LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1835..1931) /label=attL2 /note="attL2 (c)" misc_signal complement(1946..1965) /note="T7 Promoter/priming site (c)" primer_bind 1973..1989 /note="M13 Reverse priming site" gene 2102..2911 /note="Kanamycin resistance gene" rep_origin 3032..3705 /note="pUC origin" vector join(1843..3708,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1834 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:95436 IMAGE:7217011" /tissue_type="Synthetic constructs" /clone_lib="NIH_MGC_243" /lab_host="TOP10" /note="Vector: pCR Blunt II TOPO" gene 692..1834 /gene="NEU2" /gene_synonym=SIAL2 /db_xref="GeneID:4759" /db_xref="HGNC:7759" /db_xref="MIM:605528" CDS 692..1834 /dnas_title="sialidase 2 (cytosolic sialidase)" /gene="NEU2" /gene_synonym=SIAL2 /codon_start=1 /product="sialidase 2 (cytosolic sialidase)" /protein_id="AAH69151.1" /db_xref="GI:46575656" /db_xref="GeneID:4759" /db_xref="HGNC:7759" /db_xref="MIM:605528" /translation="MASLPVLQKESVFQSGAHAYRIPALLYLPGQQSLLAFAEQRASK KDEHAELIVLRRGDYDAPTHQVQWQAQEVVAQARLDGHRSMNPCPLYDAQTGTLFLFF IAIPGQVTEQQQLQTRANVTRLCQVTSTDHGRTWSSPRDLTDAAIGPAYREWSTFAVG PGHCLQLHDRARSLVVPAYAYRKLHPIQRPIPSAFCFLSHDHGRTWARGHFVAQDTLE CQVAEVETGEQRVVTLNARSHLRARVQAQSTNDGLDFQESQLVKKLVEPPPQGCQGSV ISFPSPRSGPGSPAQWLLYTHPTHSWQRADLGAYLNPRPPAPEAWSEPVLLAKGSCAY SDLQSMGTGPDGSPLFGCLYEANDYEEIVFLMFTLKQAFPAEYLPQ" misc_feature 692..1834 /gene="NEU2" /gene_synonym=SIAL2 /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" misc_feature 692..1834 /note="NEU2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg catggcgtcc cttcctgtcc tgcagaagga 721 gagcgtgttc cagtcgggag cccatgccta cagaatccct gccctgctct acctgcctgg 781 gcagcagtcc ctgctggcct tcgcggaaca gcgggcaagc aagaaggatg agcacgcaga 841 gctgattgtc ctgcgcagag gagactacga cgcacccacc caccaggttc agtggcaagc 901 tcaggaggtg gtggcccagg cccggctgga tggccaccgg tccatgaacc catgcccctt 961 gtatgacgcg cagacgggga ccctcttcct cttcttcatt gccatccctg ggcaagtcac 1021 ggagcaacag cagctgcaga ccagggccaa tgtgacgcgg ctgtgccaag tcaccagcac 1081 tgaccacggg aggacctgga gctcccccag agacctcact gatgcggcca tcggcccagc 1141 ctaccgggag tggtccacct ttgcagtggg cccggggcat tgtttgcagc ttcacgacag 1201 ggcccggagc ctggtggtgc ccgcctacgc ctaccggaaa cttcacccca tccaaaggcc 1261 gatcccctct gccttctgct tcctcagcca tgaccatggg cgcacgtggg cgcgagggca 1321 ctttgtggcc caggacaccc tggagtgcca ggtggccgaa gtcgagactg gggagcagag 1381 ggtggtgacc ctcaacgcga gaagccacct ccgagccagg gtccaggccc agagcaccaa 1441 tgacgggctt gatttccagg agtctcagct ggtgaagaag ctggtggagc cgccgcccca 1501 gggctgccag gggagcgtca tcagcttccc cagcccccgc tcggggcctg gctccccagc 1561 ccagtggctg ctctacactc accccacaca ctcctggcag agggccgacc tgggtgccta 1621 cctcaacccg cgacctccag cccctgaggc ctggtcagag ccggtactgc tggccaaggg 1681 cagctgtgcc tactcagacc tccagagcat gggcaccggc cctgatgggt cccccttgtt 1741 tgggtgtctg tacgaagcca atgattacga ggagattgtc tttctcatgt tcaccctgaa 1801 gcaagccttc ccagctgagt acctgcctca gtgaCCAGCT TTCTTGTACA AAGTTGGCAT 1861 TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA 1921 AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC 1981 TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA 2041 AATAATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT 2101 TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA 2161 TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG 2221 CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC 2281 CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC 2341 GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC 2401 CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA 2461 TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA 2521 CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA 2581 TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT 2641 GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA 2701 TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT 2761 CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC 2821 ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA 2881 GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG 2941 GCAGAGCATT ACGCTGACTT GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT 3001 GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 3061 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 3121 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 3181 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 3241 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 3301 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 3361 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 3421 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG 3481 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 3541 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 3601 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 3661 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTT //