LOCUS GLB1L2-pGEc1-DES 6403 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5881..6010) /note="3'-ITR" misc_feature complement(5579..5793) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5031..5572 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_recomb 4936..4958 /label=attB2 /note="attB2 site" misc_feature 4968..4991 /note="8xHis Tag" misc_feature 4995..5018 /note="StrepTag" PCR_frame 3002..4936 /note="Reading Frame" /set="Set 1" source 3006..4913 /dnas_title="GLB1L2-pGEc1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:9706 IMAGE:3849776" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_65" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4913 /dnas_title="GLB1L2" /gene="GLB1L2" /gene_synonym="MST114" /gene_synonym="MSTP114" /db_xref="GeneID:89944" /db_xref="HGNC:25129" CDS 3006..4913 /dnas_title="galactosidase, beta 1-like 2" /gene="GLB1L2" /gene_synonym="MST114" /gene_synonym="MSTP114" /codon_start=1 /product="galactosidase, beta 1-like 2" /protein_id="AAH40641.1" /db_xref="GI:26251705" /db_xref="GeneID:89944" /db_xref="HGNC:25129" /translation="MTTWSLRRRPARTLGLLLLVVLGFLVLRRLDWSTLVPLRLRHRQ LGLQAKGWNFMLEDSTFWIFGGSIHYFRVPREYWRDRLLKMKACGLNTLTTYVPWNLH EPERGKFDFSGNLDLEAFVLMAAEIGLWVILRPGPYICSEMDLGGLPSWLLQDPGMRL RTTYKGFTEAVDLYFDHLMSRVVPLQYKRGGPIIAVQVENEYGSYNKDPAYMPYVKKA LEDRGIVELLLTSDNKDGLSKGIVQGVLATINLQSTHELQLLTTFLFNVQGTQPKMVM EYWTGWFDSWGGPHNILDSSEVLKTVSAIVDAGSSINLYMFHGGTNFGFMNGAMHFHD YKSDVTSYDYDAVLTEAGDYTAKYMKLRDFFGSISGIPLPPPPDLLPKMPYEPLTPVL YLSLWDALKYLGEPIKSEKPINMENLPVNGGNGQSFGYILYETSITSSGILSGHVHDR GQVFVNTVSIGFLDYKTTKIAVPLIQGYTVLRILVENRGRVNYGENIDDQRKGLIGNL YLNDSPLKNFRIYSLDMKKSFFQRFGLDKWSSLPETPTLPAFFLGSLSISSTPCDTFL KLEGWEKGVVFINGQNLGRYWNIGPQKTLYLPGPWLSSGINQVIVFEETMAGPALQFT ETPHLGRNQYIK" misc_feature 3006..4913 /note="GLB1L2 coding region" misc_feature 4914..4934 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4943) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4943) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4944..6403,1..2991) /source="pGEc1-DEST" /type="Destination Vector" clone 2992..4943 /source="GLB1L2-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgac cacgtggagc ctccggcgga ggccggcccg cacgctggga ctcctgctgc 3061 tggtcgtctt gggcttcctg gtgctccgca ggctggactg gagcaccctg gtccctctgc 3121 ggctccgcca tcgacagctg gggctgcagg ccaagggctg gaacttcatg ctggaggatt 3181 ccaccttctg gatcttcggg ggctccatcc actatttccg tgtgcccagg gagtactgga 3241 gggaccgcct gctgaagatg aaggcctgtg gcttgaacac cctcaccacc tatgttccgt 3301 ggaacctgca tgagccagaa agaggcaaat ttgacttctc tgggaacctg gacctggagg 3361 ccttcgtcct gatggccgca gagatcgggc tgtgggtgat tctgcgtcca ggcccctaca 3421 tctgcagtga gatggacctc gggggcttgc ccagctggct actccaagac cctggcatga 3481 ggctgaggac aacttacaag ggcttcaccg aagcagtgga cctttatttt gaccacctga 3541 tgtccagggt ggtgccactc cagtacaagc gtgggggacc tatcattgcc gtgcaggtgg 3601 agaatgaata tggttcctat aataaagacc ccgcatacat gccctacgtc aagaaggcac 3661 tggaggaccg tggcattgtg gaactgctcc tgacttcaga caacaaggat gggctgagca 3721 aggggattgt ccagggagtc ttggccacca tcaacttgca gtcaacacac gagctgcagc 3781 tactgaccac ctttctcttc aacgtccagg ggactcagcc caagatggtg atggagtact 3841 ggacggggtg gtttgactcg tggggaggcc ctcacaatat cttggattct tctgaggttt 3901 tgaaaaccgt gtctgccatt gtggacgccg gctcctccat caacctctac atgttccacg 3961 gaggcaccaa ctttggcttc atgaatggag ccatgcactt ccatgactac aagtcagatg 4021 tcaccagcta tgactatgat gctgtgctga cagaagccgg cgattacacg gccaagtaca 4081 tgaagcttcg agacttcttc ggctccatct caggcatccc tctccctccc ccacctgacc 4141 ttcttcccaa gatgccgtat gagcccttaa cgccagtctt gtacctgtct ctgtgggacg 4201 ccctcaagta cctgggggag ccaatcaagt ctgaaaagcc catcaacatg gagaacctgc 4261 cagtcaatgg gggaaatgga cagtccttcg ggtacattct ctatgagacc agcatcacct 4321 cgtctggcat cctcagtggc cacgtgcatg atcgggggca ggtgtttgtg aacacagtat 4381 ccataggatt cttggactac aagacaacga agattgctgt ccccctgatc cagggttaca 4441 ccgtgctgag gatcttggtg gagaatcgtg ggcgagtcaa ctatggggag aatattgatg 4501 accagcgcaa aggcttaatt ggaaatctct atctgaatga ttcacccctg aaaaacttca 4561 gaatctatag cctggatatg aagaagagct tctttcagag gttcggcctg gacaaatgga 4621 gttccctccc agaaacaccc acattacctg ctttcttctt gggtagcttg tccatcagct 4681 ccaccccttg tgacaccttt ctgaagctgg agggctggga gaagggggtt gtattcatca 4741 atggccagaa ccttggacgt tactggaaca ttggacccca gaagacgctt tacctcccag 4801 gtccctggtt gagcagcgga atcaaccagg tcatcgtttt tgaggagacg atggcgggcc 4861 ctgcattaca gttcacggaa accccccacc tgggcaggaa ccagtacatt aaggaaaact 4921 tgtactttca aggctCCAGC TTTCTTGTAC AAAGTGGTGA TGGCCGCCAT CATCATCATC 4981 ATCATCATCA TGCGTGGAGC CACCCGCAGT TCGAAAAATA ATAAGGATCC AATCAACCTC 5041 TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC 5101 TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA 5161 TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG 5221 TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA 5281 TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG 5341 CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG 5401 ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG 5461 CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG 5521 ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGAGATCTGC 5581 CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT 5641 GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA 5701 TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA 5761 GGATTGGGAA GACAATAGCA GGCATGCTGG GGACTCGAGT TAAGGGCGAA TTGCCGATTA 5821 GGATCTTCCT AGAGCATGGC TACGTAGATA AGTAGCATGG CGGGTTAATC ATTAACTACA 5881 AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG 5941 CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC 6001 GAGCGCGCAG CCTTAATTAA GAGCTCATGG CGCGCCTAGG CCTTGACGGC CTTCCGCCAA 6061 TTCGCCCTAT AGTGAGTCGT ATTACGTCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG 6121 ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA 6181 GCTGGCGTAA TAGCGAAGAG GCCCGCACCG AAACGCCCTT CCCAACAGTT GCGCAGCCTG 6241 AATGGCGAAT GGGAGCGCCC TGTAGCGGCC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA 6301 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 6361 ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TAG //