LOCUS GANAB-pGEn1-DEST 7330 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 5958..6499 /note="WPRE" misc_recomb 5920..5942 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(6506..6720) /note="BGH pA" misc_feature complement(6808..6937) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..5913 /dnas_title="GANAB-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /sex="male" /tissue_type="Brain" /lab_host="Escherichia coli XL1-Blue" /plasmid="pACT2" gene 3166..5913 /dnas_title="KIAA0088" /gene="KIAA0088" CDS 3166..5913 /dnas_title="Glucosidase II" /gene="KIAA0088" /function="Glucose trimming of glycosylated proteins within the endoplasmic reticulum" /experiment="experimental evidence, no additional details recorded" /codon_start=1 /product="Glucosidase II" /protein_id="CAA04006.1" /db_xref="GI:2274968" /db_xref="GOA:Q14697" /db_xref="HGNC:4138" /db_xref="InterPro:IPR000322" /db_xref="UniProtKB/Swiss-Prot:Q14697" /translation="MAAVAAVAARRRRSWASLVLAFLGVCLGITLAVDRSNFKTCEES SFCKRQRSIRPGLSPYRALLDSLQLGPDSLTVHLIHEVTKVLLVLELQGLQKNMTRFR IDELEPRRPRYRVPDVLVADPPIARLSVSGRDENSVELTMAEGPYKIILTARPFRLDL LEDRSLLLSVNARGLLEFEHQRAPRVSQGSKDPAEGDGAQPEETPRDGDKPEETQGKA EKDEPGAWEETFKTHSDSKPYGPMSVGLDFSLPGMEHVYGIPEHADNLRLKVTEGGEP YRLYNLDVFQYELYNPMALYGSVPVLLAHNPHRDLGIFWLNAAETWVDISSNTAGKTL FGKMMDYLQGSGETPQTDVRWMSETGIIDVFLLLGPSISDVFRQYASLTGTQALPPLF SLGYHQSRWNYRDEADVLEVDQGFDDHNLPCDVIWLDIEHADGKRYFTWDPSRFPQPR TMLERLASKRRKLVAIVDPHIKVDSGYRVHEELRNLGLYVKTRDGSDYEGWCWPGSAG YPDFTNPTMRAWWANMFSYDNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAQHYGGW EHRDVHNIYGLYVHMATADGLRQRSGGMERPFVLARAFFAGSQRFGAVWTGDNTAEWD HLKISIPMCLSLGLVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTG RREPWLLPSQHNDIIRDALGQRYSLLPFWYTLLYQAHREGIPVMRPLWVQYPQDVTTF NIDDQYLLGDALLVHPVSDSGAHGVQVYLPGQGEVWYDIQSYQKHHGPQTLYLPVTLS SIPVFQRGGTIVPRWMRVRRSSECMKDDPITLFVALSPQGTAQGELFLDDGYTFNYQT RQEFLLRRFSFSGNTLVSSSADPEGHFETPIWIERVVIIGAGKPAAVVLQTKGSPESR LSFQHDPETSVLVLRKPGINVASDWSIHLR" sig_peptide 3166..3177 /dnas_title="KIAA0088" /gene="KIAA0088" mat_peptide 3178..5913 /dnas_title="Glucosidase II" /gene="KIAA0088" /product="Glucosidase II" /function="Glucose trimming of glycosylated proteins within the endoplasmic reticulum" misc_feature 3166..5913 /note="GANAB coding region" vector join(5928..7330,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..5927 /source="GANAB-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcattac ccttgctgtg 3181 gatagaagca actttaagac ctgtgaagag agttctttct gcaagcgaca gagaagcata 3241 cggccaggcc tctctccata ccgagccttg ctggactctc tacagcttgg tcctgattcc 3301 ctcacggtcc atctgatcca tgaggtcacc aaggtgttgc tggtgctaga gcttcagggg 3361 cttcaaaaga acatgactcg gttcaggatt gatgagctgg agcctcggcg accccgatac 3421 cgtgtaccag atgttttggt ggctgatcca ccaatagccc ggctttctgt ctctggtcgt 3481 gatgagaaca gtgtggagtt aaccatggct gagggaccct acaagatcat cttgacagca 3541 cggccattcc gccttgacct actagaggac cgaagtcttt tgcttagtgt caatgcccga 3601 ggactcttgg agtttgagca tcagagggcc cctagggtct cgcaaggatc aaaagaccca 3661 gctgagggcg atggggccca gcctgaggaa acacccaggg atggcgacaa gccagaggag 3721 actcagggga aggcagagaa agatgagcca ggagcctggg aggagacatt caaaactcac 3781 tctgacagca agccgtatgg ccccatgtct gtgggtttgg acttctctct gccaggcatg 3841 gagcatgtct atgggatccc tgagcatgca gacaacctga ggctgaaggt cactgagggt 3901 ggggagccat atcgcctcta caatttggat gtgttccagt atgagctgta caacccaatg 3961 gccttgtatg ggtctgtgcc tgtgctcctg gcacacaacc ctcatcgcga cttgggcatc 4021 ttctggctca atgctgcaga gacctgggtt gatatatctt ccaacactgc cgggaagacc 4081 ctgtttggga agatgatgga ctacctgcag ggctctgggg agaccccaca gacagatgtt 4141 cgctggatgt cagagactgg catcattgac gtcttcctgc tgctggggcc ctccatctct 4201 gatgttttcc ggcaatatgc tagtctcaca ggaacccagg cgttgccccc actcttctcc 4261 ctcggctacc accagagccg ttggaactac cgggacgagg ctgatgtgct ggaagtggat 4321 cagggctttg atgatcacaa cctgccctgt gatgtcatct ggctagacat tgaacatgct 4381 gatggcaagc ggtatttcac ctgggacccc agtcgcttcc ctcagccccg caccatgctt 4441 gagcgcttgg cttctaagag gcggaagctg gtggccatcg tagaccccca catcaaggtg 4501 gactccggct accgagttca cgaggagctg cggaacctgg ggctgtatgt taaaacccgg 4561 gatggctctg actatgaggg ctggtgctgg ccaggctcag ctggttaccc tgacttcact 4621 aatcccacga tgagggcctg gtgggctaac atgttcagct atgacaatta tgagggctca 4681 gctcccaacc tctttgtctg gaatgacatg aacgaaccat ctgtgttcaa tggtcctgag 4741 gtcaccatgc tcaaggatgc ccagcattat gggggctggg agcaccggga tgtgcataac 4801 atctatggcc tttatgtgca catggcgact gctgatgggc tgagacagcg ctctgggggc 4861 atggaacgcc cctttgtcct ggccagggcc ttcttcgctg gctcccagcg ctttggagcc 4921 gtgtggacag gggacaacac tgccgagtgg gaccatttga agatctctat tcctatgtgt 4981 ctcagcttgg ggctggtggg actttccttc tgtggggcgg atgtgggtgg cttcttcaaa 5041 aacccagagc cagagctgct tgtgcgctgg taccagatgg gtgcttacca gccattcttc 5101 cgggcacatg cccacttgga cactgggcga cgagagccat ggctgttacc atctcagcac 5161 aatgatataa tccgagatgc cttgggccag cgatattctt tgctgccctt ctggtacacc 5221 ctcttatatc aggcccatcg ggaaggcatt cctgtcatga ggcccctgtg ggtgcagtac 5281 cctcaggatg tgactacctt caatatagat gatcagtact tgcttgggga tgcgttgctg 5341 gttcaccctg tatcagactc tggagcccat ggtgtccagg tctatctgcc tggccaaggg 5401 gaggtgtggt atgacattca aagctaccag aagcatcatg gtccccagac cctgtacctg 5461 cctgtaactc taagcagtat ccctgtgttc cagcgtggag ggacaatcgt gcctcgatgg 5521 atgcgagtgc ggcggtcttc agaatgtatg aaggatgacc ccatcactct ctttgttgca 5581 cttagccctc agggtacagc tcaaggagag ctctttctgg atgatgggta cacgttcaac 5641 tatcagactc gccaagagtt cctgctgcgt cgattctcat tctctggcaa cacccttgtc 5701 tccagctcag cagaccctga aggacacttt gagacaccaa tctggattga gcgggtggtg 5761 ataatagggg ctggaaagcc agcagctgtg gtactccaga caaaaggatc tccagaaagc 5821 cgcctgtcct tccagcatga ccctgagacc tctgtgttgg tcctgcgcaa gcctggcatc 5881 aatgtggcat ctgattggag tattcacctg cgaTAGGACC CAGCTTTCTT GTACAAAGTG 5941 GTTGATAGCT TGGATCCAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA 6001 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC 6061 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT 6121 CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG 6181 CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT 6241 TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT 6301 GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT 6361 CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT 6421 ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC 6481 GGCCTCTTCC GCGTCTTCGA GATCTGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT 6541 TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC 6601 CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG 6661 TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA 6721 CTCGAGTTAA GGGCGAATTG CCGATTAGGA TCTTCCTAGA GCATGGCTAC GTAGATAAGT 6781 AGCATGGCGG GTTAATCATT AACTACAAGG AACCCCTAGT GATGGAGTTG GCCACTCCCT 6841 CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA GGTCGCCCGA CGCCCGGGCT 6901 TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCCT TAATTAAGAG CTCATGGCGC 6961 GCCTAGGCCT TGACGGCCTT CCGCCAATTC GCCCTATAGT GAGTCGTATT ACGTCGCGCT 7021 CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC 7081 GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGAAA 7141 CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG AGCGCCCTGT AGCGGCCACT 7201 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 7261 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT 7321 TACAATTTAG //