LOCUS GANC-pGEn2-DEST 8046 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 6674..7215 /note="WPRE" misc_recomb 6636..6658 /label=attB2 /note="attB2 site" misc_feature complement(7222..7436) /note="BGH pA" misc_feature complement(7524..7653) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" source 3891..6635 /dnas_title="GANC-pGEn2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:138256 IMAGE:8327519" /tissue_type="Colon, PCR rescued clones" /clone_lib="NIH_MGC_295" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: CCTTGGTG" gene 3891..6635 /dnas_title="GANC" /gene="GANC" /db_xref="GeneID:2595" /db_xref="HGNC:4139" /db_xref="IMGT/GENE-DB:4139" /db_xref="MIM:104180" CDS 3891..6635 /dnas_title="glucosidase, alpha; neutral C" /gene="GANC" /codon_start=1 /product="glucosidase, alpha; neutral C" /protein_id="AAI12052.1" /db_xref="GI:85567132" /db_xref="GeneID:2595" /db_xref="HGNC:4139" /db_xref="IMGT/GENE-DB:4139" /db_xref="MIM:104180" /translation="MEAAVKEEISVEDEAVDKNIFRDCNKIAFYRRQKQWLSKKSTYQ ALLDSVTTDEDSTRFQIINEASKVPLLAEIYGIEGNIFRLKINEETPLKPRFEVPDVL TSKPSTVRLISCSGDTGSLILADGKGDLKCHITANPFKVDLVSEEEVVISINSLGQLY FEHLQILHKQRAAKENEEETSVDTSQENQEDLGLWEEKFGKFVDIKANGPSSIGLDFS LHGFEHLYGIPQHAESHQLKNTGDGDAYRLYNLDVYGYQIYDKMGIYGSVPYLLAHKL GRTIGIFWLNASETLVEINTEPAVEYTLTQMGPVAAKQKVRSRTHVHWMSESGIIDVF LLTGPTPSDVFKQYSHLTGTQAMPPLFSLGYHQCRWNYEDEQDVKAVDAGFDEHDIPY DAMWLDIEHTEGKRYFTWDKNRFPNPKRMQELLRSKKRKLVVISDPHIKIDPDYSVYV KAKDQGFFVKNQEGEDFEGVCWPGLSSYLDFTNPKVREWYSSLFAFPVYQGSTDILFL WNDMNEPSVFRGPEQTMQKNAIHHGNWEHRELHNIYGFYHQMATAEGLIKRSKGKERP FVLTRSFFAGSQKYGAVWTGDNTAEWSNLKISIPMLLTLSITGISFCGADIGGFIGNP ETELLVRWYQAGAYQPFFRGHATMNTKRREPWLFGEEHTRLIREAIRERYGLLPYWYS LFYHAHVASQPVMRPLWVEFPDELKTFDMEDEYMLGSALLVHPVTEPKATTVDVFLPG SNEVWYDYKTFAHWEGGCTVKIPVALDTIPVFQRGGSVIPIKTTVGKSTGWMTESSYG LRVALSTKGSSVGELYLDDGHSFQYLHQKQFLHRKFSFCSSVLINSSADQRGHYPSKC VVEKILVLGFRKEPSSVTTHSSDGKDQPVAFTYCAKTSILSLEKLSLNIATDWEVRII " misc_difference 3921..3921 /dnas_title="GANC" /gene="GANC" /note="'G' in cDNA is 'C' in the human genome; amino acid difference: 'V' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_feature 3891..6635 /note="GANC coding region" misc_difference 6424..6424 /dnas_title="GANC" /gene="GANC" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'S' in cDNA, 'F' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." vector join(6644..8046,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..6643 /source="GANC-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc atggaagcag 3901 cagtgaaaga ggaaataagt gttgaagatg aagctgtaga taaaaacatt ttcagagact 3961 gtaacaagat cgcattttac aggcgtcaga aacagtggct ttccaagaag tccacctatc 4021 aggcattatt ggattcagtc acaacagatg aagacagcac caggttccaa atcatcaatg 4081 aagcaagtaa ggttcctctc ctggctgaaa tttatggtat agaaggaaac attttcaggc 4141 ttaaaattaa tgaagagact cctctaaaac ccagatttga agttccggat gtcctcacaa 4201 gcaagccaag cactgtaagg ctgatttcat gctctgggga cacaggcagt ctgatattgg 4261 cagatggaaa aggagacctg aagtgccata tcacagcaaa cccattcaag gtagacttgg 4321 tgtctgaaga agaggttgtg attagcataa attccctggg ccaattatac tttgagcatc 4381 tacagattct tcacaaacaa agagctgcta aagaaaatga ggaggagaca tcagtggaca 4441 cctctcagga aaatcaagaa gatctgggcc tgtgggaaga gaaatttgga aaatttgtgg 4501 atatcaaagc taatggccct tcttctattg gtttggattt ctccttgcat ggatttgagc 4561 atctttatgg gatcccacaa catgcagaat cacaccaact taaaaatact ggtgatggag 4621 atgcttaccg tctttataac ctggatgtct atggatacca aatatatgat aaaatgggca 4681 tttatggttc agtaccttat ctcctggccc acaaactggg cagaactata ggtattttct 4741 ggctgaatgc ctcggaaaca ctggtggaga tcaatacaga gcctgcagta gagtacacac 4801 tgacccagat gggcccagtt gctgctaaac aaaaggtcag atctcgcact catgtgcact 4861 ggatgtcaga gagtggcatc attgatgttt ttctgctgac aggacctaca ccttctgatg 4921 tcttcaaaca gtactcacac cttacaggca cacaagccat gccccctctt ttctctttgg 4981 gataccacca gtgccgctgg aactatgaag atgagcagga tgtaaaagca gtggatgcag 5041 ggtttgatga gcatgacatt ccttatgatg ccatgtggct ggacatagag cacactgagg 5101 gcaagaggta cttcacctgg gacaaaaaca gattcccaaa ccccaagagg atgcaagagc 5161 tgctcaggag caaaaagcgt aagcttgtgg tcatcagtga tccccacatc aagattgatc 5221 ctgactactc agtatatgtg aaggccaaag atcagggctt ctttgtgaag aatcaggaag 5281 gggaagactt tgaaggggtg tgttggccag gtctctcctc ttacctggat ttcaccaatc 5341 ccaaggtcag agagtggtat tcaagtcttt ttgctttccc tgtttatcag ggatctacgg 5401 acatcctctt cctttggaat gacatgaatg agccttctgt ctttagaggg ccagagcaaa 5461 ccatgcagaa gaatgccatt catcatggca attgggagca cagagagctc cacaacatct 5521 acggttttta tcatcaaatg gctactgcag aaggactgat aaaacgatct aaagggaagg 5581 agagaccctt tgttcttaca cgttctttct ttgctggatc acaaaagtat ggtgccgtgt 5641 ggacaggcga caacacagca gaatggagca acttgaaaat ttctatccca atgttactca 5701 ctctcagcat tactgggatc tctttttgcg gagctgacat aggcgggttc attgggaatc 5761 cagagacaga gctgctagtg cgttggtacc aggctggagc ctaccagccc ttcttccgtg 5821 gccatgccac catgaacacc aagcgacgag agccctggct ctttggggag gaacacaccc 5881 gactcatccg agaagccatc agagagcgct atggcctcct gccatattgg tattctctgt 5941 tctaccatgc acacgtggct tcccaacctg tcatgaggcc tctgtgggta gagttccctg 6001 atgaactaaa gacttttgat atggaagatg aatacatgct ggggagtgca ttattggttc 6061 atccagtcac agaaccaaaa gccaccacag ttgatgtgtt tcttccagga tcaaatgagg 6121 tctggtatga ctataagaca tttgctcatt gggaaggagg gtgtactgta aagatcccag 6181 tagccttgga cactattcca gtgtttcagc gaggtggaag tgtgatacca ataaagacaa 6241 ctgtaggaaa atccacaggc tggatgactg aatcctccta tggactccgg gttgctctaa 6301 gcactaaggg ttcttcagtg ggtgagttat atcttgatga tggccattca ttccaatacc 6361 tccaccagaa gcaatttttg cacaggaagt tttcattctg ttccagtgtt ctgatcaata 6421 gttctgctga ccagaggggt cattatccca gcaagtgtgt ggtggagaag atcttggtct 6481 taggcttcag gaaggagcca tcttctgtga ctacccactc atctgatggt aaagatcagc 6541 ctgtggcttt tacgtattgt gccaaaacat ccatcctgag cctggagaag ctctcactca 6601 acattgccac tgactgggag gtccgcatca tatgaCCAGC TTTCTTGTAC AAAGTGGTTG 6661 ATAGCTTGGA TCCAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT 6721 TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC 6781 TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT 6841 TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA 6901 CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC 6961 TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC 7021 AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC TGACGTCCTT 7081 TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT 7141 CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC 7201 TCTTCCGCGT CTTCGAGATC TGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT 7261 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA 7321 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG 7381 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGACTCG 7441 AGTTAAGGGC GAATTGCCGA TTAGGATCTT CCTAGAGCAT GGCTACGTAG ATAAGTAGCA 7501 TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT 7561 GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC 7621 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCCTTAAT TAAGAGCTCA TGGCGCGCCT 7681 AGGCCTTGAC GGCCTTCCGC CAATTCGCCC TATAGTGAGT CGTATTACGT CGCGCTCACT 7741 GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT 7801 TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGAAACGCC 7861 CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGAGCG CCCTGTAGCG GCCACTCAAC 7921 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 7981 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA 8041 ATTTAG //