LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2366..2462) /label=attL2 /note="attL2 (c)" misc_signal complement(2477..2496) /note="T7 Promoter/priming site (c)" primer_bind 2504..2520 /note="M13 Reverse priming site" gene 2633..3442 /note="Kanamycin resistance gene" rep_origin 3563..4236 /note="pUC origin" vector join(2374..4239,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2365 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:935 IMAGE:3051376" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_15" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2365 /gene="EDEM2" /gene_synonym=bA4204.1 /gene_synonym=FLJ10783 /db_xref="GeneID:55741" /db_xref="HGNC:15877" CDS 692..2365 /dnas_title="ER degradation enhancer, mannosidase alpha-like 2" /gene="EDEM2" /gene_synonym=bA4204.1 /gene_synonym=FLJ10783 /codon_start=1 /product="ER degradation enhancer, mannosidase alpha-like 2" /protein_id="AAH01371.1" /db_xref="GI:12655043" /db_xref="GeneID:55741" /db_xref="HGNC:15877" /translation="MPFRLLIPLGLLCALLPQHHGAPGPDGSAPDPAHYRERVKAMFY HAYDSYLENAFPFDELRPLTCDGHDTWGSFSLTLIDALDTLLILGNVSEFQRVVEVLQ DSVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEVEAGWPCSGPLLRMAEEAARK LLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIVEFATLSSLTGDPVFEDVA RVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSYFEYLVKGAILLQDKKL MAMFLEYNKAIRNYTRFDDWYLWVQMYKGTVSMPVFQSLEAYWPGLQSLIGDIDNAMR TFLNYYTVWKQFGGLPEFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDPTLLEL GRDAVESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLLFDPTNFIHNN GSTFDTVITPYGECILGAGGYIFNTEAHPIDPAALHCCQRLKEEQWEVEDLMREFYSL KRSRSKFQKNTVSSGPWEPPARPGTLFSPENHDQARERKPAKQKVPLLSCPSQPFTSK LALLGQVFLDSS" misc_feature 692..2365 /note="EDEM2 coding region" misc_difference 1994..1994 /gene="EDEM2" /gene_synonym=bA4204.1 /gene_synonym=FLJ10783 /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'T' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgcgccaggt cccgacggct ccgcgccaga 721 tcccgcccac tacagggagc gagtcaaggc catgttctac cacgcctacg acagctacct 781 ggagaatgcc tttcccttcg atgagctgcg acctctcacc tgtgacgggc acgacacctg 841 gggcagtttt tctctgactc taattgatgc actggacacc ttgctgattt tggggaatgt 901 ctcagaattc caaagagtgg ttgaagtgct ccaggacagc gtggactttg atattgatgt 961 gaacgcctct gtgtttgaaa caaacattcg agtggtagga ggactcctgt ctgctcatct 1021 gctctccaag aaggctgggg tggaagtaga ggctggatgg ccctgttccg ggcctctcct 1081 gagaatggct gaggaggcgg cccgaaaact cctcccagcc tttcagaccc ccactggcat 1141 gccatatgga acagtgaact tacttcatgg cgtgaaccca ggagagaccc ctgtcacctg 1201 tacggcaggg attgggacct tcattgttga atttgccacc ctgagcagcc tcactggtga 1261 cccggtgttc gaagatgtgg ccagagtggc tttgatgcgc ctctgggaga gccggtcaga 1321 tatcgggctg gtcggcaacc acattgatgt gctcactggc aagtgggtgg cccaggacgc 1381 aggcatcggg gctggcgtgg actcctactt tgagtacttg gtgaaaggag ccatcctgct 1441 tcaggataag aagctcatgg ccatgttcct agagtataac aaagccatcc ggaactacac 1501 ccgcttcgat gactggtacc tgtgggttca gatgtacaag gggactgtgt ccatgccagt 1561 cttccagtcc ttggaggcct actggcctgg tcttcagagc ctcattggag acattgacaa 1621 tgccatgagg accttcctca actactacac tgtatggaag cagtttgggg ggctcccgga 1681 attctacaac attcctcagg gatacacagt ggagaagcga gagggctacc cacttcggcc 1741 agaacttatt gaaagcgcaa tgtacctcta ccgtgccacg ggggatccca ccctcctaga 1801 actcggaaga gatgctgtgg aatccattga aaaaatcagc aaggtggagt gcggatttgc 1861 aacaatcaaa gatctgcgag accacaagct ggacaaccgc atggagtcgt tcttcctggc 1921 cgagactgtg aaatacctct acctcctgtt tgacccaacc aacttcatcc acaacaatgg 1981 gtccaccttc gacacggtga tcacccccta tggggagtgc atcctggggg ctggggggta 2041 catcttcaac acagaagctc accccatcga ccctgccgcc ctgcactgct gccagaggct 2101 gaaggaagag cagtgggagg tggaggactt gatgagggaa ttctactctc tcaaacggag 2161 caggtcgaaa tttcagaaaa acactgttag ttcggggcca tgggaacctc cagcaaggcc 2221 aggaacactc ttctcaccag aaaaccatga ccaggcaagg gagaggaagc ctgccaaaca 2281 gaaggtccca cttctcagct gccccagtca gcccttcacc tccaagttgg cattactggg 2341 acaggttttc ctagactcct cataaCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 2401 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 2461 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 2521 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 2581 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 2641 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 2701 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 2761 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 2821 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 2881 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 2941 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 3001 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 3061 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 3121 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 3181 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 3241 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 3301 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 3361 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 3421 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 3481 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 3541 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 3601 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 3661 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 3721 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 3781 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 3841 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 3901 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 3961 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 4021 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 4081 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 4141 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 4201 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //