LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2507..2603) /label=attL2 /note="attL2 (c)" misc_signal complement(2618..2637) /note="T7 Promoter/priming site (c)" primer_bind 2645..2661 /note="M13 Reverse priming site" gene 2774..3583 /note="Kanamycin resistance gene" rep_origin 3704..4377 /note="pUC origin" vector join(2515..4380,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2506 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:9706 IMAGE:3849776" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_65" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..2506 /gene="GLB1L2" /gene_synonym=MST114 /gene_synonym=MSTP114 /db_xref="GeneID:89944" /db_xref="HGNC:25129" CDS 692..2506 /dnas_title="galactosidase, beta 1-like 2" /gene="GLB1L2" /gene_synonym=MST114 /gene_synonym=MSTP114 /codon_start=1 /product="galactosidase, beta 1-like 2" /protein_id="AAH40641.1" /db_xref="GI:26251705" /db_xref="GeneID:89944" /db_xref="HGNC:25129" /translation="MTTWSLRRRPARTLGLLLLVVLGFLVLRRLDWSTLVPLRLRHRQ LGLQAKGWNFMLEDSTFWIFGGSIHYFRVPREYWRDRLLKMKACGLNTLTTYVPWNLH EPERGKFDFSGNLDLEAFVLMAAEIGLWVILRPGPYICSEMDLGGLPSWLLQDPGMRL RTTYKGFTEAVDLYFDHLMSRVVPLQYKRGGPIIAVQVENEYGSYNKDPAYMPYVKKA LEDRGIVELLLTSDNKDGLSKGIVQGVLATINLQSTHELQLLTTFLFNVQGTQPKMVM EYWTGWFDSWGGPHNILDSSEVLKTVSAIVDAGSSINLYMFHGGTNFGFMNGAMHFHD YKSDVTSYDYDAVLTEAGDYTAKYMKLRDFFGSISGIPLPPPPDLLPKMPYEPLTPVL YLSLWDALKYLGEPIKSEKPINMENLPVNGGNGQSFGYILYETSITSSGILSGHVHDR GQVFVNTVSIGFLDYKTTKIAVPLIQGYTVLRILVENRGRVNYGENIDDQRKGLIGNL YLNDSPLKNFRIYSLDMKKSFFQRFGLDKWSSLPETPTLPAFFLGSLSISSTPCDTFL KLEGWEKGVVFINGQNLGRYWNIGPQKTLYLPGPWLSSGINQVIVFEETMAGPALQFT ETPHLGRNQYIK" misc_feature 692..2506 /note="GLB1L2" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cagcaccctg gtccctctgc ggctccgcca 721 tcgacagctg gggctgcagg ccaagggctg gaacttcatg ctggaggatt ccaccttctg 781 gatcttcggg ggctccatcc actatttccg tgtgcccagg gagtactgga gggaccgcct 841 gctgaagatg aaggcctgtg gcttgaacac cctcaccacc tatgttccgt ggaacctgca 901 tgagccagaa agaggcaaat ttgacttctc tgggaacctg gacctggagg ccttcgtcct 961 gatggccgca gagatcgggc tgtgggtgat tctgcgtcca ggcccctaca tctgcagtga 1021 gatggacctc gggggcttgc ccagctggct actccaagac cctggcatga ggctgaggac 1081 aacttacaag ggcttcaccg aagcagtgga cctttatttt gaccacctga tgtccagggt 1141 ggtgccactc cagtacaagc gtgggggacc tatcattgcc gtgcaggtgg agaatgaata 1201 tggttcctat aataaagacc ccgcatacat gccctacgtc aagaaggcac tggaggaccg 1261 tggcattgtg gaactgctcc tgacttcaga caacaaggat gggctgagca aggggattgt 1321 ccagggagtc ttggccacca tcaacttgca gtcaacacac gagctgcagc tactgaccac 1381 ctttctcttc aacgtccagg ggactcagcc caagatggtg atggagtact ggacggggtg 1441 gtttgactcg tggggaggcc ctcacaatat cttggattct tctgaggttt tgaaaaccgt 1501 gtctgccatt gtggacgccg gctcctccat caacctctac atgttccacg gaggcaccaa 1561 ctttggcttc atgaatggag ccatgcactt ccatgactac aagtcagatg tcaccagcta 1621 tgactatgat gctgtgctga cagaagccgg cgattacacg gccaagtaca tgaagcttcg 1681 agacttcttc ggctccatct caggcatccc tctccctccc ccacctgacc ttcttcccaa 1741 gatgccgtat gagcccttaa cgccagtctt gtacctgtct ctgtgggacg ccctcaagta 1801 cctgggggag ccaatcaagt ctgaaaagcc catcaacatg gagaacctgc cagtcaatgg 1861 gggaaatgga cagtccttcg ggtacattct ctatgagacc agcatcacct cgtctggcat 1921 cctcagtggc cacgtgcatg atcgggggca ggtgtttgtg aacacagtat ccataggatt 1981 cttggactac aagacaacga agattgctgt ccccctgatc cagggttaca ccgtgctgag 2041 gatcttggtg gagaatcgtg ggcgagtcaa ctatggggag aatattgatg accagcgcaa 2101 aggcttaatt ggaaatctct atctgaatga ttcacccctg aaaaacttca gaatctatag 2161 cctggatatg aagaagagct tctttcagag gttcggcctg gacaaatgga gttccctccc 2221 agaaacaccc acattacctg ctttcttctt gggtagcttg tccatcagct ccaccccttg 2281 tgacaccttt ctgaagctgg agggctggga gaagggggtt gtattcatca atggccagaa 2341 ccttggacgt tactggaaca ttggacccca gaagacgctt tacctcccag gtccctggtt 2401 gagcagcgga atcaaccagg tcatcgtttt tgaggagacg atggcgggcc ctgcattaca 2461 gttcacggaa accccccacc tgggcaggaa ccagtacatt aagtgaCCAG CTTTCTTGTA 2521 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT 2581 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA 2641 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT 2701 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA 2761 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA 2821 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC 2881 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA 2941 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT 3001 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC 3061 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 3121 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA 3181 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA 3241 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT 3301 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA 3361 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 3421 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA 3481 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT 3541 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG 3601 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA 3661 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 3721 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 3781 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 3841 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG 3901 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 3961 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 4021 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 4081 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 4141 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 4201 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 4261 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 4321 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT //