LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1940..2036) /label=attL2 /note="attL2 (c)" misc_signal complement(2051..2070) /note="T7 Promoter/priming site (c)" primer_bind 2078..2094 /note="M13 Reverse priming site" gene 2207..3016 /note="Kanamycin resistance gene" rep_origin 3137..3810 /note="pUC origin" PCR_frame 669..1940 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..1960) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1957) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1948..3813,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1918..1938 /note="TEV site" source 673..1917 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1553 IMAGE:3506824" /tissue_type="Kidney, renal cell adenocarcinoma" /clone_lib="NIH_MGC_14" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..1917 /gene="NEU1" /gene_synonym=SIAL1 /db_xref="GeneID:4758" /db_xref="HGNC:7758" /db_xref="MIM:608272" CDS 673..1917 /dnas_title="sialidase 1 (lysosomal sialidase)" /gene="NEU1" /gene_synonym=SIAL1 /codon_start=1 /product="sialidase 1 (lysosomal sialidase)" /protein_id="AAH00722.1" /db_xref="GI:12653863" /db_xref="GeneID:4758" /db_xref="HGNC:7758" /db_xref="MIM:608272" /translation="MTGERPSTALPDRRWGPRILGFWGGCRVWVFAAIFLLLSLAASW SKAENDFGLVQPLVTMEQLLWVSGRQIGSVDTFRIPLITATPRGTLLAFAEARKMSSS DEGAKFIALRRSMDQGSTWSPTAFIVNDGDVPDGLNLGAVVSDVETGVVFLFYSLCAH KAGCQVASTMLVWSKDDGVSWSTPRNLSLDIGTEVFAPGPGSGIQKQREPRKGRLIVC GHGTLERDGVFCLLSDDHGASWRYGSGVSGIPYGQPKQENDFNPDECQPYELPDGSVV INARNQNNYHCHCRIVLRSYDACDTLRPRDVTFDPELVDPVVAAGAVVTSSGIVFFSN PAHPEFRVNLTLRWSFSNGTSWRKETVQLWPGPSGYSSLATLEGSMDGEEQAPQLYVL YEKGRNHYTESISVAKISVYGTL" misc_feature 673..1917 /note="NEU1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgactgg ggagcgaccc agcacggcgc tcccggacag acgctggggg 721 ccgcggattc tgggcttctg gggaggctgt agggtttggg tgtttgccgc gatcttcctg 781 ctgctgtctc tggcagcctc ctggtccaag gctgagaacg acttcggtct ggtgcagccg 841 ctggtgacca tggagcaact gctgtgggtg agcgggagac agatcggctc agtggacacc 901 ttccgcatcc cgctcatcac agccactccg cggggcactc ttctcgcctt tgctgaggcg 961 aggaaaatgt cctcatccga tgagggggcc aagttcatcg ccctgcggag gtccatggac 1021 cagggcagca catggtctcc tacagcgttc attgtcaatg atggggatgt ccccgatggg 1081 ctgaaccttg gggcagtagt gagcgatgtt gagacaggag tagtatttct tttctactcc 1141 ctttgtgctc acaaggccgg ctgccaggtg gcctctacca tgttggtatg gagcaaggat 1201 gatggtgttt cctggagcac accccggaat ctctccctgg atattggcac tgaagtgttt 1261 gcccctggac cgggctctgg tattcagaaa cagcgggagc cacggaaggg ccgcctcatc 1321 gtgtgtggcc atgggacgct ggagcgggac ggagtcttct gtctcctcag cgatgatcat 1381 ggtgcctcct ggcgctacgg aagtggggtc agcggcatcc cctacggtca gcccaagcag 1441 gaaaatgatt tcaatcctga tgaatgccag ccctatgagc tcccagatgg ctcagtcgtc 1501 atcaatgccc gaaaccagaa caactaccac tgccactgcc gaattgtcct ccgcagctat 1561 gatgcctgtg atacactaag gccccgtgat gtgaccttcg accctgagct cgtggaccct 1621 gtggtagctg caggagctgt agtcaccagc tccggcattg tcttcttctc caacccagca 1681 catccagagt tccgagtgaa cctgaccctg cgatggagct tcagcaatgg tacctcatgg 1741 cggaaagaga cagtccagct atggccaggc cccagtggct attcatccct ggcaaccctg 1801 gagggcagca tggatggaga ggagcaggcc ccccagctct acgtcctgta tgagaaaggc 1861 cggaaccact acacagagag catctccgtg gccaaaatca gtgtctatgg gacactcgaa 1921 aacttgtact ttcaaggctC CAGCTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG 1981 CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA 2041 TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT 2101 CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAATAA TATCATCATG 2161 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA 2221 ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA 2281 ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC 2341 CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA 2401 TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT 2461 TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT 2521 CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT 2581 CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT 2641 TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA 2701 TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC 2761 ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA 2821 CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA 2881 GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT 2941 TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT 3001 CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT 3061 GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT 3121 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 3181 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 3241 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 3301 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 3361 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 3421 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 3481 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 3541 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 3601 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 3661 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 3721 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 3781 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT //