LOCUS NEU4-pGEc1-DEST 5983 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5461..5590) /note="3'-ITR" misc_feature complement(5159..5373) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4611..5152 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_recomb 4516..4538 /label=attB2 /note="attB2 site" misc_feature 4548..4571 /note="8xHis Tag" misc_feature 4575..4598 /note="StrepTag" PCR_frame 3002..4516 /note="Reading Frame" /set="Set 1" source 3006..4493 /dnas_title="NEU4-pGEc1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:18222 IMAGE:4156395" /tissue_type="Brain, anaplastic oligodendroglioma with 1p/19q loss" /clone_lib="NCI_CGAP_Brn67" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4493 /dnas_title="NEU4" /gene="NEU4" /gene_synonym="MGC18222" /db_xref="GeneID:129807" /db_xref="HGNC:21328" /db_xref="MIM:608527" CDS 3006..4493 /dnas_title="sialidase 4" /gene="NEU4" /gene_synonym="MGC18222" /codon_start=1 /product="sialidase 4" /protein_id="AAH12899.2" /db_xref="GI:49208223" /db_xref="GeneID:129807" /db_xref="HGNC:21328" /db_xref="MIM:608527" /translation="MMSSAAFPRWLSMGVPRTPSRTVLFERERTGLTYRVPSLLPVPP GPTLLAFVEQRLSPDDSHAHRLVLRRGTLAGGSVRWGALHVLGTAALAEHRSMNPCPV HDAGTGTVFLFFIAVLGHTPEAVQIATGRNAARLCCVASRDAGLSWGSARDLTEEAIG GAVQDWATFAVGPGHGVQLPSGRLLVPAYTYRVDRRECFGKICRTSPHSFAFYSDDHG RTWRCGGLVPNLRSGECQLAAVDGGQAGSFLYCNARSPLGSRVQALSTDEGTSFLPAE RVASLPETAWGCQGSIVGFPAPAPNRPRDDSWSVGPGSPLQPPLLGPGVHEPPEEAAV DPRGGQVPGGPFSRLQPRGDGPRQPGPRPGVSGDVGSWTLALPMPFAAPPQSPTWLLY SHPVGRRARLHMGIRLSQSPLDPRSWTEPWVIYEGPSGYSDLASIGPAPEGGLVFACL YESGARTSYDEISFCTFSLREVLENVPASPKPPNLGDKPRGCCWPS" misc_feature 3006..4493 /note="Neu4 coding region" misc_feature 4494..4514 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4523) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4523) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4524..5983,1..2991) /source="pGEc1-DEST" /type="Destination Vector" clone 2992..4523 /source="Neu4 C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgat gagctctgca gccttcccaa ggtggctgag catgggggtc cctcgtaccc 3061 cttcacggac agtgctcttc gagcgggaga ggacgggcct gacctaccgc gtgccctcgc 3121 tgctccccgt gccccccggg cccaccctgc tggcctttgt ggagcagcgg ctcagccctg 3181 acgactccca cgcccaccgc ctggtgctga ggaggggcac gctggccggg ggctccgtgc 3241 ggtggggtgc cctgcacgtg ctggggacag cagccctggc ggagcaccgg tccatgaacc 3301 cctgccctgt gcacgatgct ggcacgggca ccgtcttcct cttcttcatc gcggtgctgg 3361 gccacacgcc tgaggccgtg cagatcgcca cgggaaggaa cgccgcgcgc ctctgctgtg 3421 tggccagccg tgacgccggc ctctcgtggg gcagcgcccg ggacctcacc gaggaggcca 3481 tcggtggtgc cgtgcaggac tgggccacat tcgctgtggg tcccggccac ggcgtgcagc 3541 tgccctcagg ccgcctgctg gtacccgcct acacctaccg cgtggaccgc cgagagtgtt 3601 ttggcaagat ctgccggacc agccctcact ccttcgcctt ctacagcgat gaccacggcc 3661 gcacctggcg ctgtggaggc ctcgtgccca acctgcgctc aggcgagtgc cagctggcgg 3721 cggtggacgg tgggcaggcc ggcagcttcc tctactgcaa tgcccggagc ccactgggca 3781 gccgtgtgca ggcgctcagc actgacgagg gcacctcctt cctgcccgca gagcgcgtgg 3841 cttccctgcc cgagactgcc tggggctgcc agggcagcat cgtgggcttc ccagcccccg 3901 cccccaacag gccacgggat gacagttggt cagtgggccc cgggagtccc ctccagcctc 3961 cactcctcgg tcctggagtc cacgaacccc cagaggaggc tgctgtagac ccccgtggag 4021 gccaggtgcc tggtgggccc ttcagccgtc tgcagcctcg gggggatggc cccaggcagc 4081 ctggccccag gcctggggtc agtggggatg tggggtcctg gaccctggca ctccccatgc 4141 cctttgctgc cccgccccag agccccacgt ggctgctgta ctcccaccca gtggggcgca 4201 gggctcggct acacatgggt atccgcctga gccagtcccc gctggacccg cgcagctgga 4261 cagagccctg ggtgatctac gagggcccca gcggctactc cgacctggcg tccatcgggc 4321 cggctcctga ggggggcctg gtttttgcct gcctgtacga gagcggggcc aggacctcct 4381 atgatgagat ttccttttgt acattctccc tgcgtgaggt cctggagaac gtgcccgcca 4441 gccccaagcc gcccaacctt ggggacaagc ctcgggggtg ctgctggccc tccgaaaact 4501 tgtactttca aggctCCAGC TTTCTTGTAC AAAGTGGTGA TGGCCGCCAT CATCATCATC 4561 ATCATCATCA TGCGTGGAGC CACCCGCAGT TCGAAAAATA ATAAGGATCC AATCAACCTC 4621 TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC 4681 TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA 4741 TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG 4801 TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA 4861 TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG 4921 CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG 4981 ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG 5041 CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG 5101 ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGAGATCTGC 5161 CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT 5221 GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA 5281 TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA 5341 GGATTGGGAA GACAATAGCA GGCATGCTGG GGACTCGAGT TAAGGGCGAA TTGCCGATTA 5401 GGATCTTCCT AGAGCATGGC TACGTAGATA AGTAGCATGG CGGGTTAATC ATTAACTACA 5461 AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG 5521 CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC 5581 GAGCGCGCAG CCTTAATTAA GAGCTCATGG CGCGCCTAGG CCTTGACGGC CTTCCGCCAA 5641 TTCGCCCTAT AGTGAGTCGT ATTACGTCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG 5701 ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA 5761 GCTGGCGTAA TAGCGAAGAG GCCCGCACCG AAACGCCCTT CCCAACAGTT GCGCAGCCTG 5821 AATGGCGAAT GGGAGCGCCC TGTAGCGGCC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA 5881 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 5941 ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TAG //