LOCUS GANC-pGEn1-DEST 7321 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 5949..6490 /note="WPRE" misc_recomb 5911..5933 /label=attB2 /note="attB2 site" misc_feature complement(6497..6711) /note="BGH pA" misc_feature complement(6799..6928) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..5910 /dnas_title="GANC-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:138256 IMAGE:8327519" /tissue_type="Colon, PCR rescued clones" /clone_lib="NIH_MGC_295" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: CCTTGGTG" gene 3166..5910 /dnas_title="GANC" /gene="GANC" /db_xref="GeneID:2595" /db_xref="HGNC:4139" /db_xref="IMGT/GENE-DB:4139" /db_xref="MIM:104180" CDS 3166..5910 /dnas_title="glucosidase, alpha; neutral C" /gene="GANC" /codon_start=1 /product="glucosidase, alpha; neutral C" /protein_id="AAI12052.1" /db_xref="GI:85567132" /db_xref="GeneID:2595" /db_xref="HGNC:4139" /db_xref="IMGT/GENE-DB:4139" /db_xref="MIM:104180" /translation="MEAAVKEEISVEDEAVDKNIFRDCNKIAFYRRQKQWLSKKSTYQ ALLDSVTTDEDSTRFQIINEASKVPLLAEIYGIEGNIFRLKINEETPLKPRFEVPDVL TSKPSTVRLISCSGDTGSLILADGKGDLKCHITANPFKVDLVSEEEVVISINSLGQLY FEHLQILHKQRAAKENEEETSVDTSQENQEDLGLWEEKFGKFVDIKANGPSSIGLDFS LHGFEHLYGIPQHAESHQLKNTGDGDAYRLYNLDVYGYQIYDKMGIYGSVPYLLAHKL GRTIGIFWLNASETLVEINTEPAVEYTLTQMGPVAAKQKVRSRTHVHWMSESGIIDVF LLTGPTPSDVFKQYSHLTGTQAMPPLFSLGYHQCRWNYEDEQDVKAVDAGFDEHDIPY DAMWLDIEHTEGKRYFTWDKNRFPNPKRMQELLRSKKRKLVVISDPHIKIDPDYSVYV KAKDQGFFVKNQEGEDFEGVCWPGLSSYLDFTNPKVREWYSSLFAFPVYQGSTDILFL WNDMNEPSVFRGPEQTMQKNAIHHGNWEHRELHNIYGFYHQMATAEGLIKRSKGKERP FVLTRSFFAGSQKYGAVWTGDNTAEWSNLKISIPMLLTLSITGISFCGADIGGFIGNP ETELLVRWYQAGAYQPFFRGHATMNTKRREPWLFGEEHTRLIREAIRERYGLLPYWYS LFYHAHVASQPVMRPLWVEFPDELKTFDMEDEYMLGSALLVHPVTEPKATTVDVFLPG SNEVWYDYKTFAHWEGGCTVKIPVALDTIPVFQRGGSVIPIKTTVGKSTGWMTESSYG LRVALSTKGSSVGELYLDDGHSFQYLHQKQFLHRKFSFCSSVLINSSADQRGHYPSKC VVEKILVLGFRKEPSSVTTHSSDGKDQPVAFTYCAKTSILSLEKLSLNIATDWEVRII " misc_difference 3196..3196 /dnas_title="GANC" /gene="GANC" /note="'G' in cDNA is 'C' in the human genome; amino acid difference: 'V' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_feature 3166..5910 /note="GANC coding region" misc_difference 5699..5699 /dnas_title="GANC" /gene="GANC" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'S' in cDNA, 'F' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." vector join(5919..7321,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..5918 /source="GANC-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcatgga agcagcagtg 3181 aaagaggaaa taagtgttga agatgaagct gtagataaaa acattttcag agactgtaac 3241 aagatcgcat tttacaggcg tcagaaacag tggctttcca agaagtccac ctatcaggca 3301 ttattggatt cagtcacaac agatgaagac agcaccaggt tccaaatcat caatgaagca 3361 agtaaggttc ctctcctggc tgaaatttat ggtatagaag gaaacatttt caggcttaaa 3421 attaatgaag agactcctct aaaacccaga tttgaagttc cggatgtcct cacaagcaag 3481 ccaagcactg taaggctgat ttcatgctct ggggacacag gcagtctgat attggcagat 3541 ggaaaaggag acctgaagtg ccatatcaca gcaaacccat tcaaggtaga cttggtgtct 3601 gaagaagagg ttgtgattag cataaattcc ctgggccaat tatactttga gcatctacag 3661 attcttcaca aacaaagagc tgctaaagaa aatgaggagg agacatcagt ggacacctct 3721 caggaaaatc aagaagatct gggcctgtgg gaagagaaat ttggaaaatt tgtggatatc 3781 aaagctaatg gcccttcttc tattggtttg gatttctcct tgcatggatt tgagcatctt 3841 tatgggatcc cacaacatgc agaatcacac caacttaaaa atactggtga tggagatgct 3901 taccgtcttt ataacctgga tgtctatgga taccaaatat atgataaaat gggcatttat 3961 ggttcagtac cttatctcct ggcccacaaa ctgggcagaa ctataggtat tttctggctg 4021 aatgcctcgg aaacactggt ggagatcaat acagagcctg cagtagagta cacactgacc 4081 cagatgggcc cagttgctgc taaacaaaag gtcagatctc gcactcatgt gcactggatg 4141 tcagagagtg gcatcattga tgtttttctg ctgacaggac ctacaccttc tgatgtcttc 4201 aaacagtact cacaccttac aggcacacaa gccatgcccc ctcttttctc tttgggatac 4261 caccagtgcc gctggaacta tgaagatgag caggatgtaa aagcagtgga tgcagggttt 4321 gatgagcatg acattcctta tgatgccatg tggctggaca tagagcacac tgagggcaag 4381 aggtacttca cctgggacaa aaacagattc ccaaacccca agaggatgca agagctgctc 4441 aggagcaaaa agcgtaagct tgtggtcatc agtgatcccc acatcaagat tgatcctgac 4501 tactcagtat atgtgaaggc caaagatcag ggcttctttg tgaagaatca ggaaggggaa 4561 gactttgaag gggtgtgttg gccaggtctc tcctcttacc tggatttcac caatcccaag 4621 gtcagagagt ggtattcaag tctttttgct ttccctgttt atcagggatc tacggacatc 4681 ctcttccttt ggaatgacat gaatgagcct tctgtcttta gagggccaga gcaaaccatg 4741 cagaagaatg ccattcatca tggcaattgg gagcacagag agctccacaa catctacggt 4801 ttttatcatc aaatggctac tgcagaagga ctgataaaac gatctaaagg gaaggagaga 4861 ccctttgttc ttacacgttc tttctttgct ggatcacaaa agtatggtgc cgtgtggaca 4921 ggcgacaaca cagcagaatg gagcaacttg aaaatttcta tcccaatgtt actcactctc 4981 agcattactg ggatctcttt ttgcggagct gacataggcg ggttcattgg gaatccagag 5041 acagagctgc tagtgcgttg gtaccaggct ggagcctacc agcccttctt ccgtggccat 5101 gccaccatga acaccaagcg acgagagccc tggctctttg gggaggaaca cacccgactc 5161 atccgagaag ccatcagaga gcgctatggc ctcctgccat attggtattc tctgttctac 5221 catgcacacg tggcttccca acctgtcatg aggcctctgt gggtagagtt ccctgatgaa 5281 ctaaagactt ttgatatgga agatgaatac atgctgggga gtgcattatt ggttcatcca 5341 gtcacagaac caaaagccac cacagttgat gtgtttcttc caggatcaaa tgaggtctgg 5401 tatgactata agacatttgc tcattgggaa ggagggtgta ctgtaaagat cccagtagcc 5461 ttggacacta ttccagtgtt tcagcgaggt ggaagtgtga taccaataaa gacaactgta 5521 ggaaaatcca caggctggat gactgaatcc tcctatggac tccgggttgc tctaagcact 5581 aagggttctt cagtgggtga gttatatctt gatgatggcc attcattcca atacctccac 5641 cagaagcaat ttttgcacag gaagttttca ttctgttcca gtgttctgat caatagttct 5701 gctgaccaga ggggtcatta tcccagcaag tgtgtggtgg agaagatctt ggtcttaggc 5761 ttcaggaagg agccatcttc tgtgactacc cactcatctg atggtaaaga tcagcctgtg 5821 gcttttacgt attgtgccaa aacatccatc ctgagcctgg agaagctctc actcaacatt 5881 gccactgact gggaggtccg catcatatga CCAGCTTTCT TGTACAAAGT GGTTGATAGC 5941 TTGGATCCAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT 6001 ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG 6061 CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG 6121 AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA 6181 CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC 6241 CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG 6301 CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT 6361 GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT 6421 CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC 6481 CGCGTCTTCG AGATCTGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC 6541 CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA 6601 TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG 6661 GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ACTCGAGTTA 6721 AGGGCGAATT GCCGATTAGG ATCTTCCTAG AGCATGGCTA CGTAGATAAG TAGCATGGCG 6781 GGTTAATCAT TAACTACAAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG 6841 CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG 6901 CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC TTAATTAAGA GCTCATGGCG CGCCTAGGCC 6961 TTGACGGCCT TCCGCCAATT CGCCCTATAG TGAGTCGTAT TACGTCGCGC TCACTGGCCG 7021 TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG 7081 CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAA ACGCCCTTCC 7141 CAACAGTTGC GCAGCCTGAA TGGCGAATGG GAGCGCCCTG TAGCGGCCAC TCAACCCTAT 7201 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 7261 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA 7321 G //