LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2267..2363) /label=attL2 /note="attL2 (c)" misc_signal complement(2378..2397) /note="T7 Promoter/priming site (c)" primer_bind 2405..2421 /note="M13 Reverse priming site" gene 2534..3343 /note="Kanamycin resistance gene" rep_origin 3464..4137 /note="pUC origin" PCR_frame 669..2267 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2287) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2284) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2275..4140,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2245..2265 /note="TEV site" source 673..2244 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:129621 IMAGE:40006501" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_283" /note="Vector: pCR-Blunt II-TOPO with reversed insert; Clone identification sequence tag: ATACAATA sequenced from the forward primer" gene 673..2244 /gene="TREH" /gene_synonym=TRE /gene_synonym=TREA /db_xref="GeneID:11181" /db_xref="HGNC:12266" /db_xref="MIM:275360" CDS 673..2244 /dnas_title="TREH protein" /gene="TREH" /gene_synonym=TRE /gene_synonym=TREA /codon_start=1 /product="TREH protein" /protein_id="AAI09207.1" /db_xref="GI:80475840" /db_xref="GeneID:11181" /db_xref="HGNC:12266" /db_xref="MIM:275360" /translation="MPGRTWELCLLLLLGLGLGSQEALPPPCESEIYCHGELLNQVQM AKLYQDDKQFVDMPLSIAPEQVLQTFTELSRDHNHSIPREQLQAFVHEHFQAKGQELQ PWTPADWKDSPQFLQKISDAKLRAWAGQLHQLWKKLGKKMKPEVLSHPERFSLIYSEH PFIVPGGRFVEFYYCYGHVPNGGRVYYLQRSQPPLLTLMMDCYLTHTNDTAFLQENIE TLALELDFWTKNRTVSVSLEGKNYLLNRYYVPYGGPRPESYSKDVELADTLPEGDREA LWAELKAGAESGWDFSSRWLIGGPNPNSLSGIRTSKLVPVDLNAFLCQAEELMSNFYS RLGNDSQATKYRILRSQRLAALNTVLWDEQTGAWFDYDLEKKKKNREFYPSNLTPLWA GCFSDPGVADKALKYLEDNRILTYQYGIPTSLQKTGQQWDFPNAWAPLQDLVIRGLAK APLRRAQEVAFQLAQNWIRTNFDVYSQKSAMYEKYDVSNGGQPGGGGEYEVQEGFGWT NGVVLMLLDRYGDRLTSGAKLAFLEPHCLAATLLPSLLLSLLPW" misc_feature 673..2244 /note="TREH coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgccagg gaggacctgg gagctgtgcc tgctactgct gctggggctg 721 ggactggggt cccaggaggc cctaccccca ccctgtgaga gtgagattta ctgccacggg 781 gagctcctaa accaagttca aatggccaag ctctaccagg atgacaagca gtttgtggac 841 atgccactgt ctatagctcc agaacaagtc ctgcagacct tcactgagct gtccagggac 901 cacaatcaca gcatccccag ggagcagctg caggcgtttg tccacgaaca cttccaggcc 961 aaggggcagg agctgcagcc ctggacccct gcagactgga aagacagccc ccagttcctg 1021 cagaagattt cagatgccaa actgcgtgcc tgggcagggc agctgcatca gctctggaag 1081 aagctgggga agaagatgaa gccagaggtt ctcagccacc ctgagcggtt ctctctcatc 1141 tactcagaac atcccttcat tgtgcctggc ggtcgctttg ttgagttcta ctactgctat 1201 gggcatgtcc ccaatggtgg gcgcgtgtac tacctgcagc ggagccagcc cccactcttg 1261 accctcatga tggattgcta cttgactcac accaatgaca ccgcctttct acaggaaaac 1321 attgaaacac tagccttgga attggacttt tggaccaaga acaggactgt ctctgtgagc 1381 ttggagggaa agaactacct cctgaatcgc tattatgtcc cttatggggg acccaggcct 1441 gagtcctaca gcaaagatgt ggagttggct gacaccttgc cagaaggaga ccgggaggct 1501 ctgtgggctg agctcaaggc tggggctgag tctggctggg acttctcttc acgctggctc 1561 attggaggcc caaaccccaa ctcgcttagc ggcatccgaa caagcaaact ggtgcctgtt 1621 gacctgaatg ccttcctatg ccaagcagag gagctgatga gcaacttcta ttccaggctg 1681 gggaacgact cccaggccac gaagtacaga atcctgcggt cgcagcgctt ggccgccctg 1741 aacacagtcc tgtgggatga gcagaccgga gcctggttcg attacgacct tgagaagaag 1801 aagaaaaacc gggagtttta cccatccaac ctcactccac tctgggccgg gtgtttctct 1861 gaccctggcg tggcggacaa ggctctgaaa tacctggagg acaaccggat cctgacttac 1921 cagtatggga tcccgacctc tctccagaag acaggccagc agtgggattt ccccaatgcc 1981 tgggcccccc tgcaggacct ggtcatcaga ggcctggcca aggcaccttt acgtcgggcc 2041 caggaagtgg ctttccagct ggctcagaat tggatccgaa ccaattttga tgtctactcg 2101 cagaagtcag ccatgtatga gaagtatgac gtcagcaacg gtggacagcc cggtggggga 2161 ggagaatatg aagttcagga gggatttggc tggacgaatg gcgtggtcct gatgctgctg 2221 gaccgctatg gtgaccggct gaccgaaaac ttgtactttc aaggctCCAG CTTTCTTGTA 2281 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT 2341 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA 2401 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT 2461 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA 2521 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA 2581 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC 2641 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA 2701 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT 2761 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC 2821 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 2881 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA 2941 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA 3001 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT 3061 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA 3121 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 3181 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA 3241 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT 3301 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG 3361 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA 3421 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 3481 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 3541 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 3601 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG 3661 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 3721 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 3781 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 3841 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 3901 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 3961 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 4021 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 4081 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT //