LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2591..2687) /label=attL2 /note="attL2 (c)" misc_signal complement(2702..2721) /note="T7 Promoter/priming site (c)" primer_bind 2729..2745 /note="M13 Reverse priming site" gene 2858..3667 /note="Kanamycin resistance gene" rep_origin 3788..4461 /note="pUC origin" vector join(2599..4464,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2590 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:29498 IMAGE:4863779" /tissue_type="Skin, melanotic melanoma, high MDR." /clone_lib="NIH_MGC_49" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2590 /gene="EDEM1" /gene_synonym=EDEM /gene_synonym=KIAA0212 /db_xref="GeneID:9695" /db_xref="HGNC:18967" /db_xref="MIM:607673" CDS 692..2590 /dnas_title="ER degradation enhancer, mannosidase alpha-like 1" /gene="EDEM1" /gene_synonym=EDEM /gene_synonym=KIAA0212 /codon_start=1 /product="ER degradation enhancer, mannosidase alpha-like 1" /protein_id="AAH19088.1" /db_xref="GI:17512212" /db_xref="GeneID:9695" /db_xref="HGNC:18967" /db_xref="MIM:607673" /translation="MQWRALVLGLVLLRLGLHGVLWLVFGLGPSMGFYQRFPLSFGFQ RLRSPDGPASPTSGPVGRPGGVSGPSWLQPPGTGAAQSPRKAPRRPGPGMCGPANWGY VLGGRGRGPDEYEKRYSGAFPPQLRAQMRDLARGMFVFGYDNYMAHAFPQDELNPIHC RGRGPDRGDPSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKD STVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFE NTKTGIPYPRVNLKTGVPPDTNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVK ALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMF NAAYQSIQNYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIG DVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNP FYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDN PVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVIN SSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI" misc_feature 692..2590 /note="EDEM1 coding region" misc_difference 1231..1231 /gene="EDEM1" /gene_synonym=EDEM /gene_synonym=KIAA0212 /note="'A' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgggctgggg cccagcatgg gcttctacca 721 gcgctttccg ctcagcttcg gcttccagcg tctgaggagc cccgacggcc ccgcgtcgcc 781 cacctcgggg cccgtgggcc ggcctggggg ggtatccggg ccgtcgtggc tgcagccgcc 841 ggggaccggg gcagcgcaga gcccgcgcaa ggctccgcgg cgtcctgggc cggggatgtg 901 cggcccagcc aactggggct acgtgctggg cggccggggc cgcggcccgg acgagtacga 961 gaagcgctac agcggcgcct tccctccgca gctgcgtgcc cagatgcgcg acctggcacg 1021 gggcatgttc gtctttggct acgacaacta catggctcac gccttccccc aggacgagct 1081 caaccccatc cactgccgcg gccgtgggcc cgaccgcggg gacccttcaa atctgaacat 1141 caatgatgta ctagggaact actcattgac tcttgttgat gcattggata cacttgcaat 1201 aatgggaaat tcatccgagt tccagaaagc agtcaagtta gtgatcaaca cagtttcatt 1261 tgacaaagat tccaccgtcc aagtctttga ggccacgata agggtcctgg gaagcctcct 1321 ttctgctcac agaataataa ctgactccaa gcagcccttt ggtgacatga caattaagga 1381 ctatgataat gagttgttat acatggccca tgacctggcg gtgcggctcc tccctgcttt 1441 tgaaaacacc aagacaggga ttccatatcc tcgggtgaat ctaaagacag gagttcctcc 1501 tgacaccaat aatgagacat gcacagcggg agccggttcc ctcctggtgg aatttgggat 1561 tctgagtcga ctcctggggg actccacatt tgagtgggtg gccagacgag cagtgaaagc 1621 cctttggaac ctccggagca atgatacagg attactaggc aatgtcgtga acattcagac 1681 gggccactgg gttggaaagc agagtggcct gggtgccggg ctggactcct tctatgaata 1741 cctcttgaaa tcttacattc tctttggaga aaaagaagac ctagaaatgt ttaatgctgc 1801 atatcagagt attcagaact acttaagaag agggcgggaa gcctgcaatg aaggagaagg 1861 agaccctcca ctctatgtca acgtgaacat gttcagtggg cagctgatga acacctggat 1921 tgactctctg caggcctttt tccctggact gcaggtgctg ataggagatg tggaagatgc 1981 catctgcctt catgccttct actatgccat atggaaacga tatggtgccc tccctgagag 2041 atataactgg cagctgcagg cccctgacgt tctcttctac ccactgagac cagagttagt 2101 ggaatccaca tatctcctct accaggcaac caagaatccc ttctacctcc atgtaggaat 2161 ggatattctg cagagtctgg aaaagtacac aaaagtcaag tgtgggtacg ccacgctgca 2221 tcacgtcatt gacaagtcca cagaagaccg gatggagagc ttctttctca gtgagacctg 2281 taaatatttg tatctgctgt ttgatgaaga caatccagta cacaagtctg gaaccagata 2341 catgttcaca acagagggac acattgtatc tgtggatgag catcttcggg aattgccatg 2401 gaaggaattc ttctctgaag agggagggca ggaccaaggg ggaaagtctg tgcacaggcc 2461 gaaacctcat gagttaaaag tcatcaactc cagctccaac tgcaatcgtg tacctgatga 2521 gaggaggtac tccctgccct taaagagcat ctacatgcga cagattgacc agatggttgg 2581 tttgatttga CCAGCTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT 2641 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA 2701 TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT 2761 GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAATA ATATCATCAT GAACAATAAA 2821 ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC 2881 GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG 2941 CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC 3001 AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT 3061 CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC 3121 TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT 3181 AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG 3241 GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC 3301 TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG 3361 TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC 3421 GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA 3481 ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC 3541 CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA 3601 ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT 3661 TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG 3721 GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC 3781 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 3841 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 3901 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 3961 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 4021 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 4081 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 4141 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 4201 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 4261 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 4321 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 4381 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 4441 TTGCTGGCCT TTTGCTCACA TGTT //