LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2660..2756) /label=attL2 /note="attL2 (c)" misc_signal complement(2771..2790) /note="T7 Promoter/priming site (c)" primer_bind 2798..2814 /note="M13 Reverse priming site" gene 2927..3736 /note="Kanamycin resistance gene" rep_origin 3857..4530 /note="pUC origin" vector join(2668..4533,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2653 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="DNA36992" gene 692..2653 /locus_tag="UNQ229" CDS 692..2653 /dnas_title="APKK229" /locus_tag="UNQ229" /note="PRO262" /codon_start=1 /product="APKK229" /protein_id="AAQ89002.1" /db_xref="GI:37182400" /translation="MAPKKLSCLRSLLLPLSLTLLLPQADTRSFVVDRGHDRFLLDGA PFRYVSGSLHYFRVPRVLWADRLLKMRWSGLNAIQFYVPWNYHEPQPGVYNFNGSRDL IAFLNEAALANLLVILRPGPYICAEWEMGGLPSWLLRKPEIHLRTSDPDFLAAVDSWF KVLLPKIYPWLYHNGGNIISIQVENEYGSYRACDFSYMRHLAGLFRALLGEKILLFTT DGPEGLKCGSLRGLYTTVDFGPADNMTKIFTLLRKYEPHGPLVNSEYYTGWLDYWGQN HSTRSVSAVTKGLENMLKLGASVNMYMFHGGTNFGYWNGADKKGRFLPITTSYDYDAP ISEAGDPTPKLFALRDVISKFQEVPLGPLPPPSPKMMLGPVTLHLVGHLLAFLDLLCP RGPIHSILPMTFEAVKQDHGFMLYRTYMTHTIFEPTPFWVPNNGVHDRAYVMVDGVFQ GVVERNMRDKLFLTGKLGSKLDILVENMGRLSFGSNSSDFKGLLKPPILGQTILTQWM MFPLKIDNLVKWWFPLQLPKWPYPQAPSGPTFYSKTFPILGSVGDTFLYLPGWTKGQV WINGFNLGRYWTKQGPQQTLYVPRFLLFPRGALNKITLLELEDVPLQPQVQFLDKPIL NSTSTLHRTHINSLSADTLSASEPMELSGH" misc_feature 692..2653 /note="GLB1L coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg catggctccc aagaagctgt cctgccttcg 721 ttccctgctg ctgccgctca gcctgacgct actgctgccc caggcagaca ctcggtcgtt 781 cgtagtggat aggggtcatg accggtttct cctagacggg gccccgttcc gctatgtgtc 841 tggcagcctg cactactttc gggtaccgcg ggtgctttgg gccgaccggc ttttgaagat 901 gcgatggagc ggcctcaacg ccatacagtt ttatgtgccc tggaactacc acgagccaca 961 gcctggggtc tataacttta atggcagccg ggacctcatt gcctttctga atgaggcagc 1021 tctagcgaac ctgttggtca tactgagacc aggaccttac atctgtgcag agtgggagat 1081 ggggggtctc ccatcctggt tgcttcgaaa acctgaaatt catctaagaa cctcagatcc 1141 agacttcctt gccgcagtgg actcctggtt caaggtcttg ctgcccaaga tatatccatg 1201 gctttatcac aatgggggca acatcattag cattcaggtg gagaatgaat atggtagcta 1261 cagagcctgt gacttcagct acatgaggca cttggctggg ctcttccgtg cactgctagg 1321 agaaaagatc ttgctcttca ccacagatgg gcctgaagga ctcaagtgtg gctccctccg 1381 gggactctat accactgtag attttggccc agctgacaac atgaccaaaa tctttaccct 1441 gcttcggaag tatgaacccc atgggccatt ggtaaactct gagtactaca caggctggct 1501 ggattactgg ggccagaatc actccacacg gtctgtgtca gctgtaacca aaggactaga 1561 gaacatgctc aagttgggag ccagtgtgaa catgtacatg ttccatggag gtaccaactt 1621 tggatattgg aatggtgccg ataagaaggg acgcttcctt ccgattacta ccagctatga 1681 ctatgatgca cctatatctg aagcagggga ccccacacct aagctttttg ctcttcgaga 1741 tgtcatcagc aagttccagg aagttccttt gggaccttta cctcccccga gccccaagat 1801 gatgcttgga cctgtgactc tgcacctggt tgggcattta ctggctttcc tagacttgct 1861 ttgcccccgt gggcccattc attcaatctt gccaatgacc tttgaggctg tcaagcagga 1921 ccatggcttc atgttgtacc gaacctatat gacccatacc atttttgagc caacaccatt 1981 ctgggtgcca aataatggag tccatgaccg tgcctatgtg atggtggatg gggtgttcca 2041 gggtgttgtg gagcgaaata tgagagacaa actatttttg acggggaaac tggggtccaa 2101 actggatatc ttggtggaga acatggggag gctcagcttt gggtctaaca gcagtgactt 2161 caagggcctg ttgaagccac caattctggg gcaaacaatc cttacccagt ggatgatgtt 2221 ccctctgaaa attgataacc ttgtgaagtg gtggtttccc ctccagttgc caaaatggcc 2281 atatcctcaa gctccttctg gccccacatt ctactccaaa acatttccaa ttttaggctc 2341 agttggggac acatttctat atctacctgg atggaccaag ggccaagtct ggatcaatgg 2401 gtttaacttg ggccggtact ggacaaagca ggggccacaa cagaccctct acgtgccaag 2461 attcctgctg tttcctaggg gagccctcaa caaaattaca ttgctggaac tagaagatgt 2521 acctctccag ccccaagtcc aatttttgga taagcctatc ctcaatagca ctagtacttt 2581 gcacaggaca catatcaatt ccctttcagc tgatacactg agtgcctctg aaccaatgga 2641 gttaagtggg cacTAGGACC CAGCTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG 2701 CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA 2761 TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT 2821 CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAATAA TATCATCATG 2881 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA 2941 ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA 3001 ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC 3061 CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA 3121 TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT 3181 TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT 3241 CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT 3301 CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT 3361 TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA 3421 TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC 3481 ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA 3541 CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA 3601 GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT 3661 TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT 3721 CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT 3781 GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT 3841 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 3901 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 3961 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 4021 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 4081 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 4141 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 4201 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 4261 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 4321 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 4381 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 4441 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 4501 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT //