LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3437..3533) /label=attL2 /note="attL2 (c)" misc_signal complement(3548..3567) /note="T7 Promoter/priming site (c)" primer_bind 3575..3591 /note="M13 Reverse priming site" gene 3704..4513 /note="Kanamycin resistance gene" rep_origin 4634..5307 /note="pUC origin" vector join(3445..5310,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..3436 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:138256 IMAGE:8327519" /tissue_type="Colon, PCR rescued clones" /clone_lib="NIH_MGC_295" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: CCTTGGTG" gene 692..3436 /gene="GANC" /db_xref="GeneID:2595" /db_xref="HGNC:4139" /db_xref="IMGT/GENE-DB:4139" /db_xref="MIM:104180" CDS 692..3436 /dnas_title="glucosidase, alpha; neutral C" /gene="GANC" /codon_start=1 /product="glucosidase, alpha; neutral C" /protein_id="AAI12052.1" /db_xref="GI:85567132" /db_xref="GeneID:2595" /db_xref="HGNC:4139" /db_xref="IMGT/GENE-DB:4139" /db_xref="MIM:104180" /translation="MEAAVKEEISVEDEAVDKNIFRDCNKIAFYRRQKQWLSKKSTYQ ALLDSVTTDEDSTRFQIINEASKVPLLAEIYGIEGNIFRLKINEETPLKPRFEVPDVL TSKPSTVRLISCSGDTGSLILADGKGDLKCHITANPFKVDLVSEEEVVISINSLGQLY FEHLQILHKQRAAKENEEETSVDTSQENQEDLGLWEEKFGKFVDIKANGPSSIGLDFS LHGFEHLYGIPQHAESHQLKNTGDGDAYRLYNLDVYGYQIYDKMGIYGSVPYLLAHKL GRTIGIFWLNASETLVEINTEPAVEYTLTQMGPVAAKQKVRSRTHVHWMSESGIIDVF LLTGPTPSDVFKQYSHLTGTQAMPPLFSLGYHQCRWNYEDEQDVKAVDAGFDEHDIPY DAMWLDIEHTEGKRYFTWDKNRFPNPKRMQELLRSKKRKLVVISDPHIKIDPDYSVYV KAKDQGFFVKNQEGEDFEGVCWPGLSSYLDFTNPKVREWYSSLFAFPVYQGSTDILFL WNDMNEPSVFRGPEQTMQKNAIHHGNWEHRELHNIYGFYHQMATAEGLIKRSKGKERP FVLTRSFFAGSQKYGAVWTGDNTAEWSNLKISIPMLLTLSITGISFCGADIGGFIGNP ETELLVRWYQAGAYQPFFRGHATMNTKRREPWLFGEEHTRLIREAIRERYGLLPYWYS LFYHAHVASQPVMRPLWVEFPDELKTFDMEDEYMLGSALLVHPVTEPKATTVDVFLPG SNEVWYDYKTFAHWEGGCTVKIPVALDTIPVFQRGGSVIPIKTTVGKSTGWMTESSYG LRVALSTKGSSVGELYLDDGHSFQYLHQKQFLHRKFSFCSSVLINSSADQRGHYPSKC VVEKILVLGFRKEPSSVTTHSSDGKDQPVAFTYCAKTSILSLEKLSLNIATDWEVRII " misc_difference 722..722 /gene="GANC" /note="'G' in cDNA is 'C' in the human genome; amino acid difference: 'V' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_feature 692..3436 /note="GANC coding region" misc_difference 3225..3225 /gene="GANC" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'S' in cDNA, 'F' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg catggaagca gcagtgaaag aggaaataag 721 tgttgaagat gaagctgtag ataaaaacat tttcagagac tgtaacaaga tcgcatttta 781 caggcgtcag aaacagtggc tttccaagaa gtccacctat caggcattat tggattcagt 841 cacaacagat gaagacagca ccaggttcca aatcatcaat gaagcaagta aggttcctct 901 cctggctgaa atttatggta tagaaggaaa cattttcagg cttaaaatta atgaagagac 961 tcctctaaaa cccagatttg aagttccgga tgtcctcaca agcaagccaa gcactgtaag 1021 gctgatttca tgctctgggg acacaggcag tctgatattg gcagatggaa aaggagacct 1081 gaagtgccat atcacagcaa acccattcaa ggtagacttg gtgtctgaag aagaggttgt 1141 gattagcata aattccctgg gccaattata ctttgagcat ctacagattc ttcacaaaca 1201 aagagctgct aaagaaaatg aggaggagac atcagtggac acctctcagg aaaatcaaga 1261 agatctgggc ctgtgggaag agaaatttgg aaaatttgtg gatatcaaag ctaatggccc 1321 ttcttctatt ggtttggatt tctccttgca tggatttgag catctttatg ggatcccaca 1381 acatgcagaa tcacaccaac ttaaaaatac tggtgatgga gatgcttacc gtctttataa 1441 cctggatgtc tatggatacc aaatatatga taaaatgggc atttatggtt cagtacctta 1501 tctcctggcc cacaaactgg gcagaactat aggtattttc tggctgaatg cctcggaaac 1561 actggtggag atcaatacag agcctgcagt agagtacaca ctgacccaga tgggcccagt 1621 tgctgctaaa caaaaggtca gatctcgcac tcatgtgcac tggatgtcag agagtggcat 1681 cattgatgtt tttctgctga caggacctac accttctgat gtcttcaaac agtactcaca 1741 ccttacaggc acacaagcca tgccccctct tttctctttg ggataccacc agtgccgctg 1801 gaactatgaa gatgagcagg atgtaaaagc agtggatgca gggtttgatg agcatgacat 1861 tccttatgat gccatgtggc tggacataga gcacactgag ggcaagaggt acttcacctg 1921 ggacaaaaac agattcccaa accccaagag gatgcaagag ctgctcagga gcaaaaagcg 1981 taagcttgtg gtcatcagtg atccccacat caagattgat cctgactact cagtatatgt 2041 gaaggccaaa gatcagggct tctttgtgaa gaatcaggaa ggggaagact ttgaaggggt 2101 gtgttggcca ggtctctcct cttacctgga tttcaccaat cccaaggtca gagagtggta 2161 ttcaagtctt tttgctttcc ctgtttatca gggatctacg gacatcctct tcctttggaa 2221 tgacatgaat gagccttctg tctttagagg gccagagcaa accatgcaga agaatgccat 2281 tcatcatggc aattgggagc acagagagct ccacaacatc tacggttttt atcatcaaat 2341 ggctactgca gaaggactga taaaacgatc taaagggaag gagagaccct ttgttcttac 2401 acgttctttc tttgctggat cacaaaagta tggtgccgtg tggacaggcg acaacacagc 2461 agaatggagc aacttgaaaa tttctatccc aatgttactc actctcagca ttactgggat 2521 ctctttttgc ggagctgaca taggcgggtt cattgggaat ccagagacag agctgctagt 2581 gcgttggtac caggctggag cctaccagcc cttcttccgt ggccatgcca ccatgaacac 2641 caagcgacga gagccctggc tctttgggga ggaacacacc cgactcatcc gagaagccat 2701 cagagagcgc tatggcctcc tgccatattg gtattctctg ttctaccatg cacacgtggc 2761 ttcccaacct gtcatgaggc ctctgtgggt agagttccct gatgaactaa agacttttga 2821 tatggaagat gaatacatgc tggggagtgc attattggtt catccagtca cagaaccaaa 2881 agccaccaca gttgatgtgt ttcttccagg atcaaatgag gtctggtatg actataagac 2941 atttgctcat tgggaaggag ggtgtactgt aaagatccca gtagccttgg acactattcc 3001 agtgtttcag cgaggtggaa gtgtgatacc aataaagaca actgtaggaa aatccacagg 3061 ctggatgact gaatcctcct atggactccg ggttgctcta agcactaagg gttcttcagt 3121 gggtgagtta tatcttgatg atggccattc attccaatac ctccaccaga agcaattttt 3181 gcacaggaag ttttcattct gttccagtgt tctgatcaat agttctgctg accagagggg 3241 tcattatccc agcaagtgtg tggtggagaa gatcttggtc ttaggcttca ggaaggagcc 3301 atcttctgtg actacccact catctgatgg taaagatcag cctgtggctt ttacgtattg 3361 tgccaaaaca tccatcctga gcctggagaa gctctcactc aacattgcca ctgactggga 3421 ggtccgcatc atatgaCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT 3481 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC 3541 AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG 3601 GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAATAATAT CATCATGAAC 3661 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG 3721 GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG 3781 GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA 3841 TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA 3901 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT 3961 CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA 4021 GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT 4081 GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG 4141 TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA 4201 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT 4261 CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA 4321 GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA 4381 TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT 4441 TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA 4501 TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC 4561 TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA 4621 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 4681 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 4741 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 4801 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 4861 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 4921 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 4981 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 5041 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 5101 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 5161 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 5221 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 5281 GGCCTTTTGC TGGCCTTTTG CTCACATGTT //