LOCUS TREH-pGEn1-DEST 6166 bp DNA circular 24-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4794..5335 /note="WPRE" misc_recomb 4756..4778 /label=attB2 /note="attB2 site" misc_feature complement(5342..5556) /note="BGH pA" misc_feature complement(5644..5773) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4755 /dnas_title="TREH-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:129621 IMAGE:40006501" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_283" /note="Vector: pCR-Blunt II-TOPO with reversed insert; Clone identification sequence tag: ATACAATA sequenced from the forward primer" gene 3166..4755 /dnas_title="TREH" /gene="TREH" /gene_synonym="TRE" /gene_synonym="TREA" /db_xref="GeneID:11181" /db_xref="HGNC:12266" /db_xref="MIM:275360" CDS 3166..4755 /dnas_title="TREH protein" /gene="TREH" /gene_synonym="TRE" /gene_synonym="TREA" /codon_start=1 /product="TREH protein" /protein_id="AAI09207.1" /db_xref="GI:80475840" /db_xref="GeneID:11181" /db_xref="HGNC:12266" /db_xref="MIM:275360" /translation="MPGRTWELCLLLLLGLGLGSQEALPPPCESEIYCHGELLNQVQM AKLYQDDKQFVDMPLSIAPEQVLQTFTELSRDHNHSIPREQLQAFVHEHFQAKGQELQ PWTPADWKDSPQFLQKISDAKLRAWAGQLHQLWKKLGKKMKPEVLSHPERFSLIYSEH PFIVPGGRFVEFYYCYGHVPNGGRVYYLQRSQPPLLTLMMDCYLTHTNDTAFLQENIE TLALELDFWTKNRTVSVSLEGKNYLLNRYYVPYGGPRPESYSKDVELADTLPEGDREA LWAELKAGAESGWDFSSRWLIGGPNPNSLSGIRTSKLVPVDLNAFLCQAEELMSNFYS RLGNDSQATKYRILRSQRLAALNTVLWDEQTGAWFDYDLEKKKKNREFYPSNLTPLWA GCFSDPGVADKALKYLEDNRILTYQYGIPTSLQKTGQQWDFPNAWAPLQDLVIRGLAK APLRRAQEVAFQLAQNWIRTNFDVYSQKSAMYEKYDVSNGGQPGGGGEYEVQEGFGWT NGVVLMLLDRYGDRLTSGAKLAFLEPHCLAATLLPSLLLSLLPW" misc_feature 3166..4755 /note="TREH coding region" misc_difference 4675..4675 /dnas_title="TREH" /gene="TREH" /gene_synonym="TRE" /gene_synonym="TREA" /note="'G' in cDNA is 'C' in the human genome; amino acid difference: 'A' in cDNA, 'P' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_difference 4684..4684 /dnas_title="TREH" /gene="TREH" /gene_synonym="TRE" /gene_synonym="TREA" /note="'G' in cDNA is 'C' in the human genome; amino acid difference: 'A' in cDNA, 'P' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_difference 4709..4709 /dnas_title="TREH" /gene="TREH" /gene_synonym="TRE" /gene_synonym="TREA" /note="1 base in cDNA is not found in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_difference 4737..4737 /dnas_title="TREH" /gene="TREH" /gene_synonym="TRE" /gene_synonym="TREA" /note="'C' in cDNA is 'A' in the human genome; no amino acid change. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." vector join(4764..6166,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4763 /source="TREH-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcctacc cccaccctgt 3181 gagagtgaga tttactgcca cggggagctc ctaaaccaag ttcaaatggc caagctctac 3241 caggatgaca agcagtttgt ggacatgcca ctgtctatag ctccagaaca agtcctgcag 3301 accttcactg agctgtccag ggaccacaat cacagcatcc ccagggagca gctgcaggcg 3361 tttgtccacg aacacttcca ggccaagggg caggagctgc agccctggac ccctgcagac 3421 tggaaagaca gcccccagtt cctgcagaag atttcagatg ccaaactgcg tgcctgggca 3481 gggcagctgc atcagctctg gaagaagctg gggaagaaga tgaagccaga ggttctcagc 3541 caccctgagc ggttctctct catctactca gaacatccct tcattgtgcc tggcggtcgc 3601 tttgttgagt tctactactg ctatgggcat gtccccaatg gtgggcgcgt gtactacctg 3661 cagcggagcc agcccccact cttgaccctc atgatggatt gctacttgac tcacaccaat 3721 gacaccgcct ttctacagga aaacattgaa acactagcct tggaattgga cttttggacc 3781 aagaacagga ctgtctctgt gagcttggag ggaaagaact acctcctgaa tcgctattat 3841 gtcccttatg ggggacccag gcctgagtcc tacagcaaag atgtggagtt ggctgacacc 3901 ttgccagaag gagaccggga ggctctgtgg gctgagctca aggctggggc tgagtctggc 3961 tgggacttct cttcacgctg gctcattgga ggcccaaacc ccaactcgct tagcggcatc 4021 cgaacaagca aactggtgcc tgttgacctg aatgccttcc tatgccaagc agaggagctg 4081 atgagcaact tctattccag gctggggaac gactcccagg ccacgaagta cagaatcctg 4141 cggtcgcagc gcttggccgc cctgaacaca gtcctgtggg atgagcagac cggagcctgg 4201 ttcgattacg accttgagaa gaagaagaaa aaccgggagt tttacccatc caacctcact 4261 ccactctggg ccgggtgttt ctctgaccct ggcgtggcgg acaaggctct gaaatacctg 4321 gaggacaacc ggatcctgac ttaccagtat gggatcccga cctctctcca gaagacaggc 4381 cagcagtggg atttccccaa tgcctgggcc cccctgcagg acctggtcat cagaggcctg 4441 gccaaggcac ctttacgtcg ggcccaggaa gtggctttcc agctggctca gaattggatc 4501 cgaaccaatt ttgatgtcta ctcgcagaag tcagccatgt atgagaagta tgacgtcagc 4561 aacggtggac agcccggtgg gggaggagaa tatgaagttc aggagggatt tggctggacg 4621 aatggcgtgg tcctgatgct gctggaccgc tatggtgacc ggctgacctc aggggccaag 4681 ctggctttcc tggagcccca ctgcctggcg gccacccttc tgcccagcct cctgctcagc 4741 ctcctgccat ggtgaCCAGC TTTCTTGTAC AAAGTGGTTG ATAGCTTGGA TCCAATCAAC 4801 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 4861 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 4921 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 4981 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 5041 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 5101 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 5161 CTGACAATTC CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG 5221 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 5281 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGAGATC 5341 TGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC 5401 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC 5461 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG 5521 GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGACTCG AGTTAAGGGC GAATTGCCGA 5581 TTAGGATCTT CCTAGAGCAT GGCTACGTAG ATAAGTAGCA TGGCGGGTTA ATCATTAACT 5641 ACAAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG 5701 AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG 5761 AGCGAGCGCG CAGCCTTAAT TAAGAGCTCA TGGCGCGCCT AGGCCTTGAC GGCCTTCCGC 5821 CAATTCGCCC TATAGTGAGT CGTATTACGT CGCGCTCACT GGCCGTCGTT TTACAACGTC 5881 GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG 5941 CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGAAACGCC CTTCCCAACA GTTGCGCAGC 6001 CTGAATGGCG AATGGGAGCG CCCTGTAGCG GCCACTCAAC CCTATCTCGG TCTATTCTTT 6061 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 6121 AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAG //