LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2603..2699) /label=attL2 /note="attL2 (c)" misc_signal complement(2714..2733) /note="T7 Promoter/priming site (c)" primer_bind 2741..2757 /note="M13 Reverse priming site" gene 2870..3679 /note="Kanamycin resistance gene" rep_origin 3800..4473 /note="pUC origin" PCR_frame 669..2603 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2623) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2620) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2611..4476,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2581..2601 /note="TEV site" source 673..2580 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:9706 IMAGE:3849776" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_65" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..2580 /gene="GLB1L2" /gene_synonym=MST114 /gene_synonym=MSTP114 /db_xref="GeneID:89944" /db_xref="HGNC:25129" CDS 673..2580 /dnas_title="galactosidase, beta 1-like 2" /gene="GLB1L2" /gene_synonym=MST114 /gene_synonym=MSTP114 /codon_start=1 /product="galactosidase, beta 1-like 2" /protein_id="AAH40641.1" /db_xref="GI:26251705" /db_xref="GeneID:89944" /db_xref="HGNC:25129" /translation="MTTWSLRRRPARTLGLLLLVVLGFLVLRRLDWSTLVPLRLRHRQ LGLQAKGWNFMLEDSTFWIFGGSIHYFRVPREYWRDRLLKMKACGLNTLTTYVPWNLH EPERGKFDFSGNLDLEAFVLMAAEIGLWVILRPGPYICSEMDLGGLPSWLLQDPGMRL RTTYKGFTEAVDLYFDHLMSRVVPLQYKRGGPIIAVQVENEYGSYNKDPAYMPYVKKA LEDRGIVELLLTSDNKDGLSKGIVQGVLATINLQSTHELQLLTTFLFNVQGTQPKMVM EYWTGWFDSWGGPHNILDSSEVLKTVSAIVDAGSSINLYMFHGGTNFGFMNGAMHFHD YKSDVTSYDYDAVLTEAGDYTAKYMKLRDFFGSISGIPLPPPPDLLPKMPYEPLTPVL YLSLWDALKYLGEPIKSEKPINMENLPVNGGNGQSFGYILYETSITSSGILSGHVHDR GQVFVNTVSIGFLDYKTTKIAVPLIQGYTVLRILVENRGRVNYGENIDDQRKGLIGNL YLNDSPLKNFRIYSLDMKKSFFQRFGLDKWSSLPETPTLPAFFLGSLSISSTPCDTFL KLEGWEKGVVFINGQNLGRYWNIGPQKTLYLPGPWLSSGINQVIVFEETMAGPALQFT ETPHLGRNQYIK" misc_feature 673..2580 /note="GLB1L2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgaccac gtggagcctc cggcggaggc cggcccgcac gctgggactc 721 ctgctgctgg tcgtcttggg cttcctggtg ctccgcaggc tggactggag caccctggtc 781 cctctgcggc tccgccatcg acagctgggg ctgcaggcca agggctggaa cttcatgctg 841 gaggattcca ccttctggat cttcgggggc tccatccact atttccgtgt gcccagggag 901 tactggaggg accgcctgct gaagatgaag gcctgtggct tgaacaccct caccacctat 961 gttccgtgga acctgcatga gccagaaaga ggcaaatttg acttctctgg gaacctggac 1021 ctggaggcct tcgtcctgat ggccgcagag atcgggctgt gggtgattct gcgtccaggc 1081 ccctacatct gcagtgagat ggacctcggg ggcttgccca gctggctact ccaagaccct 1141 ggcatgaggc tgaggacaac ttacaagggc ttcaccgaag cagtggacct ttattttgac 1201 cacctgatgt ccagggtggt gccactccag tacaagcgtg ggggacctat cattgccgtg 1261 caggtggaga atgaatatgg ttcctataat aaagaccccg catacatgcc ctacgtcaag 1321 aaggcactgg aggaccgtgg cattgtggaa ctgctcctga cttcagacaa caaggatggg 1381 ctgagcaagg ggattgtcca gggagtcttg gccaccatca acttgcagtc aacacacgag 1441 ctgcagctac tgaccacctt tctcttcaac gtccagggga ctcagcccaa gatggtgatg 1501 gagtactgga cggggtggtt tgactcgtgg ggaggccctc acaatatctt ggattcttct 1561 gaggttttga aaaccgtgtc tgccattgtg gacgccggct cctccatcaa cctctacatg 1621 ttccacggag gcaccaactt tggcttcatg aatggagcca tgcacttcca tgactacaag 1681 tcagatgtca ccagctatga ctatgatgct gtgctgacag aagccggcga ttacacggcc 1741 aagtacatga agcttcgaga cttcttcggc tccatctcag gcatccctct ccctccccca 1801 cctgaccttc ttcccaagat gccgtatgag cccttaacgc cagtcttgta cctgtctctg 1861 tgggacgccc tcaagtacct gggggagcca atcaagtctg aaaagcccat caacatggag 1921 aacctgccag tcaatggggg aaatggacag tccttcgggt acattctcta tgagaccagc 1981 atcacctcgt ctggcatcct cagtggccac gtgcatgatc gggggcaggt gtttgtgaac 2041 acagtatcca taggattctt ggactacaag acaacgaaga ttgctgtccc cctgatccag 2101 ggttacaccg tgctgaggat cttggtggag aatcgtgggc gagtcaacta tggggagaat 2161 attgatgacc agcgcaaagg cttaattgga aatctctatc tgaatgattc acccctgaaa 2221 aacttcagaa tctatagcct ggatatgaag aagagcttct ttcagaggtt cggcctggac 2281 aaatggagtt ccctcccaga aacacccaca ttacctgctt tcttcttggg tagcttgtcc 2341 atcagctcca ccccttgtga cacctttctg aagctggagg gctgggagaa gggggttgta 2401 ttcatcaatg gccagaacct tggacgttac tggaacattg gaccccagaa gacgctttac 2461 ctcccaggtc cctggttgag cagcggaatc aaccaggtca tcgtttttga ggagacgatg 2521 gcgggccctg cattacagtt cacggaaacc ccccacctgg gcaggaacca gtacattaag 2581 gaaaacttgt actttcaagg ctCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 2641 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG 2701 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA 2761 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC 2821 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT 2881 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA 2941 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA 3001 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC 3061 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA 3121 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC 3181 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT 3241 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT 3301 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT 3361 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT 3421 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT 3481 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA 3541 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG 3601 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT 3661 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC 3721 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC 3781 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 3841 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 3901 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 3961 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 4021 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 4081 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 4141 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 4201 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 4261 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 4321 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 4381 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 4441 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT //