LOCUS EDEM2-pGEn2-DEST 6975 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 5603..6144 /note="WPRE" misc_recomb 5565..5587 /label=attB2 /note="attB2 site" misc_feature complement(6151..6365) /note="BGH pA" misc_feature complement(6453..6582) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" source 3891..5564 /dnas_title="EDEM2-pGEn2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:935 IMAGE:3051376" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_15" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3891..5564 /dnas_title="EDEM2" /gene="EDEM2" /gene_synonym="bA4204.1" /gene_synonym="FLJ10783" /db_xref="GeneID:55741" /db_xref="HGNC:15877" CDS 3891..5564 /dnas_title="ER degradation enhancer, mannosidase alpha-like 2" /gene="EDEM2" /gene_synonym="bA4204.1" /gene_synonym="FLJ10783" /codon_start=1 /product="ER degradation enhancer, mannosidase alpha-like 2" /protein_id="AAH01371.1" /db_xref="GI:12655043" /db_xref="GeneID:55741" /db_xref="HGNC:15877" /translation="MPFRLLIPLGLLCALLPQHHGAPGPDGSAPDPAHYRERVKAMFY HAYDSYLENAFPFDELRPLTCDGHDTWGSFSLTLIDALDTLLILGNVSEFQRVVEVLQ DSVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEVEAGWPCSGPLLRMAEEAARK LLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIVEFATLSSLTGDPVFEDVA RVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSYFEYLVKGAILLQDKKL MAMFLEYNKAIRNYTRFDDWYLWVQMYKGTVSMPVFQSLEAYWPGLQSLIGDIDNAMR TFLNYYTVWKQFGGLPEFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDPTLLEL GRDAVESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLLFDPTNFIHNN GSTFDTVITPYGECILGAGGYIFNTEAHPIDPAALHCCQRLKEEQWEVEDLMREFYSL KRSRSKFQKNTVSSGPWEPPARPGTLFSPENHDQARERKPAKQKVPLLSCPSQPFTSK LALLGQVFLDSS" misc_feature 3891..5564 /note="EDEM2 coding region" misc_difference 5193..5193 /dnas_title="EDEM2" /gene="EDEM2" /gene_synonym="bA4204.1" /gene_synonym="FLJ10783" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'T' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." vector join(5573..6975,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..5572 /source="EDEM2-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc gcgccaggtc 3901 ccgacggctc cgcgccagat cccgcccact acagggagcg agtcaaggcc atgttctacc 3961 acgcctacga cagctacctg gagaatgcct ttcccttcga tgagctgcga cctctcacct 4021 gtgacgggca cgacacctgg ggcagttttt ctctgactct aattgatgca ctggacacct 4081 tgctgatttt ggggaatgtc tcagaattcc aaagagtggt tgaagtgctc caggacagcg 4141 tggactttga tattgatgtg aacgcctctg tgtttgaaac aaacattcga gtggtaggag 4201 gactcctgtc tgctcatctg ctctccaaga aggctggggt ggaagtagag gctggatggc 4261 cctgttccgg gcctctcctg agaatggctg aggaggcggc ccgaaaactc ctcccagcct 4321 ttcagacccc cactggcatg ccatatggaa cagtgaactt acttcatggc gtgaacccag 4381 gagagacccc tgtcacctgt acggcaggga ttgggacctt cattgttgaa tttgccaccc 4441 tgagcagcct cactggtgac ccggtgttcg aagatgtggc cagagtggct ttgatgcgcc 4501 tctgggagag ccggtcagat atcgggctgg tcggcaacca cattgatgtg ctcactggca 4561 agtgggtggc ccaggacgca ggcatcgggg ctggcgtgga ctcctacttt gagtacttgg 4621 tgaaaggagc catcctgctt caggataaga agctcatggc catgttccta gagtataaca 4681 aagccatccg gaactacacc cgcttcgatg actggtacct gtgggttcag atgtacaagg 4741 ggactgtgtc catgccagtc ttccagtcct tggaggccta ctggcctggt cttcagagcc 4801 tcattggaga cattgacaat gccatgagga ccttcctcaa ctactacact gtatggaagc 4861 agtttggggg gctcccggaa ttctacaaca ttcctcaggg atacacagtg gagaagcgag 4921 agggctaccc acttcggcca gaacttattg aaagcgcaat gtacctctac cgtgccacgg 4981 gggatcccac cctcctagaa ctcggaagag atgctgtgga atccattgaa aaaatcagca 5041 aggtggagtg cggatttgca acaatcaaag atctgcgaga ccacaagctg gacaaccgca 5101 tggagtcgtt cttcctggcc gagactgtga aatacctcta cctcctgttt gacccaacca 5161 acttcatcca caacaatggg tccaccttcg acacggtgat caccccctat ggggagtgca 5221 tcctgggggc tggggggtac atcttcaaca cagaagctca ccccatcgac cctgccgccc 5281 tgcactgctg ccagaggctg aaggaagagc agtgggaggt ggaggacttg atgagggaat 5341 tctactctct caaacggagc aggtcgaaat ttcagaaaaa cactgttagt tcggggccat 5401 gggaacctcc agcaaggcca ggaacactct tctcaccaga aaaccatgac caggcaaggg 5461 agaggaagcc tgccaaacag aaggtcccac ttctcagctg ccccagtcag cccttcacct 5521 ccaagttggc attactggga caggttttcc tagactcctc ataaCCAGCT TTCTTGTACA 5581 AAGTGGTTGA TAGCTTGGAT CCAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC 5641 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT 5701 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT 5761 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT 5821 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG 5881 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG 5941 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT 6001 GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT 6061 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC 6121 TCTGCGGCCT CTTCCGCGTC TTCGAGATCT GCCTCGACTG TGCCTTCTAG TTGCCAGCCA 6181 TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC 6241 CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG 6301 GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT 6361 GGGGACTCGA GTTAAGGGCG AATTGCCGAT TAGGATCTTC CTAGAGCATG GCTACGTAGA 6421 TAAGTAGCAT GGCGGGTTAA TCATTAACTA CAAGGAACCC CTAGTGATGG AGTTGGCCAC 6481 TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC 6541 GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCCTTAATT AAGAGCTCAT 6601 GGCGCGCCTA GGCCTTGACG GCCTTCCGCC AATTCGCCCT ATAGTGAGTC GTATTACGTC 6661 GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 6721 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 6781 CGAAACGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGAGCGC CCTGTAGCGG 6841 CCACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 6901 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA 6961 ACGCTTACAA TTTAG //