LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2183..2279) /label=attL2 /note="attL2 (c)" misc_signal complement(2294..2313) /note="T7 Promoter/priming site (c)" primer_bind 2321..2337 /note="M13 Reverse priming site" gene 2450..3259 /note="Kanamycin resistance gene" rep_origin 3380..4053 /note="pUC origin" vector join(2191..4056,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2182 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:18222 IMAGE:4156395" /tissue_type="Brain, anaplastic oligodendroglioma with 1p/19q loss" /clone_lib="NCI_CGAP_Brn67" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..2182 /gene="NEU4" /gene_synonym=MGC18222 /db_xref="GeneID:129807" /db_xref="HGNC:21328" /db_xref="MIM:608527" CDS 692..2182 /dnas_title="sialidase 4" /gene="NEU4" /gene_synonym=MGC18222 /codon_start=1 /product="sialidase 4" /protein_id="AAH12899.2" /db_xref="GI:49208223" /db_xref="GeneID:129807" /db_xref="HGNC:21328" /db_xref="MIM:608527" /translation="MMSSAAFPRWLSMGVPRTPSRTVLFERERTGLTYRVPSLLPVPP GPTLLAFVEQRLSPDDSHAHRLVLRRGTLAGGSVRWGALHVLGTAALAEHRSMNPCPV HDAGTGTVFLFFIAVLGHTPEAVQIATGRNAARLCCVASRDAGLSWGSARDLTEEAIG GAVQDWATFAVGPGHGVQLPSGRLLVPAYTYRVDRRECFGKICRTSPHSFAFYSDDHG RTWRCGGLVPNLRSGECQLAAVDGGQAGSFLYCNARSPLGSRVQALSTDEGTSFLPAE RVASLPETAWGCQGSIVGFPAPAPNRPRDDSWSVGPGSPLQPPLLGPGVHEPPEEAAV DPRGGQVPGGPFSRLQPRGDGPRQPGPRPGVSGDVGSWTLALPMPFAAPPQSPTWLLY SHPVGRRARLHMGIRLSQSPLDPRSWTEPWVIYEGPSGYSDLASIGPAPEGGLVFACL YESGARTSYDEISFCTFSLREVLENVPASPKPPNLGDKPRGCCWPS" misc_feature 692..2182 /note="NEU4 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg catgatgagc tctgcagcct tcccaaggtg 721 gctgagcatg ggggtccctc gtaccccttc acggacagtg ctcttcgagc gggagaggac 781 gggcctgacc taccgcgtgc cctcgctgct ccccgtgccc cccgggccca ccctgctggc 841 ctttgtggag cagcggctca gccctgacga ctcccacgcc caccgcctgg tgctgaggag 901 gggcacgctg gccgggggct ccgtgcggtg gggtgccctg cacgtgctgg ggacagcagc 961 cctggcggag caccggtcca tgaacccctg ccctgtgcac gatgctggca cgggcaccgt 1021 cttcctcttc ttcatcgcgg tgctgggcca cacgcctgag gccgtgcaga tcgccacggg 1081 aaggaacgcc gcgcgcctct gctgtgtggc cagccgtgac gccggcctct cgtggggcag 1141 cgcccgggac ctcaccgagg aggccatcgg tggtgccgtg caggactggg ccacattcgc 1201 tgtgggtccc ggccacggcg tgcagctgcc ctcaggccgc ctgctggtac ccgcctacac 1261 ctaccgcgtg gaccgccgag agtgttttgg caagatctgc cggaccagcc ctcactcctt 1321 cgccttctac agcgatgacc acggccgcac ctggcgctgt ggaggcctcg tgcccaacct 1381 gcgctcaggc gagtgccagc tggcggcggt ggacggtggg caggccggca gcttcctcta 1441 ctgcaatgcc cggagcccac tgggcagccg tgtgcaggcg ctcagcactg acgagggcac 1501 ctccttcctg cccgcagagc gcgtggcttc cctgcccgag actgcctggg gctgccaggg 1561 cagcatcgtg ggcttcccag cccccgcccc caacaggcca cgggatgaca gttggtcagt 1621 gggccccggg agtcccctcc agcctccact cctcggtcct ggagtccacg aacccccaga 1681 ggaggctgct gtagaccccc gtggaggcca ggtgcctggt gggcccttca gccgtctgca 1741 gcctcggggg gatggcccca ggcagcctgg ccccaggcct ggggtcagtg gggatgtggg 1801 gtcctggacc ctggcactcc ccatgccctt tgctgccccg ccccagagcc ccacgtggct 1861 gctgtactcc cacccagtgg ggcgcagggc tcggctacac atgggtatcc gcctgagcca 1921 gtccccgctg gacccgcgca gctggacaga gccctgggtg atctacgagg gccccagcgg 1981 ctactccgac ctggcgtcca tcgggccggc tcctgagggg ggcctggttt ttgcctgcct 2041 gtacgagagc ggggccagga cctcctatga tgagatttcc ttttgtacat tctccctgcg 2101 tgaggtcctg gagaacgtgc ccgccagccc caagccgccc aaccttgggg acaagcctcg 2161 ggggtgctgc tggccctcct gaCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 2221 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG 2281 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA 2341 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC 2401 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT 2461 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA 2521 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA 2581 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC 2641 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA 2701 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC 2761 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT 2821 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT 2881 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT 2941 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT 3001 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT 3061 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA 3121 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG 3181 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT 3241 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC 3301 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC 3361 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 3421 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 3481 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 3541 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 3601 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 3661 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 3721 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 3781 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 3841 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 3901 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 3961 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 4021 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT //