LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1799..1895)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1910..1929)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1937..1953
                     /note="M13 Reverse priming site"
     gene            2066..2875
                     /note="Kanamycin resistance gene"
     rep_origin      2996..3669
                     /note="pUC origin"
     vector          join(1807..3672,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1798
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:1553 IMAGE:3506824"
                     /tissue_type="Kidney, renal cell adenocarcinoma"
                     /clone_lib="NIH_MGC_14"
                     /lab_host="DH10B-R"
                     /note="Vector: pOTB7"
     gene            692..1798
                     /gene="NEU1"
                     /gene_synonym=SIAL1
                     /db_xref="GeneID:4758"
                     /db_xref="HGNC:7758"
                     /db_xref="MIM:608272"
     CDS             692..1798
                     /dnas_title="sialidase 1 (lysosomal sialidase)"
                     /gene="NEU1"
                     /gene_synonym=SIAL1
                     /codon_start=1
                     /product="sialidase 1 (lysosomal sialidase)"
                     /protein_id="AAH00722.1"
                     /db_xref="GI:12653863"
                     /db_xref="GeneID:4758"
                     /db_xref="HGNC:7758"
                     /db_xref="MIM:608272"
                     /translation="MTGERPSTALPDRRWGPRILGFWGGCRVWVFAAIFLLLSLAASW
                     SKAENDFGLVQPLVTMEQLLWVSGRQIGSVDTFRIPLITATPRGTLLAFAEARKMSSS
                     DEGAKFIALRRSMDQGSTWSPTAFIVNDGDVPDGLNLGAVVSDVETGVVFLFYSLCAH
                     KAGCQVASTMLVWSKDDGVSWSTPRNLSLDIGTEVFAPGPGSGIQKQREPRKGRLIVC
                     GHGTLERDGVFCLLSDDHGASWRYGSGVSGIPYGQPKQENDFNPDECQPYELPDGSVV
                     INARNQNNYHCHCRIVLRSYDACDTLRPRDVTFDPELVDPVVAAGAVVTSSGIVFFSN
                     PAHPEFRVNLTLRWSFSNGTSWRKETVQLWPGPSGYSSLATLEGSMDGEEQAPQLYVL
                     YEKGRNHYTESISVAKISVYGTL"
     misc_feature    692..1798
                     /note="NEU1 coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cgagaacgac ttcggtctgg tgcagccgct
        721 ggtgaccatg gagcaactgc tgtgggtgag cgggagacag atcggctcag tggacacctt
        781 ccgcatcccg ctcatcacag ccactccgcg gggcactctt ctcgcctttg ctgaggcgag
        841 gaaaatgtcc tcatccgatg agggggccaa gttcatcgcc ctgcggaggt ccatggacca
        901 gggcagcaca tggtctccta cagcgttcat tgtcaatgat ggggatgtcc ccgatgggct
        961 gaaccttggg gcagtagtga gcgatgttga gacaggagta gtatttcttt tctactccct
       1021 ttgtgctcac aaggccggct gccaggtggc ctctaccatg ttggtatgga gcaaggatga
       1081 tggtgtttcc tggagcacac cccggaatct ctccctggat attggcactg aagtgtttgc
       1141 ccctggaccg ggctctggta ttcagaaaca gcgggagcca cggaagggcc gcctcatcgt
       1201 gtgtggccat gggacgctgg agcgggacgg agtcttctgt ctcctcagcg atgatcatgg
       1261 tgcctcctgg cgctacggaa gtggggtcag cggcatcccc tacggtcagc ccaagcagga
       1321 aaatgatttc aatcctgatg aatgccagcc ctatgagctc ccagatggct cagtcgtcat
       1381 caatgcccga aaccagaaca actaccactg ccactgccga attgtcctcc gcagctatga
       1441 tgcctgtgat acactaaggc cccgtgatgt gaccttcgac cctgagctcg tggaccctgt
       1501 ggtagctgca ggagctgtag tcaccagctc cggcattgtc ttcttctcca acccagcaca
       1561 tccagagttc cgagtgaacc tgaccctgcg atggagcttc agcaatggta cctcatggcg
       1621 gaaagagaca gtccagctat ggccaggccc cagtggctat tcatccctgg caaccctgga
       1681 gggcagcatg gatggagagg agcaggcccc ccagctctac gtcctgtatg agaaaggccg
       1741 gaaccactac acagagagca tctccgtggc caaaatcagt gtctatggga cactctgaCC
       1801 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA
       1861 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG
       1921 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT
       1981 CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA ACAATAAAAC TGTCTGCTTA
       2041 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG
       2101 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG
       2161 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT
       2221 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG
       2281 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC
       2341 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC
       2401 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT
       2461 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC
       2521 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC
       2581 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT
       2641 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG
       2701 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA
       2761 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA
       2821 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA
       2881 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG
       2941 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT
       3001 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
       3061 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
       3121 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA
       3181 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
       3241 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
       3301 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
       3361 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA
       3421 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
       3481 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
       3541 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
       3601 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
       3661 TGCTCACATG TT
//