LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2183..2279) /label=attL2 /note="attL2 (c)" misc_signal complement(2294..2313) /note="T7 Promoter/priming site (c)" primer_bind 2321..2337 /note="M13 Reverse priming site" gene 2450..3259 /note="Kanamycin resistance gene" rep_origin 3380..4053 /note="pUC origin" PCR_frame 669..2183 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2203) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2200) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2191..4056,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2161..2181 /note="TEV site" source 673..2160 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:18222 IMAGE:4156395" /tissue_type="Brain, anaplastic oligodendroglioma with 1p/19q loss" /clone_lib="NCI_CGAP_Brn67" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..2160 /gene="NEU4" /gene_synonym=MGC18222 /db_xref="GeneID:129807" /db_xref="HGNC:21328" /db_xref="MIM:608527" CDS 673..2160 /dnas_title="sialidase 4" /gene="NEU4" /gene_synonym=MGC18222 /codon_start=1 /product="sialidase 4" /protein_id="AAH12899.2" /db_xref="GI:49208223" /db_xref="GeneID:129807" /db_xref="HGNC:21328" /db_xref="MIM:608527" /translation="MMSSAAFPRWLSMGVPRTPSRTVLFERERTGLTYRVPSLLPVPP GPTLLAFVEQRLSPDDSHAHRLVLRRGTLAGGSVRWGALHVLGTAALAEHRSMNPCPV HDAGTGTVFLFFIAVLGHTPEAVQIATGRNAARLCCVASRDAGLSWGSARDLTEEAIG GAVQDWATFAVGPGHGVQLPSGRLLVPAYTYRVDRRECFGKICRTSPHSFAFYSDDHG RTWRCGGLVPNLRSGECQLAAVDGGQAGSFLYCNARSPLGSRVQALSTDEGTSFLPAE RVASLPETAWGCQGSIVGFPAPAPNRPRDDSWSVGPGSPLQPPLLGPGVHEPPEEAAV DPRGGQVPGGPFSRLQPRGDGPRQPGPRPGVSGDVGSWTLALPMPFAAPPQSPTWLLY SHPVGRRARLHMGIRLSQSPLDPRSWTEPWVIYEGPSGYSDLASIGPAPEGGLVFACL YESGARTSYDEISFCTFSLREVLENVPASPKPPNLGDKPRGCCWPS" misc_feature 673..2160 /note="Neu4 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgatgag ctctgcagcc ttcccaaggt ggctgagcat gggggtccct 721 cgtacccctt cacggacagt gctcttcgag cgggagagga cgggcctgac ctaccgcgtg 781 ccctcgctgc tccccgtgcc ccccgggccc accctgctgg cctttgtgga gcagcggctc 841 agccctgacg actcccacgc ccaccgcctg gtgctgagga ggggcacgct ggccgggggc 901 tccgtgcggt ggggtgccct gcacgtgctg gggacagcag ccctggcgga gcaccggtcc 961 atgaacccct gccctgtgca cgatgctggc acgggcaccg tcttcctctt cttcatcgcg 1021 gtgctgggcc acacgcctga ggccgtgcag atcgccacgg gaaggaacgc cgcgcgcctc 1081 tgctgtgtgg ccagccgtga cgccggcctc tcgtggggca gcgcccggga cctcaccgag 1141 gaggccatcg gtggtgccgt gcaggactgg gccacattcg ctgtgggtcc cggccacggc 1201 gtgcagctgc cctcaggccg cctgctggta cccgcctaca cctaccgcgt ggaccgccga 1261 gagtgttttg gcaagatctg ccggaccagc cctcactcct tcgccttcta cagcgatgac 1321 cacggccgca cctggcgctg tggaggcctc gtgcccaacc tgcgctcagg cgagtgccag 1381 ctggcggcgg tggacggtgg gcaggccggc agcttcctct actgcaatgc ccggagccca 1441 ctgggcagcc gtgtgcaggc gctcagcact gacgagggca cctccttcct gcccgcagag 1501 cgcgtggctt ccctgcccga gactgcctgg ggctgccagg gcagcatcgt gggcttccca 1561 gcccccgccc ccaacaggcc acgggatgac agttggtcag tgggccccgg gagtcccctc 1621 cagcctccac tcctcggtcc tggagtccac gaacccccag aggaggctgc tgtagacccc 1681 cgtggaggcc aggtgcctgg tgggcccttc agccgtctgc agcctcgggg ggatggcccc 1741 aggcagcctg gccccaggcc tggggtcagt ggggatgtgg ggtcctggac cctggcactc 1801 cccatgccct ttgctgcccc gccccagagc cccacgtggc tgctgtactc ccacccagtg 1861 gggcgcaggg ctcggctaca catgggtatc cgcctgagcc agtccccgct ggacccgcgc 1921 agctggacag agccctgggt gatctacgag ggccccagcg gctactccga cctggcgtcc 1981 atcgggccgg ctcctgaggg gggcctggtt tttgcctgcc tgtacgagag cggggccagg 2041 acctcctatg atgagatttc cttttgtaca ttctccctgc gtgaggtcct ggagaacgtg 2101 cccgccagcc ccaagccgcc caaccttggg gacaagcctc gggggtgctg ctggccctcc 2161 gaaaacttgt actttcaagg ctCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 2221 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG 2281 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA 2341 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC 2401 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT 2461 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA 2521 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA 2581 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC 2641 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA 2701 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC 2761 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT 2821 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT 2881 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT 2941 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT 3001 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT 3061 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA 3121 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG 3181 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT 3241 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC 3301 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC 3361 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 3421 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 3481 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 3541 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 3601 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 3661 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 3721 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 3781 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 3841 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 3901 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 3961 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 4021 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT //