LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(6161..6257) /label=attL2 /note="attL2 (c)" misc_signal complement(6272..6291) /note="T7 Promoter/priming site (c)" primer_bind 6299..6315 /note="M13 Reverse priming site" gene 6428..7237 /note="Kanamycin resistance gene" rep_origin 7358..8031 /note="pUC origin" vector join(6169..8034,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..6160 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:134955 IMAGE:40073922" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_283" /note="Vector: pCR-Blunt II-TOPO with reversed insert; Clone identification sequence tag: NA" gene 692..6160 /gene="MGAM" /gene_synonym=MG /gene_synonym=MGA /db_xref="GeneID:8972" /db_xref="HGNC:7043" /db_xref="MIM:154360" CDS 692..6160 /dnas_title="maltase-glucoamylase (alpha-glucosidase)" /gene="MGAM" /gene_synonym=MG /gene_synonym=MGA /codon_start=1 /product="maltase-glucoamylase (alpha-glucosidase)" /protein_id="AAI20873.1" /db_xref="GI:111308926" /db_xref="GeneID:8972" /db_xref="HGNC:7043" /db_xref="MIM:154360" /translation="MARKKLKKFTTLEIVLSVLLLVLFIISIVLIVLLAKESLKSTAP DPGTTGTPDPGTTGTPDPGTTGTTHARTTGPPDPGTTGTTPVSAECPVVNELERINCI PDQPPTKATCDQRGCCWNPQGAVSVPWCYYSKNHSYHVEGNLVNTNAGFTARLKNLPS SPVFGSNVDNVLLTAEYQTSNRFHFKLTDQTNNRFEVPHEHVQSFSGNAAASLTYQVE ISRQPFSIKVTRRSNNRVLFDSSIGPLLFADQFLQLSTRLPSTNVYGLGEHVHQQYRH DMNWKTWPIFNRDTTPNGNGTNLYGAQTFFLCLEDASGLSFGVFLMNSNAMEVVLQPA PAITYRTIGGILDFYVFLGNTPEQVVQEYLELIGRPALPSYWALGFHLSRYEYGTLDN MREVVERNRAAQLPYDVQHADIDYMDERRDFTYDSVDFKGFPEFVNELHNNGQKLVII VDPAISNNSSSSKPYGPYDRGSDMKIWVNSSDGVTPLIGEVWPGQTVFPDYTNPNCAV WWTKEFELFHNQVEFDGIWIDMNEVSNFVDGSVSGCSTNNLNNPPFTPRILDGYLFCK TLCMDAVQHWGKQYDIHNLYGYSMAVATAEAAKTVFPNKRSFILTRSTFAGSGKFAAH WLGDNTATWDDLRWSIPGVLEFNLFGIPMVGPDICGFALDTPEELCRRWMQLGAFYPF SRNHNGQGYKDQDPASFGADSLLLNSSRHYLNIRYTLLPYLYTLFFRAHSRGDTVARP LLHEFYEDNSTWDVHQQFLWGPGLLITPVLDEGAEKVMAYVPDAVWYDYETGSQVRWR KQKVEMELPGDKIGLHLRGGYIFPTQQPNTTTLASRKNPLGLIIALDENKEAKGEPFW DDGETKDTVANKVYLLCEFSVTQNRLEVNISQSTYKDPNNLAFNEIKILGTEEPSNVT VKHNGVPSQTSPTVTYDSNLKVAIITDIDLLLGEAYTVEWSIKIRDEEKIDCYPDENG ASAENCTARGCIWEASNSSGVPFCYFVNDLYSVSDVQYNSHGATADISLKSSVYANAF PSTPVNPLRLDVTYHKNEMLQFKIYDPNKNRYEVPVPLNIPSMPSSTPEGQLYDVLIK KNPFGIEIRRKSTGTIIWDSQLLGFTFSDMFIRISTRLPSKYLYGFGETEHRSYRRDL EWHTWGMFSRDQPPGYKKNSYGVHPYYMGLEEDGSAHGVLLLNSNAMDVTFQPLPALT YRTTGGVLDFYVFLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASL YDEMVAAQIPYDVQYSDIDYMERQLDFTLSPKFAGFPALINRMKADGMRVILILDPAI SGNETQPYPAFTRGVEDDVFIKYPNDGDIVWGKVWPDFPDVVVNGSLDWDSQVELYRA YVAFPDFFRNSTAKWWKREIEELYNNPQNPERSLKFDGMWIDMNEPSSFVNGAVSPGC RDASLNHPPYMPHLESRDRGLSSKTLCMESQQILPDGSLVQHYNVHNLYGWSQTRPTY EAVQEVTGQRGVVITRSTFPSSGRWAGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYT GADICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTRRQDPVSWDVAFVNISRTVLQ TRYTLLPYLYTLMHKAHTEGVTVVRPLLHEFVSDQVTWDIDSQFLLGPAFLVSPVLER NARNVTAYFPRARWYDYYTGVDINARGEWKTLPAPLDHINLHVRGGYILPWQEPALNT HLSRQKFMGFKIALDDEGTAGGWLFWDDGQSIDTYGKGLYYLASFSASQNTMQSHIIF NNYITGTNPLKLGYIEIWGVGSVPVTSVSISVSGMVITPSFNNDPTTQVLSIDVTDRN ISLHNFTSLTWISTL" misc_feature 692..6160 /note="MGAM coding region" misc_difference 3147..3147 /gene="MGAM" /gene_synonym=MG /gene_synonym=MGA /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'P' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 3158..3158 /gene="MGAM" /gene_synonym=MG /gene_synonym=MGA /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'D' in cDNA, 'N' in the human genome." misc_difference 3901..3901 /gene="MGAM" /gene_synonym=MG /gene_synonym=MGA /note="'C' in cDNA is 'T' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 4555..4555 /gene="MGAM" /gene_synonym=MG /gene_synonym=MGA /note="'C' in cDNA is 'T' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 5506..5506 /gene="MGAM" /gene_synonym=MG /gene_synonym=MGA /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caaagagtca ctgaaatcaa cagccccaga 721 tcctgggaca actggtaccc cagatcctgg gacaactggt accccagatc ctggaacaac 781 tggtaccaca catgctagga caacgggtcc cccagatcct ggaacaactg gtaccactcc 841 tgtttctgct gaatgtccag tggtaaatga attggaacga attaattgca tccctgacca 901 gccgccaaca aaggccacat gtgaccaacg tggctgttgc tggaatcccc agggagctgt 961 aagtgttccc tggtgctact attccaagaa tcatagctac catgtagagg gcaaccttgt 1021 caacacaaat gcaggattca cagcccggtt gaaaaatctg ccttcttcac cagtgtttgg 1081 aagcaatgtt gacaatgttc ttctcacagc agaatatcag acatctaatc gtttccactt 1141 taagttgact gaccaaacca ataacaggtt tgaagtgccc cacgaacacg tgcagtcctt 1201 cagtggaaat gctgctgctt ctttgaccta ccaagttgaa atctccagac agccatttag 1261 catcaaagtg accagaagaa gcaacaatcg tgttttgttt gactcgagca ttgggcccct 1321 actgtttgct gaccagttct tgcagctctc cactcgactg cctagcacta acgtgtatgg 1381 cctgggagag catgtgcacc agcagtatcg gcatgatatg aattggaaga cctggcccat 1441 atttaacaga gacacaactc ccaatggaaa cggaactaat ttgtatggtg cgcagacatt 1501 cttcttgtgc cttgaagatg ctagtggatt gtcctttggg gtgtttctga tgaacagcaa 1561 tgccatggag gttgtccttc agcctgcgcc agccatcact taccgcacca ttgggggcat 1621 tctcgacttc tatgtgttct tgggaaacac tccagagcaa gttgttcaag aatatctaga 1681 gctcattggg cggccagccc ttccctccta ctgggcgctt ggatttcacc tcagtcgtta 1741 cgaatatgga accttagaca acatgaggga agtcgtggag agaaatcgcg cagcacagct 1801 cccttatgat gttcagcatg ctgatattga ttatatggat gagagaaggg acttcactta 1861 tgattcagtg gattttaaag gcttccctga atttgtcaac gagttacaca ataatggaca 1921 gaagcttgtc atcattgtgg atccagccat ctccaacaac tcttcctcaa gtaaacccta 1981 tggcccatat gacaggggtt cagatatgaa gatatgggtg aatagttcag atggagtgac 2041 tccactcatt ggggaggtct ggcctggaca aactgtgttt cctgattata ccaatcccaa 2101 ctgtgctgtt tggtggacaa aggaatttga gctttttcac aatcaagtag agtttgatgg 2161 aatctggatt gatatgaatg aagtctccaa ctttgttgat ggttcggtct caggatgttc 2221 cacaaacaac ctaaataatc ccccattcac tcccagaatc ctggatgggt acctgttctg 2281 caagactctc tgtatggatg cagtgcagca ctggggcaag cagtatgaca ttcacaatct 2341 gtatggctac tccatggcgg tcgccacagc agaagctgcc aagactgtgt tccctaataa 2401 gagaagcttc attctgaccc gttctacctt tgcgggctct ggcaagtttg cagcacattg 2461 gttaggagac aacactgcca cctgggatga cctgagatgg tccatccctg gcgtgcttga 2521 gttcaacctt tttggcatcc caatggtggg tcctgacata tgtggctttg ctttggacac 2581 ccctgaggag ctctgtaggc ggtggatgca gttgggtgca ttttatccgt tttctagaaa 2641 tcacaatggc caaggctaca aggaccagga tcctgcctcc tttggagctg actccctgct 2701 gttgaattcc tccaggcact accttaacat ccgctatact ctattgccct acctatacac 2761 cctcttcttc cgtgctcaca gccgagggga cacggtggcc aggccccttt tgcatgagtt 2821 ctacgaggac aacagcactt gggatgtgca ccaacagttc ttatgggggc ccggcctcct 2881 catcactcca gttctggatg aaggtgcaga gaaagtgatg gcatatgtgc ctgatgctgt 2941 ctggtatgac tacgagactg ggagccaagt gagatggagg aagcaaaaag tcgagatgga 3001 acttcctgga gacaaaattg gacttcacct tcgaggaggc tacatcttcc ccacacagca 3061 gccaaataca accactctgg ccagtcgaaa gaaccctctt ggtcttatca ttgccctaga 3121 tgagaacaaa gaagcaaaag gagaaccttt ctgggatgat ggggaaacga aggatactgt 3181 ggccaataaa gtgtatcttt tatgtgagtt ttctgtcact caaaaccgct tggaggtgaa 3241 tatttcacaa tcaacctaca aggaccccaa taatttagca tttaatgaga ttaaaattct 3301 tgggacggag gaacctagca atgttacagt gaaacacaat ggtgtcccaa gtcagacttc 3361 tcctacagtc acttatgatt ctaacctgaa ggttgccatt atcacagata ttgatcttct 3421 cctgggagaa gcatacacag tggaatggag cataaagata agggatgaag aaaaaataga 3481 ctgttaccct gatgagaatg gtgcttctgc cgaaaactgc actgcccgtg gctgtatctg 3541 ggaggcatcc aattcttctg gagtcccttt ttgctatttt gtcaacgacc tatactctgt 3601 cagtgatgtt cagtataatt cccatggggc cacagctgac atctccttaa agtcttccgt 3661 ttatgccaat gccttcccct ccacacccgt gaaccccctt cgcctggatg tcacttacca 3721 taagaatgaa atgctgcagt tcaagattta tgatcccaac aagaatcggt atgaagttcc 3781 agtccctctg aacataccca gcatgccatc cagcacccct gagggtcaac tctatgatgt 3841 gctcattaag aagaatccat ttgggattga aattcgccgg aagagtacag gcactataat 3901 ctgggactct cagctccttg gctttacctt cagtgacatg tttatccgca tctccacccg 3961 ccttccctcc aagtacctct atggctttgg ggaaactgag cacaggtcct ataggagaga 4021 cttggagtgg cacacttggg ggatgttctc ccgagaccag cccccagggt acaagaagaa 4081 ttcctatggt gtccacccct actacatggg gctggaggag gacggcagtg cccatggagt 4141 gctcctgctg aacagcaatg ccatggatgt gacgttccag cccctgcctg ccttgacata 4201 ccgcaccaca gggggagttc tggactttta tgtgttcttg gggccgactc cagagcttgt 4261 cacccagcag tacactgagt tgattggccg gcctgtgatg gtaccttact ggtctttggg 4321 gttccagctg tgtcgctatg gctaccagaa tgactctgag atcgccagct tgtatgatga 4381 gatggtggct gcccagatcc cttatgatgt gcagtactca gacatcgact acatggagcg 4441 gcagctggac ttcaccctca gccccaagtt tgctgggttt ccagctctga tcaatcgcat 4501 gaaggctgat gggatgcggg tcatcctcat tctggatcca gccatttctg gcaacgagac 4561 acagccttat cctgccttca ctcggggcgt ggaggatgac gtcttcatca aatacccaaa 4621 tgatggagac attgtctggg gaaaggtctg gcctgatttt cctgatgttg ttgtgaatgg 4681 gtctctagac tgggacagcc aagtggagct atatcgagct tatgtggcct tcccagactt 4741 tttccgtaat tcaactgcca agtggtggaa gagggaaata gaagaactat acaacaatcc 4801 acagaatcca gagaggagct tgaagtttga tggcatgtgg attgatatga atgaaccatc 4861 aagcttcgtg aatggggcag tttctccagg ctgcagggac gcctctctga accaccctcc 4921 ctacatgcca catttggagt ccagggacag gggcctgagc agcaagaccc tttgtatgga 4981 gagtcagcag atcctcccag acggctccct ggtgcagcac tacaacgtgc acaacctgta 5041 tgggtggtcc cagaccagac ccacatacga agccgtgcag gaggtgacgg gacagcgagg 5101 ggtcgtcatc acccgctcca catttccctc ttctggccgc tgggcaggac attggctggg 5161 agacaacacg gccgcatggg atcagctgaa gaagtctatc attggcatga tggagttcag 5221 cctcttcggc atatcctata cgggagcaga tatctgtggg ttctttcaag atgctgaata 5281 tgagatgtgt gttcgctgga tgcagctggg ggccttttac cccttctcaa gaaaccacaa 5341 caccattggg accaggagac aagaccctgt gtcctgggat gttgcttttg tgaatatttc 5401 cagaactgtc ctgcagacca gatacaccct gttgccatat ctgtatacct tgatgcataa 5461 ggcccacacg gagggcgtca ctgttgtgcg gcctctgctc catgaatttg tgtcagacca 5521 ggtgacatgg gacatagaca gtcagttcct gctgggccca gccttcctgg tcagccctgt 5581 cctggagcgt aatgccagaa atgtcactgc atatttccct agagcccgct ggtatgatta 5641 ctacacgggt gtggatatta atgcaagagg agagtggaag accttgccag cccctcttga 5701 ccacattaat cttcatgtcc gtgggggcta catcctgccc tggcaagagc ctgcactgaa 5761 cacccactta agccgccaga aattcatggg cttcaaaatt gccttggatg atgaaggaac 5821 tgctgggggc tggctcttct gggatgatgg gcaaagcatt gatacctatg ggaaaggact 5881 ctattacttg gccagctttt ctgccagcca gaatacgatg caaagccata taattttcaa 5941 caattacatc actggtacaa atcctttgaa actgggctac attgaaatct ggggagtggg 6001 cagtgtcccc gttaccagtg tcagcatctc tgtgagtggc atggtcataa caccctcctt 6061 caacaatgac cccacgacac aggtattaag catcgatgtg actgacagaa acatcagcct 6121 acataatttt acttcattga cgtggataag cactctgtga CCAGCTTTCT TGTACAAAGT 6181 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 6241 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 6301 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA 6361 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG 6421 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA 6481 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT 6541 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG 6601 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 6661 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC 6721 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT 6781 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC 6841 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT 6901 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 6961 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC 7021 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT 7081 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC 7141 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA 7201 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 7261 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT 7321 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 7381 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 7441 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 7501 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 7561 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 7621 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 7681 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 7741 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 7801 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 7861 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 7921 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 7981 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT //