LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3407..3503) /label=attL2 /note="attL2 (c)" misc_signal complement(3518..3537) /note="T7 Promoter/priming site (c)" primer_bind 3545..3561 /note="M13 Reverse priming site" gene 3674..4483 /note="Kanamycin resistance gene" rep_origin 4604..5277 /note="pUC origin" vector join(3415..5280,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..3406 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:177686 IMAGE:9052669" /tissue_type="Brain, cerebellum, PCR rescued clones" /clone_lib="NIH_MGC_311" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: ATTGTCAG" CDS 701..3406 /dnas_title="Unknown (protein for MGC:177686)" /codon_start=1 /product="Unknown (protein for MGC:177686)" /protein_id="AAI44150.1" /db_xref="GI:219518151" /translation="MSREEKQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVR GQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVF ETNIRVLGGLLGGHSLAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYP RINLKFGIRKPEARTGTETDTCTACAGTLILEFAALSRFTGATIFEEYARKALDFLWE KRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHY DAIMRYISQPPLLLDVHIHKPMLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQ VIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNK YARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEA HLLPLWLSTTNQSISKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKN VVDKSCPRGIIRVEESFRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQL VQHAIQAASSIDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIVSHPFFGRVVLTAGP AQFGLDLSKHKETRGFVASSKPSNGCSELTNPEAVMGKIALIQRGQCMFAEKARNIQN AGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFSKEGSIILDAIREYE EVEVLLSDKAKDRAAILKGKMIPSYIINSNPEMENEEQPSSENDSQNQSGEQISSSSQ EVDLVDQESSEENSLNSHPESLSLADMDNAASISPSEQTSNPTENHETTNLNGECTDL DNQLQEQSETEEDSNPNVSWGKKVQPIDSILADWNEDIEAFEMMEKDEL" misc_feature 692..3406 /note="EDEM3 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgccgagccc atgagtaggg aggagaaaca 721 gaagcttggg aatcaagtac tggaaatgtt tgatcatgct tatggtaact atatggaaca 781 tgcttaccct gctgatgaac tcatgccttt aacctgtaga ggtcgagtta gaggccaaga 841 gccaagtcgc ggtgacgttg atgatgcctt gggaaaattt tctctgacac tgattgattc 901 tttggacact cttgtggttt taaataaaac taaagaattt gaagatgcag tgagaaaagt 961 tttaagagat gttaatttag ataacgatgt agtcgtatca gtctttgaaa caaacatcag 1021 agttcttggg ggtcttttgg gtgggcactc cctggcaatc atgctgaaag aaaaaggtga 1081 atatatgcag tggtacaatg atgaacttct ccaaatggca aagcagttag gttacaaact 1141 tttaccggct ttcaacacta ccagtggcct tccttatcca agaattaatt taaagtttgg 1201 catcagaaaa ccagaagctc ggacaggaac tgagacagat acctgtacag cttgtgcagg 1261 taccttgatc cttgaatttg ctgctttaag tcgattcaca ggagcaacaa tatttgagga 1321 atatgccaga aaagctcttg attttctctg ggaaaaaaga cagcgaagta gtaatttagt 1381 gggcgtgact ataaatattc atactggaga ttgggtacga aaagatagtg gagttggagc 1441 agggattgat tcatattatg aatatctgtt gaaagcctat gtcttgcttg gagatgacag 1501 ttttctggaa agatttaaca cacactatga tgccataatg aggtatatta gccagccacc 1561 tcttctactt gatgtgcata tccacaaacc aatgctgaat gctcggactt ggatggatgc 1621 tttgcttgcc ttcttcccag gcttgcaggt gttaaagggg gatattagac ctgctattga 1681 aactcatgaa atgttatatc aggtgattaa aaaacacaat tttctaccag aggcatttac 1741 cacagatttc agagtacact gggctcaaca tcctttaagg ccagaatttg cagaaagtac 1801 ctacttctta tataaagcta caggagatcc ttactacctt gaagtaggga agacacttat 1861 tgaaaattta aataaatatg ctagagtgcc ttgcggattt gctgccatga aggatgttcg 1921 tactggaagt catgaggaca gaatggattc tttcttcttg gctgaaatgt ttaaatatct 1981 ttacctgtta tttgctgata aagaagacat tatttttgac atagaagatt acatctttac 2041 aacagaagct catctgttac ctctttggct ctctactaca aatcaaagca tctctaaaaa 2101 gaacacaacc tcggaatata cagaactgga tgacagtaac ttcgattgga cttgtccaaa 2161 tactcagatc ctctttccta atgacccatt gtatgctcaa agtattcgtg agcccttgaa 2221 aaatgtggtg gataagagct gtcctagagg catcatcaga gtagaggaga gtttcaggag 2281 tggagctaaa ccccctctga gagccagaga tttcatggcc actaaccctg agcatttaga 2341 aatcctgaag aagatggggg tgagtttgat tcacctcaaa gatgggagag tccagttggt 2401 ccaacatgca atccaagctg ctagttcaat cgacgctgaa gatgggttga ggttcatgca 2461 ggagatgatt gaattgtcaa gtcagcaaca aaaagaacag cagctgcctc cacgagctgt 2521 acaaattgtt tcccacccat tttttggcag ggtagtattg actgctggac cagctcagtt 2581 tgggctggat ctgtctaaac ataaagagac aagaggattt gttgcaagca gtaaaccatc 2641 caatggttgt tcagagctta ctaacccaga ggcagtgatg ggaaaaatcg cactgataca 2701 aagaggacag tgcatgtttg cagaaaaggc acgcaacatc cagaatgctg gagccattgg 2761 tggcattgtt attgatgaca atgaggggag cagcagtgat actgcccctc tgttccagat 2821 ggcaggtgat ggaaaggata cagatgacat caagatcccc atgctgttct tattcagcaa 2881 agaaggaagt atcatactgg atgccatccg ggaatatgag gaggtagaag tgctcctctc 2941 tgataaagca aaagatcgag cagcaatatt aaaaggtaaa atgattccaa gctacattat 3001 taatagtaat cctgaaatgg aaaatgaaga acaaccatcc tctgaaaatg attctcagaa 3061 tcagagtggt gaacagattt catcaagttc tcaggaggtt gatttggttg atcaagagtc 3121 ttctgaggaa aattctctaa attctcaccc agaatcatta tctctagcag atatggacaa 3181 tgctgcaagc atttcccctt ctgaacagac ttctaatccc acagaaaacc atgagactac 3241 aaatcttaat ggtgaatgta cagatttaga taaccagctt caagaacaat cagaaactga 3301 ggaagattcc aatcctaatg ttagctgggg taaaaaggtc cagcctatag actccatatt 3361 agcagactgg aatgaagata tagaagcatt tgaaatgatg gagtgaCCAG CTTTCTTGTA 3421 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT 3481 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA 3541 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT 3601 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA 3661 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA 3721 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC 3781 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA 3841 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT 3901 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC 3961 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 4021 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA 4081 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA 4141 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT 4201 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA 4261 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 4321 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA 4381 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT 4441 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG 4501 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA 4561 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 4621 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 4681 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 4741 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG 4801 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 4861 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 4921 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 4981 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 5041 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 5101 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 5161 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 5221 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT //