LOCUS GLB1L2-pGEc2-DES 7129 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6607..6736) /note="3'-ITR" misc_feature complement(6305..6519) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5757..6298 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4936..4958 /note="attB2" misc_feature 5736..5741 /note="BstB1 site" misc_feature 4959..5654 /note="Super GFP" misc_feature 5655..5705 /note="AviTag" misc_feature 5706..5729 /note="His Tag" PCR_frame 3002..4936 /note="Reading Frame" /set="Set 1" source 3006..4913 /dnas_title="GLB1L2-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:9706 IMAGE:3849776" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_65" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4913 /dnas_title="GLB1L2" /gene="GLB1L2" /gene_synonym="MST114" /gene_synonym="MSTP114" /db_xref="GeneID:89944" /db_xref="HGNC:25129" CDS 3006..4913 /dnas_title="galactosidase, beta 1-like 2" /gene="GLB1L2" /gene_synonym="MST114" /gene_synonym="MSTP114" /codon_start=1 /product="galactosidase, beta 1-like 2" /protein_id="AAH40641.1" /db_xref="GI:26251705" /db_xref="GeneID:89944" /db_xref="HGNC:25129" /translation="MTTWSLRRRPARTLGLLLLVVLGFLVLRRLDWSTLVPLRLRHRQ LGLQAKGWNFMLEDSTFWIFGGSIHYFRVPREYWRDRLLKMKACGLNTLTTYVPWNLH EPERGKFDFSGNLDLEAFVLMAAEIGLWVILRPGPYICSEMDLGGLPSWLLQDPGMRL RTTYKGFTEAVDLYFDHLMSRVVPLQYKRGGPIIAVQVENEYGSYNKDPAYMPYVKKA LEDRGIVELLLTSDNKDGLSKGIVQGVLATINLQSTHELQLLTTFLFNVQGTQPKMVM EYWTGWFDSWGGPHNILDSSEVLKTVSAIVDAGSSINLYMFHGGTNFGFMNGAMHFHD YKSDVTSYDYDAVLTEAGDYTAKYMKLRDFFGSISGIPLPPPPDLLPKMPYEPLTPVL YLSLWDALKYLGEPIKSEKPINMENLPVNGGNGQSFGYILYETSITSSGILSGHVHDR GQVFVNTVSIGFLDYKTTKIAVPLIQGYTVLRILVENRGRVNYGENIDDQRKGLIGNL YLNDSPLKNFRIYSLDMKKSFFQRFGLDKWSSLPETPTLPAFFLGSLSISSTPCDTFL KLEGWEKGVVFINGQNLGRYWNIGPQKTLYLPGPWLSSGINQVIVFEETMAGPALQFT ETPHLGRNQYIK" misc_feature 3006..4913 /note="GLB1L2 coding region" misc_feature 4914..4934 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4943) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4943) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4944..7129,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4943 /source="GLB1L2-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgac cacgtggagc ctccggcgga ggccggcccg cacgctggga ctcctgctgc 3061 tggtcgtctt gggcttcctg gtgctccgca ggctggactg gagcaccctg gtccctctgc 3121 ggctccgcca tcgacagctg gggctgcagg ccaagggctg gaacttcatg ctggaggatt 3181 ccaccttctg gatcttcggg ggctccatcc actatttccg tgtgcccagg gagtactgga 3241 gggaccgcct gctgaagatg aaggcctgtg gcttgaacac cctcaccacc tatgttccgt 3301 ggaacctgca tgagccagaa agaggcaaat ttgacttctc tgggaacctg gacctggagg 3361 ccttcgtcct gatggccgca gagatcgggc tgtgggtgat tctgcgtcca ggcccctaca 3421 tctgcagtga gatggacctc gggggcttgc ccagctggct actccaagac cctggcatga 3481 ggctgaggac aacttacaag ggcttcaccg aagcagtgga cctttatttt gaccacctga 3541 tgtccagggt ggtgccactc cagtacaagc gtgggggacc tatcattgcc gtgcaggtgg 3601 agaatgaata tggttcctat aataaagacc ccgcatacat gccctacgtc aagaaggcac 3661 tggaggaccg tggcattgtg gaactgctcc tgacttcaga caacaaggat gggctgagca 3721 aggggattgt ccagggagtc ttggccacca tcaacttgca gtcaacacac gagctgcagc 3781 tactgaccac ctttctcttc aacgtccagg ggactcagcc caagatggtg atggagtact 3841 ggacggggtg gtttgactcg tggggaggcc ctcacaatat cttggattct tctgaggttt 3901 tgaaaaccgt gtctgccatt gtggacgccg gctcctccat caacctctac atgttccacg 3961 gaggcaccaa ctttggcttc atgaatggag ccatgcactt ccatgactac aagtcagatg 4021 tcaccagcta tgactatgat gctgtgctga cagaagccgg cgattacacg gccaagtaca 4081 tgaagcttcg agacttcttc ggctccatct caggcatccc tctccctccc ccacctgacc 4141 ttcttcccaa gatgccgtat gagcccttaa cgccagtctt gtacctgtct ctgtgggacg 4201 ccctcaagta cctgggggag ccaatcaagt ctgaaaagcc catcaacatg gagaacctgc 4261 cagtcaatgg gggaaatgga cagtccttcg ggtacattct ctatgagacc agcatcacct 4321 cgtctggcat cctcagtggc cacgtgcatg atcgggggca ggtgtttgtg aacacagtat 4381 ccataggatt cttggactac aagacaacga agattgctgt ccccctgatc cagggttaca 4441 ccgtgctgag gatcttggtg gagaatcgtg ggcgagtcaa ctatggggag aatattgatg 4501 accagcgcaa aggcttaatt ggaaatctct atctgaatga ttcacccctg aaaaacttca 4561 gaatctatag cctggatatg aagaagagct tctttcagag gttcggcctg gacaaatgga 4621 gttccctccc agaaacaccc acattacctg ctttcttctt gggtagcttg tccatcagct 4681 ccaccccttg tgacaccttt ctgaagctgg agggctggga gaagggggtt gtattcatca 4741 atggccagaa ccttggacgt tactggaaca ttggacccca gaagacgctt tacctcccag 4801 gtccctggtt gagcagcgga atcaaccagg tcatcgtttt tgaggagacg atggcgggcc 4861 ctgcattaca gttcacggaa accccccacc tgggcaggaa ccagtacatt aaggaaaact 4921 tgtactttca aggctCCAGC TTTCTTGTAC AAAGTGGTAT GAGCAAGGGC GAGGAACTGT 4981 TCACCGGCGT GGTGCCCATC CTGGTGGAAC TGGATGGCGA CGTGAACGGC CACAAGTTCT 5041 CTGTGCGGGG AGAGGGCGAA GGCGACGCCA CAAATGGCAA GCTGACCCTG AAGTTCATCT 5101 GCACCACCGG CAAGCTGCCC GTGCCTTGGC CTACCCTCGT GACCACACTG ACCTACGGCG 5161 TGCAGTGCTT CAGCAGATAC CCCGACCATA TGAAGCGGCA CGACTTCTTC AAGAGCGCCA 5221 TGCCCGAGGG CTACGTGCAG GAACGGACCA TCAGCTTCAA GGACGACGGC ACCTACAAGA 5281 CCAGAGCCGA AGTGAAGTTC GAGGGCGACA CCCTCGTGAA CCGGATCGAG CTGAAGGGCA 5341 TCGATTTCAA AGAGGACGGC AACATCCTGG GCCACAAGCT GGAGTACAAC TTCAACAGCC 5401 ACAACGTGTA CATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC TTCAAGATCC 5461 GGCACAACGT GGAAGATGGC AGCGTGCAGC TGGCCGACCA CTACCAGCAG AACACCCCCA 5521 TCGGAGATGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG AGCGTGCTGA 5581 GCAAGGACCC CAACGAGAAG CGGGACCACA TGGTGCTGCT GGAATTCGTG ACCGCCGCTG 5641 GCATCACCCA CGGCATGAGC GGCCTGAACG ACATCTTCGA GGCCCAGAAA ATCGAGTGGC 5701 ACGAGCACCA CCATCATCAC CATCACCACT GATGATTCGA AAAATAATAA GGATCCAATC 5761 AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT 5821 TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG 5881 CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC 5941 CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT 6001 GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG 6061 CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG 6121 GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT 6181 GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC 6241 CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGAG 6301 ATCTGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC 6361 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC 6421 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA 6481 GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAC TCGAGTTAAG GGCGAATTGC 6541 CGATTAGGAT CTTCCTAGAG CATGGCTACG TAGATAAGTA GCATGGCGGG TTAATCATTA 6601 ACTACAAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC TCTGCGCGCT CGCTCGCTCA 6661 CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA 6721 GCGAGCGAGC GCGCAGCCTT AATTAAGAGC TCATGGCGCG CCTAGGCCTT GACGGCCTTC 6781 CGCCAATTCG CCCTATAGTG AGTCGTATTA CGTCGCGCTC ACTGGCCGTC GTTTTACAAC 6841 GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT 6901 TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGAAAC GCCCTTCCCA ACAGTTGCGC 6961 AGCCTGAATG GCGAATGGGA GCGCCCTGTA GCGGCCACTC AACCCTATCT CGGTCTATTC 7021 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA 7081 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAG //