LOCUS EDEM3-pGEn1-DEST 7291 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 5919..6460 /note="WPRE" misc_recomb 5881..5903 /label=attB2 /note="attB2 site" misc_feature complement(6467..6681) /note="BGH pA" misc_feature complement(6769..6898) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..5880 /dnas_title="EDEM3-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:177686 IMAGE:9052669" /tissue_type="Brain, cerebellum, PCR rescued clones" /clone_lib="NIH_MGC_311" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: ATTGTCAG" CDS 3175..5880 /dnas_title="Unknown (protein for MGC:177686)" /codon_start=1 /product="Unknown (protein for MGC:177686)" /protein_id="AAI44150.1" /db_xref="GI:219518151" /translation="MSREEKQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVR GQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVF ETNIRVLGGLLGGHSLAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYP RINLKFGIRKPEARTGTETDTCTACAGTLILEFAALSRFTGATIFEEYARKALDFLWE KRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHY DAIMRYISQPPLLLDVHIHKPMLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQ VIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNK YARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEA HLLPLWLSTTNQSISKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKN VVDKSCPRGIIRVEESFRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQL VQHAIQAASSIDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIVSHPFFGRVVLTAGP AQFGLDLSKHKETRGFVASSKPSNGCSELTNPEAVMGKIALIQRGQCMFAEKARNIQN AGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFSKEGSIILDAIREYE EVEVLLSDKAKDRAAILKGKMIPSYIINSNPEMENEEQPSSENDSQNQSGEQISSSSQ EVDLVDQESSEENSLNSHPESLSLADMDNAASISPSEQTSNPTENHETTNLNGECTDL DNQLQEQSETEEDSNPNVSWGKKVQPIDSILADWNEDIEAFEMMEKDEL" misc_feature 3166..5880 /note="EDEM3 coding region" vector join(5889..7291,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..5888 /source="EDEM3-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcgccga gcccatgagt 3181 agggaggaga aacagaagct tgggaatcaa gtactggaaa tgtttgatca tgcttatggt 3241 aactatatgg aacatgctta ccctgctgat gaactcatgc ctttaacctg tagaggtcga 3301 gttagaggcc aagagccaag tcgcggtgac gttgatgatg ccttgggaaa attttctctg 3361 acactgattg attctttgga cactcttgtg gttttaaata aaactaaaga atttgaagat 3421 gcagtgagaa aagttttaag agatgttaat ttagataacg atgtagtcgt atcagtcttt 3481 gaaacaaaca tcagagttct tgggggtctt ttgggtgggc actccctggc aatcatgctg 3541 aaagaaaaag gtgaatatat gcagtggtac aatgatgaac ttctccaaat ggcaaagcag 3601 ttaggttaca aacttttacc ggctttcaac actaccagtg gccttcctta tccaagaatt 3661 aatttaaagt ttggcatcag aaaaccagaa gctcggacag gaactgagac agatacctgt 3721 acagcttgtg caggtacctt gatccttgaa tttgctgctt taagtcgatt cacaggagca 3781 acaatatttg aggaatatgc cagaaaagct cttgattttc tctgggaaaa aagacagcga 3841 agtagtaatt tagtgggcgt gactataaat attcatactg gagattgggt acgaaaagat 3901 agtggagttg gagcagggat tgattcatat tatgaatatc tgttgaaagc ctatgtcttg 3961 cttggagatg acagttttct ggaaagattt aacacacact atgatgccat aatgaggtat 4021 attagccagc cacctcttct acttgatgtg catatccaca aaccaatgct gaatgctcgg 4081 acttggatgg atgctttgct tgccttcttc ccaggcttgc aggtgttaaa gggggatatt 4141 agacctgcta ttgaaactca tgaaatgtta tatcaggtga ttaaaaaaca caattttcta 4201 ccagaggcat ttaccacaga tttcagagta cactgggctc aacatccttt aaggccagaa 4261 tttgcagaaa gtacctactt cttatataaa gctacaggag atccttacta ccttgaagta 4321 gggaagacac ttattgaaaa tttaaataaa tatgctagag tgccttgcgg atttgctgcc 4381 atgaaggatg ttcgtactgg aagtcatgag gacagaatgg attctttctt cttggctgaa 4441 atgtttaaat atctttacct gttatttgct gataaagaag acattatttt tgacatagaa 4501 gattacatct ttacaacaga agctcatctg ttacctcttt ggctctctac tacaaatcaa 4561 agcatctcta aaaagaacac aacctcggaa tatacagaac tggatgacag taacttcgat 4621 tggacttgtc caaatactca gatcctcttt cctaatgacc cattgtatgc tcaaagtatt 4681 cgtgagccct tgaaaaatgt ggtggataag agctgtccta gaggcatcat cagagtagag 4741 gagagtttca ggagtggagc taaaccccct ctgagagcca gagatttcat ggccactaac 4801 cctgagcatt tagaaatcct gaagaagatg ggggtgagtt tgattcacct caaagatggg 4861 agagtccagt tggtccaaca tgcaatccaa gctgctagtt caatcgacgc tgaagatggg 4921 ttgaggttca tgcaggagat gattgaattg tcaagtcagc aacaaaaaga acagcagctg 4981 cctccacgag ctgtacaaat tgtttcccac ccattttttg gcagggtagt attgactgct 5041 ggaccagctc agtttgggct ggatctgtct aaacataaag agacaagagg atttgttgca 5101 agcagtaaac catccaatgg ttgttcagag cttactaacc cagaggcagt gatgggaaaa 5161 atcgcactga tacaaagagg acagtgcatg tttgcagaaa aggcacgcaa catccagaat 5221 gctggagcca ttggtggcat tgttattgat gacaatgagg ggagcagcag tgatactgcc 5281 cctctgttcc agatggcagg tgatggaaag gatacagatg acatcaagat ccccatgctg 5341 ttcttattca gcaaagaagg aagtatcata ctggatgcca tccgggaata tgaggaggta 5401 gaagtgctcc tctctgataa agcaaaagat cgagcagcaa tattaaaagg taaaatgatt 5461 ccaagctaca ttattaatag taatcctgaa atggaaaatg aagaacaacc atcctctgaa 5521 aatgattctc agaatcagag tggtgaacag atttcatcaa gttctcagga ggttgatttg 5581 gttgatcaag agtcttctga ggaaaattct ctaaattctc acccagaatc attatctcta 5641 gcagatatgg acaatgctgc aagcatttcc ccttctgaac agacttctaa tcccacagaa 5701 aaccatgaga ctacaaatct taatggtgaa tgtacagatt tagataacca gcttcaagaa 5761 caatcagaaa ctgaggaaga ttccaatcct aatgttagct ggggtaaaaa ggtccagcct 5821 atagactcca tattagcaga ctggaatgaa gatatagaag catttgaaat gatggagtga 5881 CCAGCTTTCT TGTACAAAGT GGTTGATAGC TTGGATCCAA TCAACCTCTG GATTACAAAA 5941 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG 6001 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT 6061 TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG 6121 GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT 6181 GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG 6241 CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG 6301 TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC 6361 TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC 6421 GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG AGATCTGCCT CGACTGTGCC 6481 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG 6541 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG 6601 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA 6661 CAATAGCAGG CATGCTGGGG ACTCGAGTTA AGGGCGAATT GCCGATTAGG ATCTTCCTAG 6721 AGCATGGCTA CGTAGATAAG TAGCATGGCG GGTTAATCAT TAACTACAAG GAACCCCTAG 6781 TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA 6841 AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC 6901 TTAATTAAGA GCTCATGGCG CGCCTAGGCC TTGACGGCCT TCCGCCAATT CGCCCTATAG 6961 TGAGTCGTAT TACGTCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC 7021 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA 7081 GCGAAGAGGC CCGCACCGAA ACGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 7141 GAGCGCCCTG TAGCGGCCAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT 7201 TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA 7261 TTTTAACAAA ATATTAACGC TTACAATTTA G //