LOCUS MAN1A2-pGEc2-DES 7144 bp DNA circular 25-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6622..6751) /note="3'-ITR" misc_feature complement(6320..6534) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5772..6313 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4951..4973 /note="attB2" misc_feature 5751..5756 /note="BstB1 site" misc_feature 4974..5669 /note="Super GFP" misc_feature 5670..5720 /note="AviTag" misc_feature 5721..5744 /note="His Tag" PCR_frame 3002..4951 /note="Reading Frame" /set="Set 1" source 3006..4928 /dnas_title="MAN1A2-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:71683 IMAGE:5263292" /tissue_type="Brain, hippocampus" /clone_lib="NIH_MGC_95" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 3006..4928 /dnas_title="MAN1A2" /gene="MAN1A2" /gene_synonym="MAN1B" /db_xref="GeneID:10905" /db_xref="HGNC:6822" /db_xref="MIM:604345" CDS 3006..4928 /dnas_title="mannosidase, alpha, class 1A, member 2" /gene="MAN1A2" /gene_synonym="MAN1B" /codon_start=1 /product="mannosidase, alpha, class 1A, member 2" /protein_id="AAH63300.1" /db_xref="GI:38969810" /db_xref="GeneID:10905" /db_xref="HGNC:6822" /db_xref="MIM:604345" /translation="MTTPALLPLSGRRIPPLNLGPPSFPHHRATLRLSEKFILLLILS AFITLCFGAFFFLPDSSKHKRFDLGLEDVLIPHVDAGKGAKNPGVFLIHGPDEHRHRE EEERLRNKIRADHEKALEEAKEKLRKSREEIRAEIQTEKNKVVQEMKIKENKPLPPVP IPNLVGIRGGDPEDNDIREKREKIKEMMKHAWDNYRTYGWGHNELRPIARKGHSPNIF GSSQMGATIVDALDTLYIMGLHDEFLDGQRWIEDNLDFSVNSEVSVFEVNIRFIGGLL AAYYLSGEEIFKIKAVQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILA EFGTLHMEFIHLSYLTGDLTYYKKVMHIRKLLQKMDRPNGLYPNYLNPRTGRWGQYHT SVGGLGDSFYEYLLKAWLMSDKTDHEARKMYDDAIEAIEKHLIKKSRGGLTFIGEWKN GHLEKKMGHLACFAGGMFALGADGSRADKAGHYLELGAEIARTCHESYDRTALKLGPE SFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEKYCR VNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPL PVLHLANTTLSGNPAVR" misc_feature 3006..4928 /note="MAN1A2 coding region" misc_feature 4929..4949 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4958) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4958) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4959..7144,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4958 /source="MAN1A2-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgac taccccagcc ctgctgcccc tctctggacg taggatacca cctctgaacc 3061 tggggccgcc ttccttccca catcacaggg ctaccttgag actttctgag aagtttattc 3121 ttctccttat tcttagtgcc ttcatcactc tgtgttttgg ggcattcttt ttccttccag 3181 actcttcaaa acacaaacgc tttgatttgg gtttagaaga tgtgttaatt ccacatgtag 3241 atgccggtaa aggggctaaa aaccccggag tcttcctgat ccatggaccc gatgaacata 3301 gacacaggga agaggaagaa cgtctgagaa ataaaattcg agctgatcat gagaaggcct 3361 tggaagaagc aaaagaaaaa ttaagaaagt caagagagga aattcgagca gaaattcaga 3421 cagagaaaaa taaggtagtc caagaaatga agataaaaga gaacaagcca ctgccaccag 3481 tccctattcc caaccttgta ggaatacgtg gtggagaccc agaagataat gacataagag 3541 agaaaaggga aaaaattaaa gagatgatga aacatgcttg ggataactat aggacatatg 3601 ggtggggaca taatgaactc agacctattg caaggaaagg acactcccct aacatatttg 3661 gaagttcaca aatgggtgct accatagtag atgctttgga taccctttat atcatgggac 3721 ttcatgatga attcctagat gggcaaagat ggattgaaga caaccttgat ttcagtgtga 3781 attcagaggt gtctgtgttt gaagtcaaca ttcgatttat tggaggccta cttgcagcat 3841 attacctatc aggagaggag atattcaaga ttaaagcagt gcaattggct gagaaactcc 3901 ttcctgcctt taacacacct actgggattc cttgggcaat ggtgaatttg aaaagtggag 3961 tagggcgaaa ctggggctgg gcatctgcag gtagcagcat tctggctgaa tttggtacac 4021 tacatatgga gttcatccac ctcagctact tgacagggga cctgacttac tacaaaaagg 4081 ttatgcacat tcggaaacta cttcagaaaa tggatcgtcc aaatggtctt tatccaaatt 4141 atttgaaccc cagaacaggg cgctggggtc agtatcatac atctgtcggt ggcctgggag 4201 acagttttta tgaatactta ctgaaagcat ggttgatgtc agataaaaca gaccatgagg 4261 caagaaagat gtatgatgat gctattgagg ctatagaaaa acatcttatt aagaagtctc 4321 gtggaggtct tacctttatt ggagaatgga agaatgggca cttggaaaaa aagatggggc 4381 atttggcctg ctttgctggg ggaatgtttg cactaggagc agatggttcc agagcagata 4441 aagctggtca ttatttagag ctaggggcag aaattgcacg tacttgtcat gagtcatatg 4501 acagaactgc attaaagcta ggtcctgaat cattcaagtt tgatggtgca gtggaggctg 4561 tggctgtccg gcaggctgaa aagtattata tcctccgtcc agaagtaatt gaaacctatt 4621 ggtacctatg gcgattcact cacgatccaa gatacaggca gtggggctgg gaagcagcac 4681 tggccattga aaagtattgc cgagttaatg gtgggttttc tggagtcaaa gatgtatatt 4741 cctctactcc tacacatgat gatgtacagc agagcttttt tcttgctgaa acattaaaat 4801 atttgtatct gctgttctcc ggtgatgacc ttttaccttt agaccactgg gtgtttaata 4861 cagaggctca ccctctgcct gtgttacatt tagccaacac cacactttca ggtaatcctg 4921 ctgttcgaga aaacttgtac tttcaaggct CCAGCTTTCT TGTACAAAGT GGTATGAGCA 4981 AGGGCGAGGA ACTGTTCACC GGCGTGGTGC CCATCCTGGT GGAACTGGAT GGCGACGTGA 5041 ACGGCCACAA GTTCTCTGTG CGGGGAGAGG GCGAAGGCGA CGCCACAAAT GGCAAGCTGA 5101 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC TTGGCCTACC CTCGTGACCA 5161 CACTGACCTA CGGCGTGCAG TGCTTCAGCA GATACCCCGA CCATATGAAG CGGCACGACT 5221 TCTTCAAGAG CGCCATGCCC GAGGGCTACG TGCAGGAACG GACCATCAGC TTCAAGGACG 5281 ACGGCACCTA CAAGACCAGA GCCGAAGTGA AGTTCGAGGG CGACACCCTC GTGAACCGGA 5341 TCGAGCTGAA GGGCATCGAT TTCAAAGAGG ACGGCAACAT CCTGGGCCAC AAGCTGGAGT 5401 ACAACTTCAA CAGCCACAAC GTGTACATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG 5461 CCAACTTCAA GATCCGGCAC AACGTGGAAG ATGGCAGCGT GCAGCTGGCC GACCACTACC 5521 AGCAGAACAC CCCCATCGGA GATGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA 5581 CCCAGAGCGT GCTGAGCAAG GACCCCAACG AGAAGCGGGA CCACATGGTG CTGCTGGAAT 5641 TCGTGACCGC CGCTGGCATC ACCCACGGCA TGAGCGGCCT GAACGACATC TTCGAGGCCC 5701 AGAAAATCGA GTGGCACGAG CACCACCATC ATCACCATCA CCACTGATGA TTCGAAAAAT 5761 AATAAGGATC CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA 5821 ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA 5881 TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT 5941 ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG 6001 CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT 6061 TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG 6121 GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC 6181 CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC 6241 CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC 6301 TTCCGCGTCT TCGAGATCTG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG 6361 CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA 6421 AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT 6481 GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGACTCGAG 6541 TTAAGGGCGA ATTGCCGATT AGGATCTTCC TAGAGCATGG CTACGTAGAT AAGTAGCATG 6601 GCGGGTTAAT CATTAACTAC AAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC 6661 GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC 6721 GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCCTTAATTA AGAGCTCATG GCGCGCCTAG 6781 GCCTTGACGG CCTTCCGCCA ATTCGCCCTA TAGTGAGTCG TATTACGTCG CGCTCACTGG 6841 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG 6901 CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GAAACGCCCT 6961 TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGAGCGCC CTGTAGCGGC CACTCAACCC 7021 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 7081 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT 7141 TTAG //