LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2726..2822) /label=attL2 /note="attL2 (c)" misc_signal complement(2837..2856) /note="T7 Promoter/priming site (c)" primer_bind 2864..2880 /note="M13 Reverse priming site" gene 2993..3802 /note="Kanamycin resistance gene" rep_origin 3923..4596 /note="pUC origin" PCR_frame 669..2726 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2746) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2743) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2734..4599,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2704..2724 /note="TEV site" source 673..2703 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2315 IMAGE:2988086" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_15" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2703 /gene="GLB1" /db_xref="GeneID:2720" /db_xref="HGNC:4298" /db_xref="MIM:230500" CDS 673..2703 /dnas_title="galactosidase, beta 1" /gene="GLB1" /codon_start=1 /product="galactosidase, beta 1" /protein_id="AAH07493.1" /db_xref="GI:13960104" /db_xref="GeneID:2720" /db_xref="HGNC:4298" /db_xref="MIM:230500" /translation="MPGFLVRILLLLLVLLLLGPTRGLRNATQRMFEIDYSRDSFLKD GQPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNAIQTYVPWNFHEPWPGQYQFSEDH DVEYFLRLAHELGLLVILRPGPYICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDK WLGVLLPKMKPLLYQNGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLF TTDGAHKTFLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLDH WGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYD APLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDIL CPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGI PQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTD WTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLP QDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWA PCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV" misc_difference 701..701 /gene="GLB1" /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'L' in cDNA, 'P' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 706..706 /gene="GLB1" /note="'C' in cDNA is 'T' in the human genome; no amino acid change." misc_feature 673..2703 /note="GLB1 coding region" misc_difference 2233..2233 /gene="GLB1" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'R' in cDNA, 'C' in the human genome." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgccggg gttcctggtt cgcatcctcc ttctgctgct ggttctgctg 721 cttctgggcc ctacgcgcgg cttgcgcaat gccacccaga ggatgtttga aattgactat 781 agccgggact ccttcctcaa ggatggccag ccatttcgct acatctcagg aagcattcac 841 tactcccgtg tgccccgctt ctactggaag gaccggctgc tgaagatgaa gatggctggg 901 ctgaacgcca tccagacgta tgtgccctgg aactttcatg agccctggcc aggacagtac 961 cagttttctg aggaccatga tgtggaatat tttcttcggc tggctcatga gctgggactg 1021 ctggttatcc tgaggcccgg gccctacatc tgtgcagagt gggaaatggg aggattacct 1081 gcttggctgc tagagaaaga gtctattctt ctccgctcct ccgacccaga ttacctggca 1141 gctgtggaca agtggttggg agtccttctg cccaagatga agcctctcct ctatcagaat 1201 ggagggccag ttataacagt gcaggttgaa aatgaatatg gcagctactt tgcctgtgat 1261 tttgactacc tgcgcttcct gcagaagcgc tttcgccacc atctggggga tgatgtggtt 1321 ctgtttacca ctgatggagc acataaaaca ttcctgaaat gtggggccct gcagggcctc 1381 tacaccacgg tggactttgg aacaggcagc aacatcacag atgctttcct aagccagagg 1441 aagtgtgagc ccaaaggacc cttgatcaat tctgaattct atactggctg gctagatcac 1501 tggggccaac ctcactccac aatcaagacc gaagcagtgg cttcctccct ctatgatata 1561 cttgcccgtg gggcgagtgt gaacttgtac atgtttatag gtgggaccaa ttttgcctat 1621 tggaatgggg ccaactcacc ctatgcagca cagcccacca gctacgacta tgatgcccca 1681 ctgagtgagg ctggggacct cactgagaag tattttgctc tgcgaaacat catccagaag 1741 tttgaaaaag taccagaagg tcctatccct ccatctacac caaagtttgc atatggaaag 1801 gtcactttgg aaaagttaaa gacagtggga gcagctctgg acattctgtg tccctctggg 1861 cccatcaaaa gcctttatcc cttgacattt atccaggtga aacagcatta tgggtttgtg 1921 ctgtaccgga caacacttcc tcaagattgc agcaacccag cacctctctc ttcacccctc 1981 aatggagtcc acgatcgagc atatgttgct gtggatggga tcccccaggg agtccttgag 2041 cgaaacaatg tgatcactct gaacataaca gggaaagctg gagccactct ggaccttctg 2101 gtagagaaca tgggacgtgt gaactatggt gcatatatca acgattttaa gggtttggtt 2161 tctaacctga ctctcagttc caatatcctc acggactgga cgatctttcc actggacact 2221 gaggatgcag tgcgcagcca cctggggggc tggggacacc gtgacagtgg ccaccatgat 2281 gaagcctggg cccacaactc atccaactac acgctcccgg ccttttatat ggggaacttc 2341 tccattccca gtgggatccc agacttgccc caggacacct ttatccagtt tcctggatgg 2401 accaagggcc aggtctggat taatggcttt aaccttggcc gctattggcc agcccggggc 2461 cctcagttga ccttgtttgt gccccagcac atcctgatga cctcggcccc aaacaccatc 2521 accgtgctgg aactggagtg ggcaccctgc agcagtgatg atccagaact atgtgctgtg 2581 acgttcgtgg acaggccagt tattggctca tctgtgacct acgatcatcc ctccaaacct 2641 gttgaaaaaa gactcatgcc cccacccccg caaaaaaaca aagattcatg gctggaccat 2701 gtagaaaact tgtactttca aggctCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 2761 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 2821 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 2881 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 2941 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 3001 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 3061 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 3121 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 3181 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 3241 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 3301 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 3361 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 3421 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 3481 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 3541 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 3601 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 3661 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 3721 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 3781 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 3841 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 3901 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 3961 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 4021 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 4081 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 4141 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 4201 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 4261 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 4321 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 4381 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 4441 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 4501 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 4561 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //