LOCUS EDEM2-pGEc2-DEST 6955 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6433..6562) /note="3'-ITR" misc_feature complement(6131..6345) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5583..6124 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4762..4784 /note="attB2" misc_feature 5562..5567 /note="BstB1 site" misc_feature 4785..5480 /note="Super GFP" misc_feature 5481..5531 /note="AviTag" misc_feature 5532..5555 /note="His Tag" PCR_frame 3002..4762 /note="Reading Frame" /set="Set 1" source 3006..4739 /dnas_title="EDEM2-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:935 IMAGE:3051376" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_15" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4739 /dnas_title="EDEM2" /gene="EDEM2" /gene_synonym="bA4204.1" /gene_synonym="FLJ10783" /db_xref="GeneID:55741" /db_xref="HGNC:15877" CDS 3006..4739 /dnas_title="ER degradation enhancer, mannosidase alpha-like 2" /gene="EDEM2" /gene_synonym="bA4204.1" /gene_synonym="FLJ10783" /codon_start=1 /product="ER degradation enhancer, mannosidase alpha-like 2" /protein_id="AAH01371.1" /db_xref="GI:12655043" /db_xref="GeneID:55741" /db_xref="HGNC:15877" /translation="MPFRLLIPLGLLCALLPQHHGAPGPDGSAPDPAHYRERVKAMFY HAYDSYLENAFPFDELRPLTCDGHDTWGSFSLTLIDALDTLLILGNVSEFQRVVEVLQ DSVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEVEAGWPCSGPLLRMAEEAARK LLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIVEFATLSSLTGDPVFEDVA RVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSYFEYLVKGAILLQDKKL MAMFLEYNKAIRNYTRFDDWYLWVQMYKGTVSMPVFQSLEAYWPGLQSLIGDIDNAMR TFLNYYTVWKQFGGLPEFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDPTLLEL GRDAVESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLLFDPTNFIHNN GSTFDTVITPYGECILGAGGYIFNTEAHPIDPAALHCCQRLKEEQWEVEDLMREFYSL KRSRSKFQKNTVSSGPWEPPARPGTLFSPENHDQARERKPAKQKVPLLSCPSQPFTSK LALLGQVFLDSS" misc_feature 3006..4739 /note="EDEM2 coding region" misc_feature 4740..4760 /note="TEV site" misc_difference 4371..4371 /dnas_title="EDEM2" /gene="EDEM2" /gene_synonym="bA4204.1" /gene_synonym="FLJ10783" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'T' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4769) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4769) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4770..6955,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4769 /source="EDEM2-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgcc tttccggctg ctcatcccgc tcggcctcct gtgcgcgctg ctgcctcagc 3061 accatggtgc gccaggtccc gacggctccg cgccagatcc cgcccactac agggagcgag 3121 tcaaggccat gttctaccac gcctacgaca gctacctgga gaatgccttt cccttcgatg 3181 agctgcgacc tctcacctgt gacgggcacg acacctgggg cagtttttct ctgactctaa 3241 ttgatgcact ggacaccttg ctgattttgg ggaatgtctc agaattccaa agagtggttg 3301 aagtgctcca ggacagcgtg gactttgata ttgatgtgaa cgcctctgtg tttgaaacaa 3361 acattcgagt ggtaggagga ctcctgtctg ctcatctgct ctccaagaag gctggggtgg 3421 aagtagaggc tggatggccc tgttccgggc ctctcctgag aatggctgag gaggcggccc 3481 gaaaactcct cccagccttt cagaccccca ctggcatgcc atatggaaca gtgaacttac 3541 ttcatggcgt gaacccagga gagacccctg tcacctgtac ggcagggatt gggaccttca 3601 ttgttgaatt tgccaccctg agcagcctca ctggtgaccc ggtgttcgaa gatgtggcca 3661 gagtggcttt gatgcgcctc tgggagagcc ggtcagatat cgggctggtc ggcaaccaca 3721 ttgatgtgct cactggcaag tgggtggccc aggacgcagg catcggggct ggcgtggact 3781 cctactttga gtacttggtg aaaggagcca tcctgcttca ggataagaag ctcatggcca 3841 tgttcctaga gtataacaaa gccatccgga actacacccg cttcgatgac tggtacctgt 3901 gggttcagat gtacaagggg actgtgtcca tgccagtctt ccagtccttg gaggcctact 3961 ggcctggtct tcagagcctc attggagaca ttgacaatgc catgaggacc ttcctcaact 4021 actacactgt atggaagcag tttggggggc tcccggaatt ctacaacatt cctcagggat 4081 acacagtgga gaagcgagag ggctacccac ttcggccaga acttattgaa agcgcaatgt 4141 acctctaccg tgccacgggg gatcccaccc tcctagaact cggaagagat gctgtggaat 4201 ccattgaaaa aatcagcaag gtggagtgcg gatttgcaac aatcaaagat ctgcgagacc 4261 acaagctgga caaccgcatg gagtcgttct tcctggccga gactgtgaaa tacctctacc 4321 tcctgtttga cccaaccaac ttcatccaca acaatgggtc caccttcgac acggtgatca 4381 ccccctatgg ggagtgcatc ctgggggctg gggggtacat cttcaacaca gaagctcacc 4441 ccatcgaccc tgccgccctg cactgctgcc agaggctgaa ggaagagcag tgggaggtgg 4501 aggacttgat gagggaattc tactctctca aacggagcag gtcgaaattt cagaaaaaca 4561 ctgttagttc ggggccatgg gaacctccag caaggccagg aacactcttc tcaccagaaa 4621 accatgacca ggcaagggag aggaagcctg ccaaacagaa ggtcccactt ctcagctgcc 4681 ccagtcagcc cttcacctcc aagttggcat tactgggaca ggttttccta gactcctcag 4741 aaaacttgta ctttcaaggc tCCAGCTTTC TTGTACAAAG TGGTATGAGC AAGGGCGAGG 4801 AACTGTTCAC CGGCGTGGTG CCCATCCTGG TGGAACTGGA TGGCGACGTG AACGGCCACA 4861 AGTTCTCTGT GCGGGGAGAG GGCGAAGGCG ACGCCACAAA TGGCAAGCTG ACCCTGAAGT 4921 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CTTGGCCTAC CCTCGTGACC ACACTGACCT 4981 ACGGCGTGCA GTGCTTCAGC AGATACCCCG ACCATATGAA GCGGCACGAC TTCTTCAAGA 5041 GCGCCATGCC CGAGGGCTAC GTGCAGGAAC GGACCATCAG CTTCAAGGAC GACGGCACCT 5101 ACAAGACCAG AGCCGAAGTG AAGTTCGAGG GCGACACCCT CGTGAACCGG ATCGAGCTGA 5161 AGGGCATCGA TTTCAAAGAG GACGGCAACA TCCTGGGCCA CAAGCTGGAG TACAACTTCA 5221 ACAGCCACAA CGTGTACATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA 5281 AGATCCGGCA CAACGTGGAA GATGGCAGCG TGCAGCTGGC CGACCACTAC CAGCAGAACA 5341 CCCCCATCGG AGATGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGAGCG 5401 TGCTGAGCAA GGACCCCAAC GAGAAGCGGG ACCACATGGT GCTGCTGGAA TTCGTGACCG 5461 CCGCTGGCAT CACCCACGGC ATGAGCGGCC TGAACGACAT CTTCGAGGCC CAGAAAATCG 5521 AGTGGCACGA GCACCACCAT CATCACCATC ACCACTGATG ATTCGAAAAA TAATAAGGAT 5581 CCAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG 5641 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC 5701 GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT 5761 TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA 5821 CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC 5881 CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC 5941 TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC 6001 TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC 6061 TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC 6121 TTCGAGATCT GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC 6181 CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA 6241 AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA 6301 CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGACTCGA GTTAAGGGCG 6361 AATTGCCGAT TAGGATCTTC CTAGAGCATG GCTACGTAGA TAAGTAGCAT GGCGGGTTAA 6421 TCATTAACTA CAAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT 6481 CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT 6541 CAGTGAGCGA GCGAGCGCGC AGCCTTAATT AAGAGCTCAT GGCGCGCCTA GGCCTTGACG 6601 GCCTTCCGCC AATTCGCCCT ATAGTGAGTC GTATTACGTC GCGCTCACTG GCCGTCGTTT 6661 TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC 6721 CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGAAACGCCC TTCCCAACAG 6781 TTGCGCAGCC TGAATGGCGA ATGGGAGCGC CCTGTAGCGG CCACTCAACC CTATCTCGGT 6841 CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT 6901 GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAG //