LOCUS GANAB-pGEn2-DEST 8055 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 6683..7224 /note="WPRE" misc_recomb 6645..6667 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(7231..7445) /note="BGH pA" misc_feature complement(7533..7662) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" source 3891..6638 /dnas_title="GANAB-pGEn2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /sex="male" /tissue_type="Brain" /lab_host="Escherichia coli XL1-Blue" /plasmid="pACT2" gene 3891..6638 /dnas_title="KIAA0088" /gene="KIAA0088" CDS 3891..6638 /dnas_title="Glucosidase II" /gene="KIAA0088" /function="Glucose trimming of glycosylated proteins within the endoplasmic reticulum" /experiment="experimental evidence, no additional details recorded" /codon_start=1 /product="Glucosidase II" /protein_id="CAA04006.1" /db_xref="GI:2274968" /db_xref="GOA:Q14697" /db_xref="HGNC:4138" /db_xref="InterPro:IPR000322" /db_xref="UniProtKB/Swiss-Prot:Q14697" /translation="MAAVAAVAARRRRSWASLVLAFLGVCLGITLAVDRSNFKTCEES SFCKRQRSIRPGLSPYRALLDSLQLGPDSLTVHLIHEVTKVLLVLELQGLQKNMTRFR IDELEPRRPRYRVPDVLVADPPIARLSVSGRDENSVELTMAEGPYKIILTARPFRLDL LEDRSLLLSVNARGLLEFEHQRAPRVSQGSKDPAEGDGAQPEETPRDGDKPEETQGKA EKDEPGAWEETFKTHSDSKPYGPMSVGLDFSLPGMEHVYGIPEHADNLRLKVTEGGEP YRLYNLDVFQYELYNPMALYGSVPVLLAHNPHRDLGIFWLNAAETWVDISSNTAGKTL FGKMMDYLQGSGETPQTDVRWMSETGIIDVFLLLGPSISDVFRQYASLTGTQALPPLF SLGYHQSRWNYRDEADVLEVDQGFDDHNLPCDVIWLDIEHADGKRYFTWDPSRFPQPR TMLERLASKRRKLVAIVDPHIKVDSGYRVHEELRNLGLYVKTRDGSDYEGWCWPGSAG YPDFTNPTMRAWWANMFSYDNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAQHYGGW EHRDVHNIYGLYVHMATADGLRQRSGGMERPFVLARAFFAGSQRFGAVWTGDNTAEWD HLKISIPMCLSLGLVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTG RREPWLLPSQHNDIIRDALGQRYSLLPFWYTLLYQAHREGIPVMRPLWVQYPQDVTTF NIDDQYLLGDALLVHPVSDSGAHGVQVYLPGQGEVWYDIQSYQKHHGPQTLYLPVTLS SIPVFQRGGTIVPRWMRVRRSSECMKDDPITLFVALSPQGTAQGELFLDDGYTFNYQT RQEFLLRRFSFSGNTLVSSSADPEGHFETPIWIERVVIIGAGKPAAVVLQTKGSPESR LSFQHDPETSVLVLRKPGINVASDWSIHLR" sig_peptide 3891..3902 /dnas_title="KIAA0088" /gene="KIAA0088" mat_peptide 3903..6638 /dnas_title="Glucosidase II" /gene="KIAA0088" /product="Glucosidase II" /function="Glucose trimming of glycosylated proteins within the endoplasmic reticulum" misc_feature 3891..6638 /note="GANAB coding region" vector join(6653..8055,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..6652 /source="GANAB-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc attacccttg 3901 ctgtggatag aagcaacttt aagacctgtg aagagagttc tttctgcaag cgacagagaa 3961 gcatacggcc aggcctctct ccataccgag ccttgctgga ctctctacag cttggtcctg 4021 attccctcac ggtccatctg atccatgagg tcaccaaggt gttgctggtg ctagagcttc 4081 aggggcttca aaagaacatg actcggttca ggattgatga gctggagcct cggcgacccc 4141 gataccgtgt accagatgtt ttggtggctg atccaccaat agcccggctt tctgtctctg 4201 gtcgtgatga gaacagtgtg gagttaacca tggctgaggg accctacaag atcatcttga 4261 cagcacggcc attccgcctt gacctactag aggaccgaag tcttttgctt agtgtcaatg 4321 cccgaggact cttggagttt gagcatcaga gggcccctag ggtctcgcaa ggatcaaaag 4381 acccagctga gggcgatggg gcccagcctg aggaaacacc cagggatggc gacaagccag 4441 aggagactca ggggaaggca gagaaagatg agccaggagc ctgggaggag acattcaaaa 4501 ctcactctga cagcaagccg tatggcccca tgtctgtggg tttggacttc tctctgccag 4561 gcatggagca tgtctatggg atccctgagc atgcagacaa cctgaggctg aaggtcactg 4621 agggtgggga gccatatcgc ctctacaatt tggatgtgtt ccagtatgag ctgtacaacc 4681 caatggcctt gtatgggtct gtgcctgtgc tcctggcaca caaccctcat cgcgacttgg 4741 gcatcttctg gctcaatgct gcagagacct gggttgatat atcttccaac actgccggga 4801 agaccctgtt tgggaagatg atggactacc tgcagggctc tggggagacc ccacagacag 4861 atgttcgctg gatgtcagag actggcatca ttgacgtctt cctgctgctg gggccctcca 4921 tctctgatgt tttccggcaa tatgctagtc tcacaggaac ccaggcgttg cccccactct 4981 tctccctcgg ctaccaccag agccgttgga actaccggga cgaggctgat gtgctggaag 5041 tggatcaggg ctttgatgat cacaacctgc cctgtgatgt catctggcta gacattgaac 5101 atgctgatgg caagcggtat ttcacctggg accccagtcg cttccctcag ccccgcacca 5161 tgcttgagcg cttggcttct aagaggcgga agctggtggc catcgtagac ccccacatca 5221 aggtggactc cggctaccga gttcacgagg agctgcggaa cctggggctg tatgttaaaa 5281 cccgggatgg ctctgactat gagggctggt gctggccagg ctcagctggt taccctgact 5341 tcactaatcc cacgatgagg gcctggtggg ctaacatgtt cagctatgac aattatgagg 5401 gctcagctcc caacctcttt gtctggaatg acatgaacga accatctgtg ttcaatggtc 5461 ctgaggtcac catgctcaag gatgcccagc attatggggg ctgggagcac cgggatgtgc 5521 ataacatcta tggcctttat gtgcacatgg cgactgctga tgggctgaga cagcgctctg 5581 ggggcatgga acgccccttt gtcctggcca gggccttctt cgctggctcc cagcgctttg 5641 gagccgtgtg gacaggggac aacactgccg agtgggacca tttgaagatc tctattccta 5701 tgtgtctcag cttggggctg gtgggacttt ccttctgtgg ggcggatgtg ggtggcttct 5761 tcaaaaaccc agagccagag ctgcttgtgc gctggtacca gatgggtgct taccagccat 5821 tcttccgggc acatgcccac ttggacactg ggcgacgaga gccatggctg ttaccatctc 5881 agcacaatga tataatccga gatgccttgg gccagcgata ttctttgctg cccttctggt 5941 acaccctctt atatcaggcc catcgggaag gcattcctgt catgaggccc ctgtgggtgc 6001 agtaccctca ggatgtgact accttcaata tagatgatca gtacttgctt ggggatgcgt 6061 tgctggttca ccctgtatca gactctggag cccatggtgt ccaggtctat ctgcctggcc 6121 aaggggaggt gtggtatgac attcaaagct accagaagca tcatggtccc cagaccctgt 6181 acctgcctgt aactctaagc agtatccctg tgttccagcg tggagggaca atcgtgcctc 6241 gatggatgcg agtgcggcgg tcttcagaat gtatgaagga tgaccccatc actctctttg 6301 ttgcacttag ccctcagggt acagctcaag gagagctctt tctggatgat gggtacacgt 6361 tcaactatca gactcgccaa gagttcctgc tgcgtcgatt ctcattctct ggcaacaccc 6421 ttgtctccag ctcagcagac cctgaaggac actttgagac accaatctgg attgagcggg 6481 tggtgataat aggggctgga aagccagcag ctgtggtact ccagacaaaa ggatctccag 6541 aaagccgcct gtccttccag catgaccctg agacctctgt gttggtcctg cgcaagcctg 6601 gcatcaatgt ggcatctgat tggagtattc acctgcgaTA GGACCCAGCT TTCTTGTACA 6661 AAGTGGTTGA TAGCTTGGAT CCAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC 6721 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT 6781 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT 6841 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT 6901 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG 6961 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG 7021 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT 7081 GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT 7141 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC 7201 TCTGCGGCCT CTTCCGCGTC TTCGAGATCT GCCTCGACTG TGCCTTCTAG TTGCCAGCCA 7261 TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC 7321 CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG 7381 GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT 7441 GGGGACTCGA GTTAAGGGCG AATTGCCGAT TAGGATCTTC CTAGAGCATG GCTACGTAGA 7501 TAAGTAGCAT GGCGGGTTAA TCATTAACTA CAAGGAACCC CTAGTGATGG AGTTGGCCAC 7561 TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC 7621 GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCCTTAATT AAGAGCTCAT 7681 GGCGCGCCTA GGCCTTGACG GCCTTCCGCC AATTCGCCCT ATAGTGAGTC GTATTACGTC 7741 GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 7801 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 7861 CGAAACGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGAGCGC CCTGTAGCGG 7921 CCACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 7981 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA 8041 ACGCTTACAA TTTAG //