LOCUS MAN2B1-pGEc2-DES 8254 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(7732..7861) /note="3'-ITR" misc_feature complement(7430..7644) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 6882..7423 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 6061..6083 /note="attB2" misc_feature 6861..6866 /note="BstB1 site" misc_feature 6084..6779 /note="Super GFP" misc_feature 6780..6830 /note="AviTag" misc_feature 6831..6854 /note="His Tag" PCR_frame 3002..6060 /note="Reading Frame" /set="Set 1" source 3006..6038 /dnas_title="MAN2B1-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2113 IMAGE:3504417" /tissue_type="Placenta, choriocarcinoma" /clone_lib="NIH_MGC_21" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..6038 /dnas_title="MAN2B1" /gene="MAN2B1" /gene_synonym="LAMAN" /db_xref="GeneID:4125" /db_xref="HGNC:6826" /db_xref="MIM:609458" CDS 3006..6038 /dnas_title="mannosidase, alpha, class 2B, member 1" /gene="MAN2B1" /gene_synonym="LAMAN" /codon_start=1 /product="mannosidase, alpha, class 2B, member 1" /protein_id="AAH00736.1" /db_xref="GI:12653887" /db_xref="GeneID:4125" /db_xref="HGNC:6826" /db_xref="MIM:609458" /translation="MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAA AGARAGGYETCPTVQPNMLNVHLLPHTHDDVGWLKTVDQYFYGIKNDIQHAGVQYILD SVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVRDLVRQGRLEFANGGWVMNDE AATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGHSREQASLFAQMGFDGFFF GRLDYQDKWVRMQKLEMEQVWRASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQ PLVEDPRSPEYNAKELVDYFLNVATAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDK LIRLVNAQQAKGSSVHVLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYF SSRPALKRYERLSYNFLQVCNQLEALVGLAANVGPYGSGDSAPLNEAMAVLQHHDAVS GTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHFTFCQQLNISICPLSQTAA RFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAHPPELLF SASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLM EIMNMNQQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVK TPLVQEVHQNFSAWCSQVVRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETK GRFYTDSNGREILERRRDYRPTWKLNQTEPVAGNYYPVNTRIYITDGNMQLTVLTDRS QGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRGRHLVLLDTAQAAAAGH RLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGPEMVL LRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTT NTGPTPHQTPYQLDPANITLEPMEIRTFLASVQWKEVDG" misc_feature 3006..6038 /note="MAN2B1 coding region" misc_feature 6039..6059 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..6068) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..6068) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(6069..8254,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..6068 /source="MAN2B1-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggg cgcctacgcg cgggcttcgg gggtctgcgc tcgcggctgc ctggactcag 3061 caggcccctg gaccatgtcc cgcgccctgc ggccaccgct cccgcctctc tgctttttcc 3121 ttttgttgct ggcggctgcc ggtgctcggg ccgggggata cgagacatgc cccacagtgc 3181 agccgaacat gctgaacgtg cacctgctgc ctcacacaca tgatgacgtg ggctggctca 3241 aaaccgtgga ccagtacttt tatggaatca agaatgacat ccagcacgcc ggtgtgcagt 3301 acatcctgga ctcggtcatc tctgccttgc tggcagatcc cacccgtcgc ttcatttacg 3361 tggagattgc cttcttctcc cgttggtggc accagcagac aaatgccaca caggaagtcg 3421 tgcgagacct tgtgcgccag gggcgcctgg agttcgccaa tggtggctgg gtgatgaacg 3481 atgaggcagc cacccactac ggtgccatcg tggaccagat gacacttggg ctgcgctttc 3541 tggaggacac atttggcaat gatgggcgac cccgtgtggc ctggcacatt gaccccttcg 3601 gccactctcg ggagcaggcc tcgctgtttg cgcagatggg cttcgacggc ttcttctttg 3661 ggcgccttga ttatcaagat aagtgggtac ggatgcagaa gctggagatg gagcaggtgt 3721 ggcgggccag caccagcctg aagcccccga ccgcggacct cttcactggt gtgcttccca 3781 atggttacaa cccgccaagg aatctgtgct gggatgtgct gtgtgtcgat cagccgctgg 3841 tggaggaccc tcgcagcccc gagtacaacg ccaaggagct ggtcgattac ttcctaaatg 3901 tggccactgc ccagggccgg tattaccgca ccaaccacac tgtgatgacc atgggctcgg 3961 acttccaata tgagaatgcc aacatgtggt tcaagaacct tgacaagctc atccggctgg 4021 taaatgcgca gcaggcaaaa ggaagcagtg tccatgttct ctactccacc cccgcttgtt 4081 acctctggga gctgaacaag gccaacctca cctggtcagt gaaacatgac gacttcttcc 4141 cttacgcgga tggcccccac cagttctgga ccggttactt ttccagtcgg ccggccctca 4201 aacgctacga gcgcctcagc tacaacttcc tgcaggtgtg caaccagctg gaggcgctgg 4261 tgggcctggc ggccaacgtg ggaccctatg gctccggaga cagtgcaccc ctcaatgagg 4321 cgatggctgt gctccagcat cacgacgccg tcagcggcac ctcccgccag cacgtggcca 4381 acgactacgc gcgccagctt gcggcaggct gggggccttg cgaggttctt ctgagcaacg 4441 cgctggcgcg gctcagaggc ttcaaagatc acttcacctt ttgccaacag ctaaacatca 4501 gcatctgccc gctcagccag acggcggcgc gcttccaggt catcgtttat aatcccctgg 4561 ggcggaaggt gaattggatg gtacggctgc cggtcagcga aggcgttttc gttgtgaagg 4621 accccaatgg caggacagtg cccagcgatg tggtaatatt tcccagctca gacagccagg 4681 cgcaccctcc ggagctgctg ttctcagcct cactgcccgc cctgggcttc agcacctatt 4741 cagtagccca ggtgcctcgc tggaagcccc aggcccgcgc accacagccc atccccagaa 4801 gatcctggtc ccctgcttta accatcgaaa atgagcacat ccgggcaacg tttgatcctg 4861 acacagggct gttgatggag attatgaaca tgaatcagca actcctgctg cctgttcgcc 4921 agaccttctt ctggtacaac gccagtatag gtgacaacga aagtgaccag gcctcaggtg 4981 cctacatctt cagacccaac caacagaaac cgctgcctgt gagccgctgg gctcagatcc 5041 acctggtgaa gacacccttg gtgcaggagg tgcaccagaa cttctcagct tggtgttccc 5101 aggtggttcg cctgtaccca ggacagcggc acctggagct agagtggtcg gtggggccga 5161 tacctgtggg cgacacctgg gggaaggagg tcatcagccg ttttgacaca ccgctggaga 5221 caaagggacg cttctacaca gacagcaatg gccgggagat cctggagagg aggcgggatt 5281 atcgacccac ctggaaactg aaccagacgg agcccgtggc aggaaactac tatccagtca 5341 acacccggat ttacatcacg gatggaaaca tgcagctgac tgtgctgact gaccgctccc 5401 aggggggcag cagcctgaga gatggctcgc tggagctcat ggtgcaccga aggctgctga 5461 aggacgatgg acgcggagta tcggagccac taatggagaa cgggtcgggg gcgtgggtgc 5521 gagggcgcca cctggtgctg ctggacacag cccaggctgc agccgccgga caccggctcc 5581 tggcggagca ggaggtcctg gcccctcagg tggtgctggc cccgggtggc ggcgccgcct 5641 acaatctcgg ggctcctccg cgcacgcagt tctcagggct gcgcagggac ctgccgccct 5701 cggtgcacct gctcacgctg gccagctggg gccccgaaat ggtgctgctg cgcttggagc 5761 accagtttgc cgtaggagag gattccggac gtaacctgag cgcccccgtt accttgaact 5821 tgagggacct gttctccacc ttcaccatca cccgcctgca ggagaccacg ctggtggcca 5881 accagctccg cgaggcagcc tccaggctca agtggacaac aaacacaggc cccacacccc 5941 accaaactcc gtaccagctg gacccggcca acatcacgct ggaacccatg gaaatccgca 6001 ctttcctggc ctcagttcaa tggaaggagg tggatggtga aaacttgtac tttcaaggct 6061 CCAGCTTTCT TGTACAAAGT GGTATGAGCA AGGGCGAGGA ACTGTTCACC GGCGTGGTGC 6121 CCATCCTGGT GGAACTGGAT GGCGACGTGA ACGGCCACAA GTTCTCTGTG CGGGGAGAGG 6181 GCGAAGGCGA CGCCACAAAT GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC 6241 TGCCCGTGCC TTGGCCTACC CTCGTGACCA CACTGACCTA CGGCGTGCAG TGCTTCAGCA 6301 GATACCCCGA CCATATGAAG CGGCACGACT TCTTCAAGAG CGCCATGCCC GAGGGCTACG 6361 TGCAGGAACG GACCATCAGC TTCAAGGACG ACGGCACCTA CAAGACCAGA GCCGAAGTGA 6421 AGTTCGAGGG CGACACCCTC GTGAACCGGA TCGAGCTGAA GGGCATCGAT TTCAAAGAGG 6481 ACGGCAACAT CCTGGGCCAC AAGCTGGAGT ACAACTTCAA CAGCCACAAC GTGTACATCA 6541 CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA GATCCGGCAC AACGTGGAAG 6601 ATGGCAGCGT GCAGCTGGCC GACCACTACC AGCAGAACAC CCCCATCGGA GATGGCCCCG 6661 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGAGCGT GCTGAGCAAG GACCCCAACG 6721 AGAAGCGGGA CCACATGGTG CTGCTGGAAT TCGTGACCGC CGCTGGCATC ACCCACGGCA 6781 TGAGCGGCCT GAACGACATC TTCGAGGCCC AGAAAATCGA GTGGCACGAG CACCACCATC 6841 ATCACCATCA CCACTGATGA TTCGAAAAAT AATAAGGATC CAATCAACCT CTGGATTACA 6901 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 6961 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 7021 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 7081 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 7141 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 7201 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 7261 TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA 7321 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 7381 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGAGATCTG CCTCGACTGT 7441 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA 7501 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG 7561 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA 7621 AGACAATAGC AGGCATGCTG GGGACTCGAG TTAAGGGCGA ATTGCCGATT AGGATCTTCC 7681 TAGAGCATGG CTACGTAGAT AAGTAGCATG GCGGGTTAAT CATTAACTAC AAGGAACCCC 7741 TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC 7801 CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA 7861 GCCTTAATTA AGAGCTCATG GCGCGCCTAG GCCTTGACGG CCTTCCGCCA ATTCGCCCTA 7921 TAGTGAGTCG TATTACGTCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA 7981 ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA 8041 ATAGCGAAGA GGCCCGCACC GAAACGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA 8101 TGGGAGCGCC CTGTAGCGGC CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG 8161 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC 8221 GAATTTTAAC AAAATATTAA CGCTTACAAT TTAG //