LOCUS GLB1-pGEn2-DEST 7266 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 5894..6435 /note="WPRE" misc_recomb 5856..5878 /label=attB2 /note="attB2 site" misc_feature complement(6442..6656) /note="BGH pA" misc_feature complement(6744..6873) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" source 3891..5855 /dnas_title="GLB1-pGEn2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2315 IMAGE:2988086" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_15" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3891..5855 /dnas_title="GLB1" /gene="GLB1" /db_xref="GeneID:2720" /db_xref="HGNC:4298" /db_xref="MIM:230500" CDS 3891..5855 /dnas_title="galactosidase, beta 1" /gene="GLB1" /codon_start=1 /product="galactosidase, beta 1" /protein_id="AAH07493.1" /db_xref="GI:13960104" /db_xref="GeneID:2720" /db_xref="HGNC:4298" /db_xref="MIM:230500" /translation="MPGFLVRILLLLLVLLLLGPTRGLRNATQRMFEIDYSRDSFLKD GQPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNAIQTYVPWNFHEPWPGQYQFSEDH DVEYFLRLAHELGLLVILRPGPYICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDK WLGVLLPKMKPLLYQNGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLF TTDGAHKTFLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLDH WGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYD APLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDIL CPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGI PQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTD WTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLP QDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWA PCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV" misc_feature 3891..5855 /note="GLB1 coding region" misc_difference 5382..5382 /dnas_title="GLB1" /gene="GLB1" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'R' in cDNA, 'C' in the human genome." vector join(5864..7266,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..5863 /source="GLB1-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc ttgcgcaatg 3901 ccacccagag gatgtttgaa attgactata gccgggactc cttcctcaag gatggccagc 3961 catttcgcta catctcagga agcattcact actcccgtgt gccccgcttc tactggaagg 4021 accggctgct gaagatgaag atggctgggc tgaacgccat ccagacgtat gtgccctgga 4081 actttcatga gccctggcca ggacagtacc agttttctga ggaccatgat gtggaatatt 4141 ttcttcggct ggctcatgag ctgggactgc tggttatcct gaggcccggg ccctacatct 4201 gtgcagagtg ggaaatggga ggattacctg cttggctgct agagaaagag tctattcttc 4261 tccgctcctc cgacccagat tacctggcag ctgtggacaa gtggttggga gtccttctgc 4321 ccaagatgaa gcctctcctc tatcagaatg gagggccagt tataacagtg caggttgaaa 4381 atgaatatgg cagctacttt gcctgtgatt ttgactacct gcgcttcctg cagaagcgct 4441 ttcgccacca tctgggggat gatgtggttc tgtttaccac tgatggagca cataaaacat 4501 tcctgaaatg tggggccctg cagggcctct acaccacggt ggactttgga acaggcagca 4561 acatcacaga tgctttccta agccagagga agtgtgagcc caaaggaccc ttgatcaatt 4621 ctgaattcta tactggctgg ctagatcact ggggccaacc tcactccaca atcaagaccg 4681 aagcagtggc ttcctccctc tatgatatac ttgcccgtgg ggcgagtgtg aacttgtaca 4741 tgtttatagg tgggaccaat tttgcctatt ggaatggggc caactcaccc tatgcagcac 4801 agcccaccag ctacgactat gatgccccac tgagtgaggc tggggacctc actgagaagt 4861 attttgctct gcgaaacatc atccagaagt ttgaaaaagt accagaaggt cctatccctc 4921 catctacacc aaagtttgca tatggaaagg tcactttgga aaagttaaag acagtgggag 4981 cagctctgga cattctgtgt ccctctgggc ccatcaaaag cctttatccc ttgacattta 5041 tccaggtgaa acagcattat gggtttgtgc tgtaccggac aacacttcct caagattgca 5101 gcaacccagc acctctctct tcacccctca atggagtcca cgatcgagca tatgttgctg 5161 tggatgggat cccccaggga gtccttgagc gaaacaatgt gatcactctg aacataacag 5221 ggaaagctgg agccactctg gaccttctgg tagagaacat gggacgtgtg aactatggtg 5281 catatatcaa cgattttaag ggtttggttt ctaacctgac tctcagttcc aatatcctca 5341 cggactggac gatctttcca ctggacactg aggatgcagt gcgcagccac ctggggggct 5401 ggggacaccg tgacagtggc caccatgatg aagcctgggc ccacaactca tccaactaca 5461 cgctcccggc cttttatatg gggaacttct ccattcccag tgggatccca gacttgcccc 5521 aggacacctt tatccagttt cctggatgga ccaagggcca ggtctggatt aatggcttta 5581 accttggccg ctattggcca gcccggggcc ctcagttgac cttgtttgtg ccccagcaca 5641 tcctgatgac ctcggcccca aacaccatca ccgtgctgga actggagtgg gcaccctgca 5701 gcagtgatga tccagaacta tgtgctgtga cgttcgtgga caggccagtt attggctcat 5761 ctgtgaccta cgatcatccc tccaaacctg ttgaaaaaag actcatgccc ccacccccgc 5821 aaaaaaacaa agattcatgg ctggaccatg tatgaCCAGC TTTCTTGTAC AAAGTGGTTG 5881 ATAGCTTGGA TCCAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT 5941 TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC 6001 TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT 6061 TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA 6121 CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC 6181 TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC 6241 AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC TGACGTCCTT 6301 TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT 6361 CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC 6421 TCTTCCGCGT CTTCGAGATC TGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT 6481 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA 6541 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG 6601 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGACTCG 6661 AGTTAAGGGC GAATTGCCGA TTAGGATCTT CCTAGAGCAT GGCTACGTAG ATAAGTAGCA 6721 TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT 6781 GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC 6841 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCCTTAAT TAAGAGCTCA TGGCGCGCCT 6901 AGGCCTTGAC GGCCTTCCGC CAATTCGCCC TATAGTGAGT CGTATTACGT CGCGCTCACT 6961 GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT 7021 TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGAAACGCC 7081 CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGAGCG CCCTGTAGCG GCCACTCAAC 7141 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 7201 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA 7261 ATTTAG //