LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3446..3542) /label=attL2 /note="attL2 (c)" misc_signal complement(3557..3576) /note="T7 Promoter/priming site (c)" primer_bind 3584..3600 /note="M13 Reverse priming site" gene 3713..4522 /note="Kanamycin resistance gene" rep_origin 4643..5316 /note="pUC origin" vector join(3454..5319,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..3439 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /sex="male" /tissue_type="Brain" /lab_host="Escherichia coli XL1-Blue" /plasmid="pACT2" gene 692..3439 /gene="KIAA0088" CDS 692..3439 /dnas_title="Glucosidase II" /gene="KIAA0088" /function="Glucose trimming of glycosylated proteins within the endoplasmic reticulum" /experiment="experimental evidence, no additional details recorded" /codon_start=1 /product="Glucosidase II" /protein_id="CAA04006.1" /db_xref="GI:2274968" /db_xref="GOA:Q14697" /db_xref="HGNC:4138" /db_xref="InterPro:IPR000322" /db_xref="UniProtKB/Swiss-Prot:Q14697" /translation="MAAVAAVAARRRRSWASLVLAFLGVCLGITLAVDRSNFKTCEES SFCKRQRSIRPGLSPYRALLDSLQLGPDSLTVHLIHEVTKVLLVLELQGLQKNMTRFR IDELEPRRPRYRVPDVLVADPPIARLSVSGRDENSVELTMAEGPYKIILTARPFRLDL LEDRSLLLSVNARGLLEFEHQRAPRVSQGSKDPAEGDGAQPEETPRDGDKPEETQGKA EKDEPGAWEETFKTHSDSKPYGPMSVGLDFSLPGMEHVYGIPEHADNLRLKVTEGGEP YRLYNLDVFQYELYNPMALYGSVPVLLAHNPHRDLGIFWLNAAETWVDISSNTAGKTL FGKMMDYLQGSGETPQTDVRWMSETGIIDVFLLLGPSISDVFRQYASLTGTQALPPLF SLGYHQSRWNYRDEADVLEVDQGFDDHNLPCDVIWLDIEHADGKRYFTWDPSRFPQPR TMLERLASKRRKLVAIVDPHIKVDSGYRVHEELRNLGLYVKTRDGSDYEGWCWPGSAG YPDFTNPTMRAWWANMFSYDNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAQHYGGW EHRDVHNIYGLYVHMATADGLRQRSGGMERPFVLARAFFAGSQRFGAVWTGDNTAEWD HLKISIPMCLSLGLVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTG RREPWLLPSQHNDIIRDALGQRYSLLPFWYTLLYQAHREGIPVMRPLWVQYPQDVTTF NIDDQYLLGDALLVHPVSDSGAHGVQVYLPGQGEVWYDIQSYQKHHGPQTLYLPVTLS SIPVFQRGGTIVPRWMRVRRSSECMKDDPITLFVALSPQGTAQGELFLDDGYTFNYQT RQEFLLRRFSFSGNTLVSSSADPEGHFETPIWIERVVIIGAGKPAAVVLQTKGSPESR LSFQHDPETSVLVLRKPGINVASDWSIHLR" sig_peptide 692..703 /gene="KIAA0088" mat_peptide 704..3439 /dnas_title="Glucosidase II" /gene="KIAA0088" /product="Glucosidase II" /function="Glucose trimming of glycosylated proteins within the endoplasmic reticulum" misc_feature 692..3439 /note="GANAB coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cattaccctt gctgtggata gaagcaactt 721 taagacctgt gaagagagtt ctttctgcaa gcgacagaga agcatacggc caggcctctc 781 tccataccga gccttgctgg actctctaca gcttggtcct gattccctca cggtccatct 841 gatccatgag gtcaccaagg tgttgctggt gctagagctt caggggcttc aaaagaacat 901 gactcggttc aggattgatg agctggagcc tcggcgaccc cgataccgtg taccagatgt 961 tttggtggct gatccaccaa tagcccggct ttctgtctct ggtcgtgatg agaacagtgt 1021 ggagttaacc atggctgagg gaccctacaa gatcatcttg acagcacggc cattccgcct 1081 tgacctacta gaggaccgaa gtcttttgct tagtgtcaat gcccgaggac tcttggagtt 1141 tgagcatcag agggccccta gggtctcgca aggatcaaaa gacccagctg agggcgatgg 1201 ggcccagcct gaggaaacac ccagggatgg cgacaagcca gaggagactc aggggaaggc 1261 agagaaagat gagccaggag cctgggagga gacattcaaa actcactctg acagcaagcc 1321 gtatggcccc atgtctgtgg gtttggactt ctctctgcca ggcatggagc atgtctatgg 1381 gatccctgag catgcagaca acctgaggct gaaggtcact gagggtgggg agccatatcg 1441 cctctacaat ttggatgtgt tccagtatga gctgtacaac ccaatggcct tgtatgggtc 1501 tgtgcctgtg ctcctggcac acaaccctca tcgcgacttg ggcatcttct ggctcaatgc 1561 tgcagagacc tgggttgata tatcttccaa cactgccggg aagaccctgt ttgggaagat 1621 gatggactac ctgcagggct ctggggagac cccacagaca gatgttcgct ggatgtcaga 1681 gactggcatc attgacgtct tcctgctgct ggggccctcc atctctgatg ttttccggca 1741 atatgctagt ctcacaggaa cccaggcgtt gcccccactc ttctccctcg gctaccacca 1801 gagccgttgg aactaccggg acgaggctga tgtgctggaa gtggatcagg gctttgatga 1861 tcacaacctg ccctgtgatg tcatctggct agacattgaa catgctgatg gcaagcggta 1921 tttcacctgg gaccccagtc gcttccctca gccccgcacc atgcttgagc gcttggcttc 1981 taagaggcgg aagctggtgg ccatcgtaga cccccacatc aaggtggact ccggctaccg 2041 agttcacgag gagctgcgga acctggggct gtatgttaaa acccgggatg gctctgacta 2101 tgagggctgg tgctggccag gctcagctgg ttaccctgac ttcactaatc ccacgatgag 2161 ggcctggtgg gctaacatgt tcagctatga caattatgag ggctcagctc ccaacctctt 2221 tgtctggaat gacatgaacg aaccatctgt gttcaatggt cctgaggtca ccatgctcaa 2281 ggatgcccag cattatgggg gctgggagca ccgggatgtg cataacatct atggccttta 2341 tgtgcacatg gcgactgctg atgggctgag acagcgctct gggggcatgg aacgcccctt 2401 tgtcctggcc agggccttct tcgctggctc ccagcgcttt ggagccgtgt ggacagggga 2461 caacactgcc gagtgggacc atttgaagat ctctattcct atgtgtctca gcttggggct 2521 ggtgggactt tccttctgtg gggcggatgt gggtggcttc ttcaaaaacc cagagccaga 2581 gctgcttgtg cgctggtacc agatgggtgc ttaccagcca ttcttccggg cacatgccca 2641 cttggacact gggcgacgag agccatggct gttaccatct cagcacaatg atataatccg 2701 agatgccttg ggccagcgat attctttgct gcccttctgg tacaccctct tatatcaggc 2761 ccatcgggaa ggcattcctg tcatgaggcc cctgtgggtg cagtaccctc aggatgtgac 2821 taccttcaat atagatgatc agtacttgct tggggatgcg ttgctggttc accctgtatc 2881 agactctgga gcccatggtg tccaggtcta tctgcctggc caaggggagg tgtggtatga 2941 cattcaaagc taccagaagc atcatggtcc ccagaccctg tacctgcctg taactctaag 3001 cagtatccct gtgttccagc gtggagggac aatcgtgcct cgatggatgc gagtgcggcg 3061 gtcttcagaa tgtatgaagg atgaccccat cactctcttt gttgcactta gccctcaggg 3121 tacagctcaa ggagagctct ttctggatga tgggtacacg ttcaactatc agactcgcca 3181 agagttcctg ctgcgtcgat tctcattctc tggcaacacc cttgtctcca gctcagcaga 3241 ccctgaagga cactttgaga caccaatctg gattgagcgg gtggtgataa taggggctgg 3301 aaagccagca gctgtggtac tccagacaaa aggatctcca gaaagccgcc tgtccttcca 3361 gcatgaccct gagacctctg tgttggtcct gcgcaagcct ggcatcaatg tggcatctga 3421 ttggagtatt cacctgcgaT AGGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 3481 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 3541 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 3601 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 3661 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 3721 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 3781 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 3841 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 3901 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 3961 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 4021 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 4081 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 4141 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 4201 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 4261 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 4321 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 4381 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 4441 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 4501 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 4561 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 4621 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 4681 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 4741 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 4801 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 4861 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 4921 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 4981 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 5041 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 5101 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 5161 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 5221 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 5281 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //