LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2618..2714) /label=attL2 /note="attL2 (c)" misc_signal complement(2729..2748) /note="T7 Promoter/priming site (c)" primer_bind 2756..2772 /note="M13 Reverse priming site" gene 2885..3694 /note="Kanamycin resistance gene" rep_origin 3815..4488 /note="pUC origin" PCR_frame 669..2618 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2638) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2635) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2626..4491,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2596..2616 /note="TEV site" source 673..2595 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:71683 IMAGE:5263292" /tissue_type="Brain, hippocampus" /clone_lib="NIH_MGC_95" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 673..2595 /gene="MAN1A2" /gene_synonym=MAN1B /db_xref="GeneID:10905" /db_xref="HGNC:6822" /db_xref="MIM:604345" CDS 673..2595 /dnas_title="mannosidase, alpha, class 1A, member 2" /gene="MAN1A2" /gene_synonym=MAN1B /codon_start=1 /product="mannosidase, alpha, class 1A, member 2" /protein_id="AAH63300.1" /db_xref="GI:38969810" /db_xref="GeneID:10905" /db_xref="HGNC:6822" /db_xref="MIM:604345" /translation="MTTPALLPLSGRRIPPLNLGPPSFPHHRATLRLSEKFILLLILS AFITLCFGAFFFLPDSSKHKRFDLGLEDVLIPHVDAGKGAKNPGVFLIHGPDEHRHRE EEERLRNKIRADHEKALEEAKEKLRKSREEIRAEIQTEKNKVVQEMKIKENKPLPPVP IPNLVGIRGGDPEDNDIREKREKIKEMMKHAWDNYRTYGWGHNELRPIARKGHSPNIF GSSQMGATIVDALDTLYIMGLHDEFLDGQRWIEDNLDFSVNSEVSVFEVNIRFIGGLL AAYYLSGEEIFKIKAVQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILA EFGTLHMEFIHLSYLTGDLTYYKKVMHIRKLLQKMDRPNGLYPNYLNPRTGRWGQYHT SVGGLGDSFYEYLLKAWLMSDKTDHEARKMYDDAIEAIEKHLIKKSRGGLTFIGEWKN GHLEKKMGHLACFAGGMFALGADGSRADKAGHYLELGAEIARTCHESYDRTALKLGPE SFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEKYCR VNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPL PVLHLANTTLSGNPAVR" misc_feature 673..2595 /note="MAN1A2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgactac cccagccctg ctgcccctct ctggacgtag gataccacct 721 ctgaacctgg ggccgccttc cttcccacat cacagggcta ccttgagact ttctgagaag 781 tttattcttc tccttattct tagtgccttc atcactctgt gttttggggc attctttttc 841 cttccagact cttcaaaaca caaacgcttt gatttgggtt tagaagatgt gttaattcca 901 catgtagatg ccggtaaagg ggctaaaaac cccggagtct tcctgatcca tggacccgat 961 gaacatagac acagggaaga ggaagaacgt ctgagaaata aaattcgagc tgatcatgag 1021 aaggccttgg aagaagcaaa agaaaaatta agaaagtcaa gagaggaaat tcgagcagaa 1081 attcagacag agaaaaataa ggtagtccaa gaaatgaaga taaaagagaa caagccactg 1141 ccaccagtcc ctattcccaa ccttgtagga atacgtggtg gagacccaga agataatgac 1201 ataagagaga aaagggaaaa aattaaagag atgatgaaac atgcttggga taactatagg 1261 acatatgggt ggggacataa tgaactcaga cctattgcaa ggaaaggaca ctcccctaac 1321 atatttggaa gttcacaaat gggtgctacc atagtagatg ctttggatac cctttatatc 1381 atgggacttc atgatgaatt cctagatggg caaagatgga ttgaagacaa ccttgatttc 1441 agtgtgaatt cagaggtgtc tgtgtttgaa gtcaacattc gatttattgg aggcctactt 1501 gcagcatatt acctatcagg agaggagata ttcaagatta aagcagtgca attggctgag 1561 aaactccttc ctgcctttaa cacacctact gggattcctt gggcaatggt gaatttgaaa 1621 agtggagtag ggcgaaactg gggctgggca tctgcaggta gcagcattct ggctgaattt 1681 ggtacactac atatggagtt catccacctc agctacttga caggggacct gacttactac 1741 aaaaaggtta tgcacattcg gaaactactt cagaaaatgg atcgtccaaa tggtctttat 1801 ccaaattatt tgaaccccag aacagggcgc tggggtcagt atcatacatc tgtcggtggc 1861 ctgggagaca gtttttatga atacttactg aaagcatggt tgatgtcaga taaaacagac 1921 catgaggcaa gaaagatgta tgatgatgct attgaggcta tagaaaaaca tcttattaag 1981 aagtctcgtg gaggtcttac ctttattgga gaatggaaga atgggcactt ggaaaaaaag 2041 atggggcatt tggcctgctt tgctggggga atgtttgcac taggagcaga tggttccaga 2101 gcagataaag ctggtcatta tttagagcta ggggcagaaa ttgcacgtac ttgtcatgag 2161 tcatatgaca gaactgcatt aaagctaggt cctgaatcat tcaagtttga tggtgcagtg 2221 gaggctgtgg ctgtccggca ggctgaaaag tattatatcc tccgtccaga agtaattgaa 2281 acctattggt acctatggcg attcactcac gatccaagat acaggcagtg gggctgggaa 2341 gcagcactgg ccattgaaaa gtattgccga gttaatggtg ggttttctgg agtcaaagat 2401 gtatattcct ctactcctac acatgatgat gtacagcaga gcttttttct tgctgaaaca 2461 ttaaaatatt tgtatctgct gttctccggt gatgaccttt tacctttaga ccactgggtg 2521 tttaatacag aggctcaccc tctgcctgtg ttacatttag ccaacaccac actttcaggt 2581 aatcctgctg ttcgagaaaa cttgtacttt caaggctCCA GCTTTCTTGT ACAAAGTTGG 2641 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA 2701 TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT 2761 AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA 2821 TAAAATAATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG 2881 TGTTATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC 2941 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA 3001 TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT 3061 TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT 3121 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT 3181 CCCCGGAAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT 3241 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT 3301 TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT 3361 TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA 3421 AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT 3481 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG 3541 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC 3601 TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT 3661 GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA 3721 CTGGCAGAGC ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA 3781 CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC 3841 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC 3901 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT 3961 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT 4021 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC 4081 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA 4141 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC 4201 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG 4261 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA 4321 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT 4381 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA 4441 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T //