LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3728..3824) /label=attL2 /note="attL2 (c)" misc_signal complement(3839..3858) /note="T7 Promoter/priming site (c)" primer_bind 3866..3882 /note="M13 Reverse priming site" gene 3995..4804 /note="Kanamycin resistance gene" rep_origin 4925..5598 /note="pUC origin" PCR_frame 669..3727 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..3747) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..3744) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(3736..5601,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 3706..3726 /note="TEV site" source 673..3705 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2113 IMAGE:3504417" /tissue_type="Placenta, choriocarcinoma" /clone_lib="NIH_MGC_21" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..3705 /gene="MAN2B1" /gene_synonym=LAMAN /db_xref="GeneID:4125" /db_xref="HGNC:6826" /db_xref="MIM:609458" CDS 673..3705 /dnas_title="mannosidase, alpha, class 2B, member 1" /gene="MAN2B1" /gene_synonym=LAMAN /codon_start=1 /product="mannosidase, alpha, class 2B, member 1" /protein_id="AAH00736.1" /db_xref="GI:12653887" /db_xref="GeneID:4125" /db_xref="HGNC:6826" /db_xref="MIM:609458" /translation="MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAA AGARAGGYETCPTVQPNMLNVHLLPHTHDDVGWLKTVDQYFYGIKNDIQHAGVQYILD SVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVRDLVRQGRLEFANGGWVMNDE AATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGHSREQASLFAQMGFDGFFF GRLDYQDKWVRMQKLEMEQVWRASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQ PLVEDPRSPEYNAKELVDYFLNVATAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDK LIRLVNAQQAKGSSVHVLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYF SSRPALKRYERLSYNFLQVCNQLEALVGLAANVGPYGSGDSAPLNEAMAVLQHHDAVS GTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHFTFCQQLNISICPLSQTAA RFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAHPPELLF SASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLM EIMNMNQQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVK TPLVQEVHQNFSAWCSQVVRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETK GRFYTDSNGREILERRRDYRPTWKLNQTEPVAGNYYPVNTRIYITDGNMQLTVLTDRS QGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRGRHLVLLDTAQAAAAGH RLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGPEMVL LRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTT NTGPTPHQTPYQLDPANITLEPMEIRTFLASVQWKEVDG" misc_feature 673..3705 /note="MAN2B1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgggcgc ctacgcgcgg gcttcggggg tctgcgctcg cggctgcctg 721 gactcagcag gcccctggac catgtcccgc gccctgcggc caccgctccc gcctctctgc 781 tttttccttt tgttgctggc ggctgccggt gctcgggccg ggggatacga gacatgcccc 841 acagtgcagc cgaacatgct gaacgtgcac ctgctgcctc acacacatga tgacgtgggc 901 tggctcaaaa ccgtggacca gtacttttat ggaatcaaga atgacatcca gcacgccggt 961 gtgcagtaca tcctggactc ggtcatctct gccttgctgg cagatcccac ccgtcgcttc 1021 atttacgtgg agattgcctt cttctcccgt tggtggcacc agcagacaaa tgccacacag 1081 gaagtcgtgc gagaccttgt gcgccagggg cgcctggagt tcgccaatgg tggctgggtg 1141 atgaacgatg aggcagccac ccactacggt gccatcgtgg accagatgac acttgggctg 1201 cgctttctgg aggacacatt tggcaatgat gggcgacccc gtgtggcctg gcacattgac 1261 cccttcggcc actctcggga gcaggcctcg ctgtttgcgc agatgggctt cgacggcttc 1321 ttctttgggc gccttgatta tcaagataag tgggtacgga tgcagaagct ggagatggag 1381 caggtgtggc gggccagcac cagcctgaag cccccgaccg cggacctctt cactggtgtg 1441 cttcccaatg gttacaaccc gccaaggaat ctgtgctggg atgtgctgtg tgtcgatcag 1501 ccgctggtgg aggaccctcg cagccccgag tacaacgcca aggagctggt cgattacttc 1561 ctaaatgtgg ccactgccca gggccggtat taccgcacca accacactgt gatgaccatg 1621 ggctcggact tccaatatga gaatgccaac atgtggttca agaaccttga caagctcatc 1681 cggctggtaa atgcgcagca ggcaaaagga agcagtgtcc atgttctcta ctccaccccc 1741 gcttgttacc tctgggagct gaacaaggcc aacctcacct ggtcagtgaa acatgacgac 1801 ttcttccctt acgcggatgg cccccaccag ttctggaccg gttacttttc cagtcggccg 1861 gccctcaaac gctacgagcg cctcagctac aacttcctgc aggtgtgcaa ccagctggag 1921 gcgctggtgg gcctggcggc caacgtggga ccctatggct ccggagacag tgcacccctc 1981 aatgaggcga tggctgtgct ccagcatcac gacgccgtca gcggcacctc ccgccagcac 2041 gtggccaacg actacgcgcg ccagcttgcg gcaggctggg ggccttgcga ggttcttctg 2101 agcaacgcgc tggcgcggct cagaggcttc aaagatcact tcaccttttg ccaacagcta 2161 aacatcagca tctgcccgct cagccagacg gcggcgcgct tccaggtcat cgtttataat 2221 cccctggggc ggaaggtgaa ttggatggta cggctgccgg tcagcgaagg cgttttcgtt 2281 gtgaaggacc ccaatggcag gacagtgccc agcgatgtgg taatatttcc cagctcagac 2341 agccaggcgc accctccgga gctgctgttc tcagcctcac tgcccgccct gggcttcagc 2401 acctattcag tagcccaggt gcctcgctgg aagccccagg cccgcgcacc acagcccatc 2461 cccagaagat cctggtcccc tgctttaacc atcgaaaatg agcacatccg ggcaacgttt 2521 gatcctgaca cagggctgtt gatggagatt atgaacatga atcagcaact cctgctgcct 2581 gttcgccaga ccttcttctg gtacaacgcc agtataggtg acaacgaaag tgaccaggcc 2641 tcaggtgcct acatcttcag acccaaccaa cagaaaccgc tgcctgtgag ccgctgggct 2701 cagatccacc tggtgaagac acccttggtg caggaggtgc accagaactt ctcagcttgg 2761 tgttcccagg tggttcgcct gtacccagga cagcggcacc tggagctaga gtggtcggtg 2821 gggccgatac ctgtgggcga cacctggggg aaggaggtca tcagccgttt tgacacaccg 2881 ctggagacaa agggacgctt ctacacagac agcaatggcc gggagatcct ggagaggagg 2941 cgggattatc gacccacctg gaaactgaac cagacggagc ccgtggcagg aaactactat 3001 ccagtcaaca cccggattta catcacggat ggaaacatgc agctgactgt gctgactgac 3061 cgctcccagg ggggcagcag cctgagagat ggctcgctgg agctcatggt gcaccgaagg 3121 ctgctgaagg acgatggacg cggagtatcg gagccactaa tggagaacgg gtcgggggcg 3181 tgggtgcgag ggcgccacct ggtgctgctg gacacagccc aggctgcagc cgccggacac 3241 cggctcctgg cggagcagga ggtcctggcc cctcaggtgg tgctggcccc gggtggcggc 3301 gccgcctaca atctcggggc tcctccgcgc acgcagttct cagggctgcg cagggacctg 3361 ccgccctcgg tgcacctgct cacgctggcc agctggggcc ccgaaatggt gctgctgcgc 3421 ttggagcacc agtttgccgt aggagaggat tccggacgta acctgagcgc ccccgttacc 3481 ttgaacttga gggacctgtt ctccaccttc accatcaccc gcctgcagga gaccacgctg 3541 gtggccaacc agctccgcga ggcagcctcc aggctcaagt ggacaacaaa cacaggcccc 3601 acaccccacc aaactccgta ccagctggac ccggccaaca tcacgctgga acccatggaa 3661 atccgcactt tcctggcctc agttcaatgg aaggaggtgg atggtgaaaa cttgtacttt 3721 caaggctCCA GCTTTCTTGT ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG 3781 TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGATAT 3841 CCCCTATAGT GAGTCGTATT ACATGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC 3901 TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAATAATA TCATCATGAA CAATAAAACT 3961 GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC 4021 GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA 4081 TAATGTCGGG CAATCAGGTG CGACAATCTA TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA 4141 GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG 4201 ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGTACTCC 4261 TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGAAAA ACAGCATTCC AGGTATTAGA 4321 AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT 4381 GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA 4441 GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA 4501 TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA CTTTTGCCAT TCTCACCGGA 4561 TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT 4621 AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT 4681 CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA 4741 TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT 4801 CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC ATTACGCTGA CTTGACGGGA 4861 CGGCGCAAGC TCATGACCAA AATCCCTTAA CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC 4921 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 4981 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 5041 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT 5101 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 5161 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 5221 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 5281 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 5341 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 5401 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 5461 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 5521 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 5581 CTGGCCTTTT GCTCACATGT T //