note="'T' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgggcacatc ctactccatg atttcctgct 721 ggttccccga gagctgagtg gctcctcccc agtcctggag gagactcacc cagctcacca 781 gcagggagcc agtagaccag ggccccggga tgcccaggca caccccggcc gtcccagagc 841 agtgcccaca cagtgcgacg tcccccccaa cagccgcttc gattgcgccc ctgacaaggc 901 catcacccag gaacagtgcg aggcccgcgg ctgttgctac atccctgcaa agcaggggct 961 gcagggagcc cagatggggc agccctggtg cttcttccca cccagctacc ccagctacaa 1021 gctggagaac ctgagctcct ctgaaatggg ctacacggcc accctgaccc gtaccacccc 1081 caccttcttc cccaaggaca tcctgaccct gcggctggac gtgatgatgg agactgagaa 1141 ccgcctccac ttcacgatca aagatccagc taacaggcgc tacgaggtgc ccttggagac 1201 cccgcatgtc cacagccggg caccgtcccc actctacagc gtggagttct ccgaggagcc 1261 cttcggggtg atcgtgcgcc ggcagctgga cggccgcgtg ctgctgaaca cgacggtggc 1321 gcccctgttc tttgcggacc agttccttca gctgtccacc tcgctgccct cgcagtatat 1381 cacaggcctc gccgagcacc tcagtcccct gatgctcagc accagctgga ccaggatcac 1441 cctgtggaac cgggaccttg cgcccacgcc cggtgcgaac ctctacgggt ctcacccttt 1501 ctacctggcg ctggaggacg gcgggtcggc acacggggtg ttcctgctaa acagcaatgc 1561 catggatgtg gtcctgcagc cgagccctgc ccttagctgg aggtcgacag gtgggatcct 1621 ggatgtctac atcttcctgg gcccagagcc caagagcgtg gtgcagcagt acctggacgt 1681 tgtgggatac ccgttcatgc cgccatactg gggcctgggc ttccacctgt gccgctgggg 1741 ctactcctcc accgctatca cccgccaggt ggtggagaac atgaccaggg cccacttccc 1801 cctggacgtc cagtggaacg acctggacta catggactcc cggagggact tcacgttcaa 1861 caaggatggc ttccgggact tcccggccat ggtgcaggag ctgcaccagg gcggccggcg 1921 ctacatgatg atcgtggatc ctgccatcag cagctcgggc cctgccggga gctacaggcc 1981 ctacgacgag ggtctgcgga ggggggtttt catcaccaac gagaccggcc agccgctgat 2041 tgggaaggta tggcccgggt ccactgcctt ccccgacttc accaacccca cagccctggc 2101 ctggtgggag gacatggtgg ctgagttcca tgaccaggtg cccttcgacg gcatgtggat 2161 tgacatgaac gagccttcca acttcatcag gggctctgag gacggctgcc ccaacaatga 2221 gctggagaac ccaccctacg tgcctggggt ggttgggggg accctccagg cggccaccat 2281 ctgtgcctcc agccaccagt ttctctccac acactacaac ctgcacaacc tctacggcct 2341 gaccgaagcc atcgcctccc acagggcgct ggtgaaggct cgggggacac gcccatttgt 2401 gatctcccgc tcgacctttg ctggccacgg ccgatacgcc ggccactgga cgggggacgt 2461 gtggagctcc tgggagcagc tcgcctcctc cgtgccagaa atcctgcagt ttaacctgct 2521 gggggtgcct ctggtcgggg ccgacgtctg cggcttcctg ggcaacacct cagaggagct 2581 gtgtgtgcgc tggacccagc tgggggcctt ctaccccttc atgcggaacc acaacagcct 2641 gctcagtctg ccccaggagc cgtacagctt cagcgagccg gcccagcagg ccatgaggaa 2701 ggccctcacc ctgcgctacg cactcctccc ccacctctac acactgttcc accaggccca 2761 cgtcgcgggg gagaccgtgg cccggcccct cttcctggag ttccccaagg actctagcac 2821 ctggactgtg gaccaccagc tcctgtgggg ggaggccctg ctcatcaccc cagtgctcca 2881 ggccgggaag gccgaagtga ctggctactt ccccttgggc acatggtacg acctgcagac 2941 ggtgccagta gaggcccttg gcagcctccc acccccacct gcagctcccc gtgagccagc 3001 catccacagc gaggggcagt gggtgacgct gccggccccc ctggacacca tcaacgtcca 3061 cctccgggct gggtacatca tccccctgca gggccctggc ctcacaacca cagagtcccg 3121 ccagcagccc atggccctgg ctgtggccct gaccaagggt ggggaggccc gaggggagct 3181 tttctgggac gatggagaga gcctggaagt gctggagcga ggggcctaca cacaggtcat 3241 cttcctggcc aggaataaca cgatcgtgaa tgagctggta cgtgtgacca gtgagggagc 3301 tggcctgcag ctgcagaagg tgactgtcct gggcgtggcc acggcgcccc agcaggtcct 3361 ctccaacggt gtccctgtct ccaacttcac ctacagcccc gacaccaagg tcctggacat 3421 ctgtgtctcg ctgttgatgg gagagcagtt tctcgtcagc tggtgttagC CAGCTTTCTT 3481 GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC 3541 TATCAGTCAA AATAAAATCA TTATTTGCCA TCCAGCTGAT ATCCCCTATA GTGAGTCGTA 3601 TTACATGGTC ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG TCTCAAAATC TCTGATGTTA 3661 CATTGCACAA GATAAAATAA TATCATCATG AACAATAAAA CTGTCTGCTT ACATAAACAG 3721 TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC 3781 CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG 3841 TGCGACAATC TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG 3901 CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA 3961 ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT 4021 CACCACTGCG ATCCCCGGAA AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG 4081 TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG 4141 TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA 4201 TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA 4261 AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG 4321 TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT 4381 TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG 4441 TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA 4501 TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA 4561 TTGGTTGTAA CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC 4621 AAAATCCCTT AACGTGAGTT ACGCGTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT 4681 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA 4741 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA 4801 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT 4861 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT 4921 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA 4981 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT 5041 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC 5101 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA 5161 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG 5221 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA 5281 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT 5341 GTT //