LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2429..2525) /label=attL2 /note="attL2 (c)" misc_signal complement(2540..2559) /note="T7 Promoter/priming site (c)" primer_bind 2567..2583 /note="M13 Reverse priming site" gene 2696..3505 /note="Kanamycin resistance gene" rep_origin 3626..4299 /note="pUC origin" PCR_frame 669..2429 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2449) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2446) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2437..4302,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2407..2427 /note="TEV site" source 673..2406 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:935 IMAGE:3051376" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_15" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2406 /gene="EDEM2" /gene_synonym=bA4204.1 /gene_synonym=FLJ10783 /db_xref="GeneID:55741" /db_xref="HGNC:15877" CDS 673..2406 /dnas_title="ER degradation enhancer, mannosidase alpha-like 2" /gene="EDEM2" /gene_synonym=bA4204.1 /gene_synonym=FLJ10783 /codon_start=1 /product="ER degradation enhancer, mannosidase alpha-like 2" /protein_id="AAH01371.1" /db_xref="GI:12655043" /db_xref="GeneID:55741" /db_xref="HGNC:15877" /translation="MPFRLLIPLGLLCALLPQHHGAPGPDGSAPDPAHYRERVKAMFY HAYDSYLENAFPFDELRPLTCDGHDTWGSFSLTLIDALDTLLILGNVSEFQRVVEVLQ DSVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEVEAGWPCSGPLLRMAEEAARK LLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIVEFATLSSLTGDPVFEDVA RVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSYFEYLVKGAILLQDKKL MAMFLEYNKAIRNYTRFDDWYLWVQMYKGTVSMPVFQSLEAYWPGLQSLIGDIDNAMR TFLNYYTVWKQFGGLPEFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDPTLLEL GRDAVESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLLFDPTNFIHNN GSTFDTVITPYGECILGAGGYIFNTEAHPIDPAALHCCQRLKEEQWEVEDLMREFYSL KRSRSKFQKNTVSSGPWEPPARPGTLFSPENHDQARERKPAKQKVPLLSCPSQPFTSK LALLGQVFLDSS" misc_feature 673..2406 /note="EDEM2 coding region" misc_difference 2038..2038 /gene="EDEM2" /gene_synonym=bA4204.1 /gene_synonym=FLJ10783 /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'T' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgccttt ccggctgctc atcccgctcg gcctcctgtg cgcgctgctg 721 cctcagcacc atggtgcgcc aggtcccgac ggctccgcgc cagatcccgc ccactacagg 781 gagcgagtca aggccatgtt ctaccacgcc tacgacagct acctggagaa tgcctttccc 841 ttcgatgagc tgcgacctct cacctgtgac gggcacgaca cctggggcag tttttctctg 901 actctaattg atgcactgga caccttgctg attttgggga atgtctcaga attccaaaga 961 gtggttgaag tgctccagga cagcgtggac tttgatattg atgtgaacgc ctctgtgttt 1021 gaaacaaaca ttcgagtggt aggaggactc ctgtctgctc atctgctctc caagaaggct 1081 ggggtggaag tagaggctgg atggccctgt tccgggcctc tcctgagaat ggctgaggag 1141 gcggcccgaa aactcctccc agcctttcag acccccactg gcatgccata tggaacagtg 1201 aacttacttc atggcgtgaa cccaggagag acccctgtca cctgtacggc agggattggg 1261 accttcattg ttgaatttgc caccctgagc agcctcactg gtgacccggt gttcgaagat 1321 gtggccagag tggctttgat gcgcctctgg gagagccggt cagatatcgg gctggtcggc 1381 aaccacattg atgtgctcac tggcaagtgg gtggcccagg acgcaggcat cggggctggc 1441 gtggactcct actttgagta cttggtgaaa ggagccatcc tgcttcagga taagaagctc 1501 atggccatgt tcctagagta taacaaagcc atccggaact acacccgctt cgatgactgg 1561 tacctgtggg ttcagatgta caaggggact gtgtccatgc cagtcttcca gtccttggag 1621 gcctactggc ctggtcttca gagcctcatt ggagacattg acaatgccat gaggaccttc 1681 ctcaactact acactgtatg gaagcagttt ggggggctcc cggaattcta caacattcct 1741 cagggataca cagtggagaa gcgagagggc tacccacttc ggccagaact tattgaaagc 1801 gcaatgtacc tctaccgtgc cacgggggat cccaccctcc tagaactcgg aagagatgct 1861 gtggaatcca ttgaaaaaat cagcaaggtg gagtgcggat ttgcaacaat caaagatctg 1921 cgagaccaca agctggacaa ccgcatggag tcgttcttcc tggccgagac tgtgaaatac 1981 ctctacctcc tgtttgaccc aaccaacttc atccacaaca atgggtccac cttcgacacg 2041 gtgatcaccc cctatgggga gtgcatcctg ggggctgggg ggtacatctt caacacagaa 2101 gctcacccca tcgaccctgc cgccctgcac tgctgccaga ggctgaagga agagcagtgg 2161 gaggtggagg acttgatgag ggaattctac tctctcaaac ggagcaggtc gaaatttcag 2221 aaaaacactg ttagttcggg gccatgggaa cctccagcaa ggccaggaac actcttctca 2281 ccagaaaacc atgaccaggc aagggagagg aagcctgcca aacagaaggt cccacttctc 2341 agctgcccca gtcagccctt cacctccaag ttggcattac tgggacaggt tttcctagac 2401 tcctcagaaa acttgtactt tcaaggctCC AGCTTTCTTG TACAAAGTTG GCATTATAAG 2461 AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT 2521 TATTTGCCAT CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC 2581 CTGGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAATAAT 2641 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG 2701 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA 2761 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA 2821 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA 2881 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT 2941 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA 3001 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT 3061 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA 3121 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG 3181 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA 3241 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT 3301 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA 3361 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA 3421 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA 3481 TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG 3541 CATTACGCTG ACTTGACGGG ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA 3601 CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 3661 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 3721 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 3781 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 3841 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 3901 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 3961 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 4021 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 4081 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 4141 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 4201 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 4261 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT //